LOCUS       sequence-269571-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1058 encoding truncated human cytosine deaminase, hAIDx, at the 
            C-terminus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: 1058-AIDx-CP
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..48)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        220..599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        600..803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     754..774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     848..867
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        848..866
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5081..5101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5117..5137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5759..5806
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5807..5830
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5840..5896
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6389..6406)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6395..6619
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6665..7093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6752..6771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6962..6983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7102..7122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7107..7436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7287..7422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7349..7368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7503..8297
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7557..7576)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8167..8186
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8471..8592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8508..8527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8562..8581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8641..8657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8641..8657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8654..8676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8665..8681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8689..8695,8696..8713,8714..8719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8734..8755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8872..8889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9043..9631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9123..9142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9802..10593,10594..10662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10425..10444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10663..10767)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
       61 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
      121 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
      181 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      241 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      301 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      361 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      421 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      481 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      541 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      601 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      661 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      721 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      781 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      841 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
      901 tggtaccgcc accatgaatc gtaaacgccc gctgattgaa accaacggtg aaaccggcga
      961 aatcgtgtgg gacaagggcc gtgattttgc gaccgtgcgc aaagtgctgt ccatgccgca
     1021 ggtgaacatt gtgaagaaaa ccgaggtgca gaccggcggt ttcagcaagg aaagcattct
     1081 gccgaaacgt aacagcgaca agctgatcgc ccgcaagaaa gattgggacc cgaagaaata
     1141 cggcggtttc gatagcccga ccgtggctta tagcgtgctg gtggtggcca aagtggagaa
     1201 gggtaaaagc aagaaactga agagcgtgaa agaactgctg ggcatcacca ttatggagcg
     1261 tagcagcttc gaaaagaacc cgatcgactt tctggaggcg aagggttaca aggaagtgaa
     1321 gaaagatctg atcattaagc tgccgaaata cagcctgttt gagctggaaa atggtcgtaa
     1381 acgtatgctg gccagcgccg gcgagctgca aaagggcaac gaactggcgc tgccgagcaa
