LOCUS       sequence-269563-        9714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Promoter 2 with bsa1 cut sites for inserting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Qu J, Prasad NK, Yu MA, Chen S, Lyden A, Herrera N, Silvis MR, 
            Crawford E, Looney MR, Peters JM, Rosenberg OS
  TITLE     Modulating pathogenesis with Mobile-CRISPRi.
  JOURNAL   J Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19.
  PUBMED    31481541
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19."
COMMENT     Sequence Label: PJQ48
FEATURES             Location/Qualifiers
     source          1..9714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(6..53)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(170..175,176..192,193..198)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             387..920
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    complement(968..1015)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(1066..1152)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(1255..1349)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1377..1393)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1378..1394)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1808..1827)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1927..1949
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1987..2005)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2073..2177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2178..2246,2247..3038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2396..2415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3339..3727
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            3996..4104
                     /note="incP origin of transfer"
     mobile_element  4263..4428
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(4583..4672)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(4682..6265,6266..6268,6269..8755,8756..8758,
                     8759..8785))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4682..8785)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             8792..8803
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(8809..8843)
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     promoter        join(9276..9281,9282..9298,9299..9304)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctcaagaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcctcctga
       61 acggccataa gaacgaaggc tgtctgttga actctcgagc cttgtcgcct tgcgtataat
      121 atttgcccat ggacgcacac cgtggaaacg gatgaaggca cgaacccagt tgacataagc
      181 ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      361 atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg
      421 gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc
      481 tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg
      541 gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc
      601 gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg
      661 cggcttacgt tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg
      721 cagtctccgg agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc
      781 atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc
      841 ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg
      901 acccaagtac cgccacctaa caattcgttc aagccgagat ctcgagcatc agcttcaaaa
      961 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaataggt acttcaagat
     1021 ccccaattcg agctcatcgt ccgggccgca agctcctagc ggcggatttg tcctactcag
     1081 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     1141 tcgttttatt tgatgcctca agctagagag tcattacccc aggcgtttaa gggcaccaat
     1201 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtctagc ttggattctc
     1261 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1321 ggtcattact ggatctatca acaggagtcc aagctcagct aattaagcta gcttatcgat
     1381 accgtcgacc tcgaacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa
     1441 atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga
     1501 ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct
     1561 attttgttca gtttaagact ttattgtccg cccacacctg cttacgcagg gcatccattt
     1621 attactcaac cgtaaccgat tttgccaggt tacgcggctg gtcctctagc tggcgtaata
     1681 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     1741 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     1801 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     1861 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1921 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc gtcaccgaaa cgcgcgagac
