LOCUS       sequence-269558-        8538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the CRY2 blue light-inducible domain fused to the VPR 
            transcriptional activation domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Lebar T, Lainscek D, Merljak E, Aupic J, Jerala R
  TITLE     A tunable orthogonal coiled-coil interaction toolbox for engineering
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2020 Jan 6. pii: 10.1038/s41589-019-0443-y. doi: 
            10.1038/s41589-019-0443-y.
  PUBMED    31907374
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Jan 6. pii: 10.1038/s41589-019-0443-y. doi: 
            10.1038/s41589-019-0443-y."
COMMENT     Sequence Label: pcDNA3-His:CRY2:nls:VPR
FEATURES             Location/Qualifiers
     source          1..8538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             953..970
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             971..2461
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="KMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQF
                     YPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYD
                     PVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIE
                     SVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNE
                     FIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEE
                     SADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLV
                     DAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYI
                     SGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKAS
                     GVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2465..2485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2510..2659
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3128..3484
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             3503..4072
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        complement(4091..4109)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4092..4109)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4129..4146)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4135..4359
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4405..4833
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4492..4511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4702..4723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4842..4862)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4847..5176
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5027..5162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5089..5108
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5243..6037
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5297..5316)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5907..5926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6211..6332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6248..6267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6302..6321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6381..6397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6381..6397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6394..6416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6405..6421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6429..6435,6436..6453,6454..6459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6474..6495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6612..6629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6783..7371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6863..6882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7542..8333,8334..8402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8165..8184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8403..8507)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca
      961 ccaccaccac aagatggaca aaaagactat agtttggttt agaagagacc taaggattga
     1021 ggataatcct gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg
     1081 gtgtcctgaa gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca
     1141 atcacttgct cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa
     1201 aacccacaac acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt
     1261 cgtctttaac cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa
     1321 gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg
     1381 ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg
     1441 cttagatatg tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac
     1501 tgcagcggct gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc
     1561 cgagaaaccg agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga
     1621 taagttacta aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa
     1681 agttgttggg aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt
     1741 cagacacgtt ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag
     1801 tgaaggagaa gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg
     1861 gtatatatgt ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt
     1921 tttcccttgg gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta
     1981 tccgttggtg gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat
     2041 aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat
     2101 gaagtatttc tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca
     2161 gtatatctct gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt
     2221 acaaggcgcc aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc
     2281 gagattgcca actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc
     2341 ttctggtgtg gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg
     2401 tgagctacta gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc
     2461 agatccaaaa aagaagagaa aggtagaggc cagcggttcg ggacgggctg acgcattgga
     2521 cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct
     2581 tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga
     2641 tttcgacctg gacatgctga ttaacactag tggcggctct ggcggcggct ccggaggctc
     2701 tagccagtac ctgcccgaca ccgacgaccg gcaccggatc gaggaaaagc ggaagcggac
     2761 ctacgagaca ttcaagagca tcatgaagaa gtcccccttc agcggcccca ccgaccctag
     2821 acctccacct agaagaatcg ccgtgcccag cagatccagc gccagcgtgc caaaacctgc
     2881 cccccagcct taccccttca ccagcagcct gagcaccatc aactacgacg agttccctac
     2941 catggtgttc cccagcggcc agatctctca ggcctctgct ctggctccag cccctcctca
     3001 ggtgctgcct caggctcctg ctcctgcacc agctccagcc atggtgtctg cactggctca
     3061 ggcaccagca cccgtgcctg tgctggctcc tggacctcca caggctgtgg ctccaccagc
     3121 ccctaaacct acacaggccg gcgagggcac actgtctgaa gctctgctgc agctgcagtt
     3181 cgacgacgag gatctgggag ccctgctggg aaacagcacc gatcctgccg tgttcaccga
     3241 cctggccagc gtggacaaca gcgagttcca gcagctgctg aaccagggca tccctgtggc
     3301 ccctcacacc accgagccca tgctgatgga ataccccgag gccatcaccc ggctcgtgac
     3361 aggcgctcag aggcctcctg atccagctcc tgcccctctg ggagcaccag gcctgcctaa
     3421 tggactgctg tctggcgacg aggacttcag ctctatcgcc gatatggatt tctcagcctt
     3481 gctgggctct ggcagcggca gccgggattc cagggaaggg atgtttttgc cgaagcctga
     3541 ggccggctcc gctattagtg acgtgtttga gggccgcgag gtgtgccagc caaaacgaat
     3601 ccggccattt catcctccag gaagtccatg ggccaaccgc ccactccccg ccagcctcgc
     3661 accaacacca accggtccag tacatgagcc agtcgggtca ctgaccccgg caccagtccc
     3721 tcagccactg gacccagcgc ccgcagtgac tcccgaggcc agtcacctgt tggaggaccc
     3781 cgatgaagag acgagccagg ctgtcaaagc ccttcgggag atggccgata ctgtgattcc
     3841 ccagaaggaa gaggctgcaa tctgtggcca aatggacctt tcccatccgc ccccaagggg
     3901 ccatctggat gagctgacaa ccacacttga gtccatgacc gaggatctga acctggactc
     3961 acccctgacc ccggaattga acgagattct ggataccttc ctgaacgacg agtgcctctt
     4021 gcatgccatg catatcagca caggactgtc catcttcgac acatctctgt tttgatctag
     4081 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
     4141 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4201 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4261 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4321 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4381 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4441 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4501 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4561 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4621 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4681 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4741 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4801 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4921 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4981 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5041 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5101 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5161 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     5221 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5281 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5341 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5401 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     5461 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5521 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5581 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5641 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5701 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5761 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5821 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5881 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5941 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6001 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6061 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6121 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6181 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6361 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8521 aaagtgccac ctgacgtc
//