     1441 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaaaggta gcccggagga
     1501 caacgaacag aagcagctgt ttgtggagca gcacaaacac tatctggatg agatcattga
     1561 acagatcagc gagttcagca agcgtgtgat tctggcggac gctaacctgg ataaagtgct
     1621 gagcgcctac aacaaacacc gtgacaagcc gatccgcgag caggcggaaa acatcattca
     1681 cctgttcacc ctgaccaacc tgggtgcgcc ggccgcgttc aagtactttg acaccaccat
     1741 tgatcgtaaa cgctatacca gcaccaagga ggtgctggat gctaccctga tccaccagag
     1801 cattaccggc ctgtacgaaa cccgtattga tctgagccag ctgggcggtg acggcggtag
     1861 cggcggtagc ggcggtagcg gcggtagcgg cggtagcggc ggtagcggcg gtgacaagaa
     1921 atatagcatc ggtctggcga ttggcaccaa cagcgtgggt tgggctgtga tcaccgatga
     1981 gtacaaggtg ccgagcaaga agttcaaggt gctgggcaac accgaccgcc acagcatcaa
     2041 gaaaaacctg attggtgccc tgctgtttga tagcggtgaa accgccgagg ccacccgtct
     2101 gaagcgcacc gcccgtcgcc gttacacccg tcgtaaaaac cgtatctgct acctgcaaga
     2161 gattttcagc aacgaaatgg ccaaagtgga cgatagcttc tttcaccgcc tggaggagag
     2221 cttcctggtg gaggaagaca agaaacacga gcgtcacccg atctttggca acattgtgga
     2281 tgaggtggct taccacgaaa agtatccgac catctaccac ctgcgtaaga aactggtgga
     2341 tagcaccgac aaggccgatc tgcgcctgat ctatctggct ctggcccaca tgatcaagtt
     2401 ccgtggtcac tttctgatcg agggcgacct gaacccggat aacagcgatg tggacaagct
     2461 gttcatccag ctggtgcaga cctacaacca gctgtttgag gaaaacccga ttaacgccag
     2521 cggtgtggac gcgaaggcta tcctgagcgc gcgtctgagc aaaagccgcc gtctggagaa
     2581 cctgatcgcg cagctgccgg gtgaaaagaa aaacggtctg tttggcaacc tgattgctct
     2641 gagcctgggc ctgaccccga acttcaaaag caactttgac ctggcggagg atgctaagct
     2701 gcaactgagc aaggatacct atgacgatga cctggacaac ctgctggcgc agatcggcga
     2761 ccagtacgct gatctgttcc tggctgccaa gaacctgagc gatgcgatcc tgctgagcga
     2821 cattctgcgt gtgaacaccg agattaccaa ggccccgctg agcgcgagca tgatcaaacg
     2881 ctatgatgaa caccaccagg acctgaccct gctgaaagct ctggtgcgtc agcagctgcc
     2941 ggagaagtac aaagaaatct tctttgacca gagcaagaac ggttacgccg gctatattga
     3001 tggcggtgcg agccaggagg agttctacaa gtttatcaaa ccgattctgg agaaaatgga
     3061 cggcaccgag gaactgctgg tgaagctgaa ccgtgaagat ctgctgcgta aacagcgcac
     3121 cttcgacaac ggtagcatcc cgcaccagat tcacctgggt gagctgcacg ccatcctgcg
     3181 ccgtcaggaa gatttctacc cgtttctgaa ggacaaccgt gagaagatcg aaaaaattct
     3241 gaccttccgc attccgtact atgtgggtcc gctggctcgt ggcaacagcc gctttgcctg
     3301 gatgacccgc aagagcgagg aaaccatcac cccgtggaac ttcgaggaag tggtggacaa
     3361 aggcgccagc gcgcagagct tcattgagcg tatgaccaac tttgataaga acctgccgaa
     3421 cgaaaaagtg ctgccgaagc acagcctgct gtacgagtat ttcaccgtgt acaacgaact
     3481 gaccaaggtg aaatatgtga ccgagggtat gcgcaagccg gccttcctga gcggcgaaca
     3541 gaagaaagcg atcgtggacc tgctgtttaa aaccaaccgt aaggtgaccg tgaagcagct
     3601 gaaagaggat tatttcaaga aaattgaatg ctttgacagc gtggagatca gcggtgtgga
     3661 agatcgtttt aacgcgagcc tgggcaccta ccacgacctg ctgaaaatca ttaaggataa
     3721 agacttcctg gataacgagg aaaacgagga catcctggaa gatattgtgc tgaccctgac
     3781 cctgtttgag gaccgtgaaa tgatcgagga acgcctgaag acctatgccc acctgttcga
     3841 tgacaaagtg atgaaacagc tgaagcgccg tcgctacacc ggttggggcc gtctgagccg
     3901 caaactgatc aacggtattc gcgataaaca gagcggcaag accatcctgg attttctgaa
     3961 gagcgacggt ttcgcgaacc gtaactttat gcagctgatt cacgatgaca gcctgacctt
     4021 caaagaggac atccagaagg cgcaggtgag cggtcagggc gatagcctgc acgaacatat
     4081 tgctaacctg gccggtagcc cggccatcaa gaagggcatc ctgcaaaccg tgaaggtggt
     4141 ggacgagctg gtgaaagtga tgggtcgtca caagccggaa aacatcgtga ttgagatggc
     4201 ccgcgaaaac cagaccaccc agaaaggcca gaagaacagc cgtgagcgca tgaagcgtat
     4261 cgaggaaggc atcaaggaac tgggcagcca gatcctgaag gagcacccgg tggaaaacac
     4321 ccagctgcaa aacgagaaac tgtacctgta ctacctgcaa aacggtcgtg acatgtatgt
     4381 ggatcaggaa ctggacatta accgcctgag cgattatgac gtggatgcga tcgtgccgca
     4441 gagcttcctg aaggatgaca gcattgacaa caaagtgctg acccgtagcg ataaaaaccg
     4501 cggcaagagc gacaacgtgc cgagcgagga agtggtgaag aaaatgaaaa actactggcg
     4561 tcagctgctg aacgccaaac tgatcaccca gcgtaagttt gacaacctga ccaaagctga
     4621 gcgcggcggt ctgagcgaac tggataaagc cggtttcatt aagcgtcagc tggtggaaac
     4681 ccgccagatc accaagcacg tggcgcagat tctggacagc cgcatgaaca ccaagtatga
     4741 cgagaacgat aaactgatcc gtgaagtgaa agtgatcacc ctgaagagca aactggtgag
     4801 cgattttcgt aaagacttcc agttttacaa ggtgcgcgag atcaacaact accaccacgc