     1981 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2041 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2101 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2161 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2221 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2281 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2341 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2401 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2461 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2521 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2581 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2641 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2701 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2761 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2821 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2881 ccggtgagcg tgggtcccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2941 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3001 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3181 accccgtaga aaagatcaaa ggcagttatt ggtgcctcac tgattaagca ttggtaactg
     3241 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttttg
     3301 cggccgcaag atccggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     3361 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     3421 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     3481 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     3541 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     3601 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     3661 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     3721 gctgacatgg gaattagcca tggcatcaca gtatcgtgat gacagaggca gggagtgcgg
     3781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3841 caggggattc cttaaggtat actttccgct gcataaccct gcttcggggt cattatagcg
     3901 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     3961 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     4021 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     4081 aatcctgctc tgcgaggctg gccgataagc tctgataccg ctcgccgcag ccgaacgacc
     4141 gagcgcagcg ggtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4201 cccgcgcgtt ggccgattca ttaatgcagc gcccaaacat aacaggaaga aaaatgcccc
     4261 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
     4321 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
     4381 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttct tgaggcggtt
     4441 aaaagagccg tactcttctc cgatgtcgac taggccatga tgctcattct gtgggaccaa
     4501 aacgaaaaaa caccctttcg ggtgtctttt ctggaatttg gtaccgaggc tgcaagtccc
     4561 attaaggagg cgcgccttat tacagatctt cctcagagat gagcttctgt tccagatctt
     4621 cttctgaaat caacttttgt tccagatctt cttcagagat gagtttctgc tccgcggccg
     4681 cgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg atgctctggt
     4741 ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg tcgatggtgg
     4801 tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac aggtggatga
     4861 tattctcggc ctgctctctg ataggcttgt ctctgtgctt gttgtaggcg ctcagcacct
     4921 tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc tgctcgatga
     4981 tctcgtccag gtagtgtttg tgctgttcca caaacagctg tttctgctca ttatcctcgg
     5041 gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca tatttggagg
     5101 gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt ctcttccggc
     5161 cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc tttttcactt
     5221 ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg cttctttcca
     5281 tgatggtgat ccccagcagc tctttcacac tcttcagttt cttggacttg cccttttcca
     5341 ctttggccac caccagcaca gaataggcca cggtggggct gtcgaagccg ccgtacttct
     5401 tagggtccca gtccttcttt ctggcgatca gcttgtcgct gttcctcttg ggcaggatag
     5461 actctttgct gaagccgcct gtctgcacct cggtcttttt cacgatattc acttggggca
     5521 tagacagcac tttccgcacg gtggcaaagt cccggccctt atcccacacg atctcgcctg
     5581 tttcgccgtt tgtctcgatc agaggccgct tccggatctc gccgttggcc agggtaatct
     5641 cggtcttgaa aaagttcatg atgttgctgt agaagaagta cttggcggta gccttgccga
     5701 tttcctgctc gctcttggcg atcatcttcc gcacgtcgta caccttgtag tcgccgtaca
     5761 cgaactcgct ttccagctta gggtactttt tgatcagggc ggttcccacg acggcgttca
     5821 ggtaggcgtc gtgggcgtgg tggtagttgt tgatctcgcg cactttgtaa aactggaaat
     5881 ccttccggaa atcggacacc agcttggact tcagggtgat cactttcact tcccggatca
     5941 gtttgtcgtt ctcgtcgtac ttagtgttca tccgggagtc caggatctgt gccacgtgct
     6001 ttgtgatctg ccgggtttcc accagctgtc tcttgatgaa gccggcctta tccagttcgc
     6061 tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt cctctgggta atcagcttgg
