LOCUS       sequence-269564-        9714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Promoter 3 with bsa1 cut sites for inserting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Qu J, Prasad NK, Yu MA, Chen S, Lyden A, Herrera N, Silvis MR, 
            Crawford E, Looney MR, Peters JM, Rosenberg OS
  TITLE     Modulating pathogenesis with Mobile-CRISPRi.
  JOURNAL   J Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19.
  PUBMED    31481541
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19."
COMMENT     Sequence Label: PJQ49
FEATURES             Location/Qualifiers
     source          1..9714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(367..372,373..389,390..395))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    512..559
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(join(975..980,981..997,998..1003))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        1436..1470
                     /label=BBa_J23115
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.15"
     RBS             1476..1487
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1494..1520,1521..1523,1524..4010,4011..4013,
                     4014..5597)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1494..5597
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5607..5696
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     mobile_element  5851..6016
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            6175..6283
                     /note="incP origin of transfer"
     rep_origin      6552..6940
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(7241..8032,8033..8101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7864..7883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8102..8206)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8274..8292
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8330..8352)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8452..8471
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8885..8901
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8886..8902
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      8930..9024
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      9127..9213
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    9264..9311
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(join(9359..9714,1..178))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
       61 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
      121 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
      181 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
      241 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      301 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
      361 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
      421 cacggtgtgc gtccatgggc aaatattata cgcaaggcga caaggctcga gagttcaaca
      481 gacagccttc gttcttatgg ccgttcagga ggaagttcct attccgaagt tcctattctc
      541 tagaaagtat aggaacttct tgagaagaga aaagaaaacc gccgatcctg tccaccgcat
      601 tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa cgaatgacga
      661 aaaggctgtc tgagcaaatc caggaggtcg tttaaaccgt attagcccgg tattttggaa
      721 atagcggaag cactgacttt tgttatcaat aaaaaaggcc ccccgttagg gaggccttat
      781 tgttcgtcta ctcggaagag cgagagacaa cagaacggtc agccacatga attcaaaaaa
      841 aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta tttaaacttg
      901 ctatgctgtt tccagcatag ctcttaaaca gagaccgacc gctatggtgg agaccaaagt
      961 tggtctcaac tagaattata cgagccggat gattaattgt caacagctca tttcagaata
     1021 tttgccagaa ccggaattct ttcagctcag tcgataggta gtaggcaaga gtagtcgcac
     1081 ctttggtcga aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttccccaa
     1141 aagtaaaaac ccgccgaagc gggtttttac gtaaaacagg tgaaactgac cagacgagaa
     1201 ggctttggag gtgataatgg ggctcaagga cccgggaggc agataggtag gcatggcccc
     1261 cattttcaat acaagcaacg catgagaaag cccccggaag atcaccttcc gggggctttt
     1321 ttattgcgct ccggcaatta aaaaagcggc taaccacgcc gcttttttta cgtctgcaga
     1381 ctagtgcgaa ttgatctggt ttgacagctt atcaccaagc cagttacctc ggttctttat
     1441 agctagctca gcccttggta caatgctagc gatctaaaga ggagaaaact agtatggaca
     1501 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg
     1561 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     1621 tcaagaagaa cctgatcggc gccctgctgt tcgacagcgg agaaacagcc gaggccaccc
     1681 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     1741 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     1801 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     1861 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     1921 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     1981 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     2041 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     2101 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     2161 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg
     2221 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     2281 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     2341 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     2401 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     2461 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     2521 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     2581 tcgatggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     2641 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     2701 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     2761 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     2821 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     2881 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     2941 acaagggcgc cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     3001 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg
     3061 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     3121 agcagaaaaa agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     3181 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     3241 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     3301 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     3361 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     3421 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     3481 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     3541 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     3601 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     3661 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     3721 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     3781 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     3841 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     3901 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     3961 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     4021 ctcagagctt tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga
     4081 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     4141 ggcgccagct gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg
     4201 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     4261 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     4321 acgacgagaa cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     4381 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     4441 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     4501 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     4561 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     4621 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     4681 tcgagacaaa cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg
     4741 tgcggaaagt gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     4801 gcggcttcag caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa
     4861 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     4921 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     4981 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     5041 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     5101 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     5161 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     5221 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     5281 aacactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     5341 ccgacgctaa tctggacaag gtgctgagcg cctacaacaa gcacagagac aagcctatca
     5401 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     5461 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     5521 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     5581 ctcagctggg aggcgacgcg gccgcggagc agaaactcat ctctgaagaa gatctggaac
     5641 aaaagttgat ttcagaagaa gatctggaac agaagctcat ctctgaggaa gatctgtaat
     5701 aaggcgcgcc tccttaatgg gacttgcagc ctcggtacca aattccagaa aagacacccg
     5761 aaagggtgtt ttttcgtttt ggtcccacag aatgagcatc atggcctagt cgacatcgga
     5821 gaagagtacg gctcttttaa ccgcctcaag aaccagataa gtgaaatcta gttccaaact
     5881 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     5941 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     6001 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttgggcgc tgcattaatg
     6061 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6121 cactgacccg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagagc ttatcggcca
     6181 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     6241 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     6301 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     6361 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaagtat
     6421 accttaagga atcccctgat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6481 ccaggaaccg taaaaaggcc gcactccctg cctctgtcat cacgatactg tgatgccatg
     6541 gctaattccc atgtcagccg ttaagtgttc ctgtgtcact caaaattgct ttgagaggct
     6601 ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc cacaaccgtt aaaccttaaa
     6661 agctttaaaa gccttatata ttcttttttt tcttataaaa cttaaaacct tagaggctat
     6721 ttaagttgct gatttatatt aattttattg ttcaaacatg agagcttagt acgtgaaaca
     6781 tgagagctta gtacgttagc catgagagct tagtacgtta gccatgaggg tttagttcgt
     6841 taaacatgag agcttagtac gttaaacatg agagcttagt acgtgaaaca tgagagctta
     6901 gtacgtacta tcaacaggtt gaactgctga tcttcagatc ctctacgccg gacgcatcgt
     6961 ggccggatct tgcggccgca aaaattaaaa atgaagtttt aaatcaatct aaagtatata
     7021 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccaa taactgcctt
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7381 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8221 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8281 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgacga cggtgaaaac ctctgacaca
     8341 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8401 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     8461 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8521 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     8581 cggtgcgggc ctcttcgcta ttacgccagc tagaggacca gccgcgtaac ctggcaaaat
     8641 cggttacggt tgagtaataa atggatgccc tgcgtaagca ggtgtgggcg gacaataaag
     8701 tcttaaactg aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata
     8761 gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg
     8821 ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg
     8881 gggttcgagg tcgacggtat cgataagcta gcttaattag ctgagcttgg actcctgttg
     8941 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     9001 cgggcgtttt ttattggtga gaatccaagc tagactgcga tgagtggcag ggcggggcgt
     9061 aattttttta aggcagttat tggtgccctt aaacgcctgg ggtaatgact ctctagcttg
     9121 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     9181 ttgtcggtga acgctctcct gagtaggaca aatccgccgc taggagcttg cggcccggac
     9241 gatgagctcg aattggggat cttgaagtac ctattccgaa gttcctattc tctagaaagt
     9301 ataggaactt cagagcgctt ttgaagctga tgctcgagat ctcggcttga acgaattgtt
     9361 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     9421 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     9481 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     9541 ggtgctctcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
     9601 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     9661 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgat
//