     4861 tcacgacgcc tatctgaacg cggtggtggg caccgccctg atcaagaagt acccgaagct
     4921 ggagagcgag ttcgtgtacg gcgactataa agtgtatgat gttcgtaaga tgatcgcaaa
     4981 gtccgagcag gagattggca aggcaaccgc taagtatttt ttttacagca acatcatgaa
     5041 cttctttaag accgagatta ccctggccaa cggtgaaatc ccgaagaaaa agcgtaaagt
     5101 gggtagcagc ggcagcccga aaaagaaacg caaagtgggt agcgacgctc tggatgactt
     5161 cgacctggat atgctgggca gcgacgccct ggatgacttt ggcggtggca gcatggatag
     5221 cctgctgatg aaccgtcgca aattcctgta tcagtttaag aacgtgcgtt gggctaaagg
     5281 ccgtcgcgaa acctacctgt gctatgtggt gaaacgtcgc gatagcgcca ccagcttcag
     5341 cctggacttt ggttacctgc gcaacaagaa cggctgccac gtggagctgc tgttcctgcg
     5401 ttacattagc gactgggacc tggacccagg ccgttgctat cgcgtgacct ggtttaccag
     5461 ctggagcccg tgctacgatt gcgctcgtca tgtggcggac ttcctgcgtg gtaacccaaa
     5521 cctgagcctg cgtatcttca ccgcgcgcct gtacttttgc gaggaccgta aggctgagcc
     5581 ggaaggtctg cgtcgcctgc accgtgcggg tgtgcagatc gcgatcatga ccttcaaaga
     5641 ttacttttat tgctggaaca ccttcgtgga gaaccacgaa cgcaccttta aggcctggga
     5701 gggtctgcac gaaaacagcg tgcgtctgag ccgccagctg cgccgcatcc tgctgccaaa
     5761 gcgtccggcg gctaccaaga aagcgggcca ggctaagaaa aagaaagact acaaagatga
     5821 cgatgacaag ggtagcggcg ctaccaactt cagcctgctg aagcaggcgg gcgacgtgga
     5881 ggaaaatccg ggcccgatgg ctaaaccgct gagccaggag gaaagcaccc tgatcgagcg
     5941 cgctaccgcc accattaaca gcatcccgat tagcgaagac tatagcgtgg ccagcgccgc
     6001 gctgagcagc gatggtcgta ttttcaccgg cgtgaacgtg taccacttta ccggtggccc
     6061 gtgcgccgag ctggtggtgc tgggcaccgc cgccgccgcc gccgccggca acctgacctg
     6121 catcgtggcg attggtaacg aaaatcgtgg catcctgagc ccgtgcggcc gttgccgcca
     6181 ggtgctgctg gacctgcatc cgggcatcaa ggcgattgtg aaggatagcg acggccaacc
     6241 gaccgccgtg ggtattcgtg aactgctgcc atccggctat gtttgggagg gctaaggatc
     6301 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
     6361 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6421 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6481 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6541 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6601 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6661 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6961 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7021 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7081 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7201 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7261 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7321 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7381 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7441 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     7501 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7561 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7621 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7681 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7741 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7801 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7861 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7921 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7981 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     8041 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8101 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8161 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8221 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8281 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     8341 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8401 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8461 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8521 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8581 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8641 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8701 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8761 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8821 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8881 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8941 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9001 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9061 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10801 cggatcggga gat
//