     6121 cattcagcag ctggcgccag tagttcttca tcttcttcac gacctcttcg gagggcacgt
     6181 tgtcgctctt gccccggttc ttgtcgctcc gagtcagcac tttgttatcg atggagtcgt
     6241 ccttcagaaa gctctgaggc acgatagcgt ccacatcgta gtcggacagc cggttgatgt
     6301 ccagttcctg gtccacgtac atatcccgcc cattctgcag gtagtacagg tacagcttct
     6361 cgttctgcag ctgggtgttt tccacggggt gttctttcag gatctggctg cccagctctt
     6421 tgatgccctc ttcgatccgc ttcattctct cgcggctgtt cttctgtccc ttctgggtgg
     6481 tctggttctc tctggccatt tcgatcacga tgttctcggg cttgtgccgg cccatcactt
     6541 tcacgagctc gtccaccacc ttcactgtct gcaggatgcc cttcttaatg gcggggctgc
     6601 cggccagatt ggcaatgtgc tcgtgcaggc tatcgccctg gccggacacc tgggctttct
     6661 ggatgtcctc tttaaaggtc aggctgtcgt cgtggatcag ctgcatgaag tttctgttgg
     6721 cgaagccgtc ggacttcagg aaatccagga ttgtcttgcc ggactgcttg tcccggatgc
     6781 cgttgatcag cttccggctc agcctgcccc agccggtgta tctccgccgc ttcagctgct
     6841 tcatcacttt gtcgtcgaac aggtgggcat aggttttcag ccgttcctcg atcatctctc
     6901 tgtcctcaaa cagtgtcagg gtcagcacga tatcttccag aatgtcctcg ttttcctcat
     6961 tgtccaggaa gtccttgtcc ttgataattt tcagcagatc gtggtatgtg cccagggagg
     7021 cgttgaaccg atcttccacg ccggagattt ccacggagtc gaagcactcg attttcttga
     7081 agtagtcctc tttcagctgc ttcacggtca ctttccggtt ggtcttgaac agcaggtcca
     7141 cgatggcttt tttctgctcg ccgctcagga aggcgggctt tctcattccc tcggtcacgt
     7201 atttcacttt ggtcagctcg ttgtacacgg tgaagtactc gtacagcagg ctgtgcttgg
     7261 gcagcacctt ctcgttgggc aggttcttat cgaagttggt catccgctcg atgaagctct
     7321 gggcgctggc gcccttgtcc accacttcct cgaagttcca gggggtgatg gtttcctcgc
     7381 tctttctggt catccaggcg aatctgctgt ttcccctggc cagagggccc acgtagtagg
     7441 ggatgcggaa ggtcaggatc ttctcgatct tttcccggtt gtccttcagg aatgggtaaa
     7501 aatcttcctg ccgccgcaga atggcgtgca gctctcccag gtggatctgg tgggggatgc
     7561 tgccgttgtc gaaggtccgc tgcttccgca gcaggtcctc tctgttcagc ttcacgagca
     7621 gttcctcggt gccgtccatc ttttccagga tgggcttgat gaacttgtag aactcttcct
     7681 ggctggctcc gccatcgatg tagccggcgt agccgttctt gctctggtcg aagaaaatct
     7741 ctttgtactt ctcaggcagc tgctgccgca cgagagcttt cagcagggtc aggtcctggt
     7801 ggtgctcgtc gtatctcttg atcatagagg cgctcagggg ggccttggtg atctcggtgt
     7861 tcactctcag gatgtcgctc agcaggatgg cgtcggacag gttcttggcg gccagaaaca
     7921 ggtcggcgta ctggtcgccg atctgggcca gcaggttgtc caggtcgtcg tcgtaggtgt
     7981 ccttgctcag ctgcagtttg gcatcctcgg ccaggtcgaa gttgctcttg aagttggggg
     8041 tcaggcccag gctcagggca atcaggttgc cgaacaggcc attcttcttc tcgccgggca
     8101 gctgggcgat cagattttcc agccgtctgc tcttgctcag tctggcagac aggatggcct
     8161 tggcgtccac gccgctggcg ttgatggggt tttcctcgaa cagctggttg taggtctgca
     8221 ccagctggat gaacagcttg tccacgtcgc tgttgtcggg gttcaggtcg ccctcgatca
     8281 ggaagtggcc ccggaacttg atcatgtggg ccagggccag atagatcagc cgcaggtcgg
     8341 ccttgtcggt gctgtccacc agtttctttc tcaggtggta gatggtgggg tacttctcgt
     8401 ggtaggccac ctcgtccacg atgttgccga agatggggtg ccgctcgtgc ttcttatcct
     8461 cttccaccag gaaggactct tccagtctgt ggaagaagct gtcgtccacc ttggccatct
     8521 cgttgctgaa gatctcttgc agatagcaga tccggttctt ccgtctggtg tatcttcttc
     8581 tggcggttct cttcagccgg gtggcctcgg ctgtttctcc gctgtcgaac agcagggcgc
     8641 cgatcaggtt cttcttgatg ctgtgccggt cggtgttgcc cagcaccttg aatttcttgc
     8701 tgggcacctt gtactcgtcg gtgatcacgg cccagcccac agagttggtg ccgatggcca
     8761 ggccgatgct gtacttcttg tccatactag ttttctcctc tttagatcgc tagcattgta
     8821 cctaggactg agctagccat aaagaaccga ggtaactggc ttggtgataa gctgtcaaac
     8881 cagatcaatt cgcactagtc tgcagacgta aaaaaagcgg cgtggttagc cgctttttta
     8941 attgccggag cgcaataaaa aagcccccgg aaggtgatct tccgggggct ttctcatgcg
     9001 ttgcttgtat tgaaaatggg ggccatgcct acctatctgc ctcccgggtc cttgagcccc
     9061 attatcacct ccaaagcctt ctcgtctggt cagtttcacc tgttttacgt aaaaacccgc
     9121 ttcggcgggt ttttactttt ggggaaacac agaaaaaagc ccgcacctga cagtgcgggc
     9181 tttttttttc gaccaaaggt gcgactactc ttgcctacta cctatcgact gagctgaaag
     9241 aattccggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat ccggctcgta
     9301 taattctagt tgagaccaac tttggtctcc accatagcgg tcggtctctg tttaagagct
     9361 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     9421 gcaccgagtc ggtgcttttt ttttgaattc atgtggctga ccgttctgtt gtctctcgct
     9481 cttccgagta gacgaacaat aaggcctccc taacgggggg ccttttttat tgataacaaa
     9541 agtcagtgct tccgctattt ccaaaatacc gggctaatac ggtttaaacg acctcctgga
     9601 tttgctcaga cagccttttc gtcattcgtt tcagccaaaa aacttaagac cgccggtctt
     9661 gtccactacc ttgcagtaat gcggtggaca ggatcggcgg ttttcttttc tctt
//
