LOCUS       sequence-269594-       11140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HyPr plasmid with natMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Peris D, Alexander WG, Fisher KJ, Moriarty RV, Basuino MG, Ubbelohde
            EJ, Wrobel RL, Hittinger CT
  TITLE     Synthetic hybrids of six yeast species.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2085. doi: 10.1038/s41467-020-15559-4.
  PUBMED    32350251
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2085. doi"; pages: " 
            10.1038/s41467-020-15559-4"
COMMENT     Sequence Label: pHCT2
FEATURES             Location/Qualifiers
     source          1..11140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             1611..1622
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2268..2765
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     CDS             2385..2882
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      2958..3205
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2974..2992)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3228..3244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3228..3244)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3241..3263)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3252..3268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3276..3282,3283..3300,3301..3306))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3321..3342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3459..3476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3630..4218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3710..3729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4389..5180,5181..5249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5012..5031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5250..5354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5422..5440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            5562..6681
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        5562..5905
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5906..6478
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      6484..6681
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      7024..7861
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     misc_feature    7997..8289
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     primer_bind     9031..9053
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9045..9062
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9046..9062
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(9090..9476,9477..10097))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     10005..10024
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(10182..10206)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(10203..10406)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10232..10252)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10407..10786
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(10951..10975)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtaccctatg
      301 gcatgcatgt gctctgtatg tatataaaac tcttgttttc ttcttttctc taaatattct
      361 ttccttatac attaggtcct ttgtagcata aattactata cttctataga cacgcaaaca
      421 caaatacaca cactaaatta ccggatcaat tcgggggaaa aatgctttct gaaaacacga
      481 ctattctgat ggctaacggt gaaattaaag acatcgcaaa cgtcacggct aactcttacg
      541 ttatgtgcgc agatggctcc gctgcccgcg tcataaatgt cacacagggc tatcagaaaa
      601 tctataatat acagcaaaaa accaaacaca gagcttttga aggtgaacct ggtaggttag
      661 atcccaggcg tagaacagtt tatcagcgtc ttgcattaca atgtactgca ggtcataaat
      721 tgtcagtcag ggtccctacc aaaccactgt tggaaaaaag tggtagaaat gccaccaaat
      781 ataaagtgag atggagaaat ctgcagcaat gtcagacgct tgatggtagg ataataataa
      841 ttccaaaaaa ccatcataag acattcccaa tgacagttga aggtgagttt gccgcaaaac
      901 gcttcataga agaaatggag cgctctaaag gagaatattt caactttgac attgaagtta
      961 gagatttgga ttatcttgat gctcaattga gaatttctag ctgcataaga tttggtccag
     1021 tactcacagg aaatggtgtt ttatctaaat ttctcactgg acgtagtgac cttgtaactc
     1081 ctgctgtaaa aagtatggct tggatgcttg gtctgtggtt aggtgacggt acaacaaaag
     1141 agccagaaat ctcagtagat agcttggatc ctaagctaat ggagagttta agagaaaatg
     1201 cgaaaatctg gggtctctac cttacggttt gtgacgatca cgttccgcta cgtgccaaac
     1261 atgtaaggct tcattatgga gatggtccag atgaaaacag gaagacaagg aatttgagga
     1321 aaaataatcc attctggaaa gctgtcacaa ttttaaagtt taaaagggat cttgatggag
     1381 agaagcaaat ccctgaattt atgtacggcg agcatataga agttcgtgaa gcattcttag
     1441 ccggcttgat cgactcagat gggtacgttg tgaaaaaggg cgaaggccct gaatcttata
     1501 aaatagcaat tcaaactgtt tattcatcca ttatggacgg aattgtccat atttcaagat
     1561 ctcttggtat gtcagctact gtgacgacca ggtcagctag ggaggaaatc attgaaggaa
     1621 gaaaagtcca atgtcaattt acatacgact gtaatgttgc tgggggaaca actttacaga
     1681 atgttttgtc atattgtcga agtggtcaca aaacaagaga agttccgcca attataaaaa
     1741 gggaacccgt atatttcagc ttcacggatg atttccaggg tgagagtact gtatatgggc
     1801 ttacgataga aggccataaa aatttcttgc ttggcaacaa aatagaagtg aaatcatgtc
     1861 gaggctgctg tgtgggagaa cagcataaaa tatcacaaaa aaagaatcta aaacactgtg
     1921 ttgcttgtcc cagaaaggga atcaagtatt tttataaaga ttggagtggt aaaaatcgag
     1981 tatgtgctag atgctatgga agatacaaat tcagcggtca tcactgtata aattgcaagt
     2041 atgtaccaga agcacgtgaa gtgaaaaagg caaaagacaa aggcgaaaaa ttgggcatta
     2101 cgcccgaagg tttgccagtt aaaggaccag agtgtataaa atgtggcgga atcttacagt
     2161 ttgatgctgt ccgcgggcct cataagagtt gtggtaacaa cgcaggtgcg cgcatctgcc
     2221 ggatccccgg gttaattaac ggtggaggtg gaggtggagg tggaggtgaa caaaagctaa
     2281 tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac ttgaacggac
     2341 tcgacggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct
     2401 ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggactcg
     2461 acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg
     2521 aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac ggactcgacg
     2581 gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg
     2641 aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga ctcgacggtg
     2701 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     2761 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc gacggtgaac
     2821 aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact
     2881 tgaacggtga acaaaaatta atcaatcact agtgaattcg cgccacttct aaataagcga
     2941 atttcttgag ggccgcatca tgtaattagt tatgtcacgc ttacattcac gccctccccc
     3001 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     3061 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     3121 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
     3181 gggacgctcg aaggctttaa tttgcggcca agcttggcgt aatcatggtc atagctgttt
     3241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5341 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     5401 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc
     5461 agctgaagct tcgtacgctg caggtcgacg gatccccggg ttaattaagg cgcgccagat
     5521 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
     5581 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
     5641 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
     5701 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
     5761 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
     5821 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
     5881 acatcacatc cgaacataaa caaccatggg taccactctt gacgacacgg cttaccggta
     5941 ccgcaccagt gtcccggggg acgccgaggc catcgaggca ctggatgggt ccttcaccac
     6001 cgacaccgtc ttccgcgtca ccgccaccgg ggacggcttc accctgcggg aggtgccggt
     6061 ggacccgccc ctgaccaagg tgttccccga cgacgaatcg gacgacgaat cggacgacgg
     6121 ggaggacggc gacccggact cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc
     6181 gggcttcgtg gtcgtctcgt actccggctg gaaccgccgg ctgaccgtcg aggacatcga
     6241 ggtcgccccg gagcaccggg ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga
     6301 gttcgcccgc gagcggggcg ccgggcacct ctggctggag gtcaccaacg tcaacgcacc
     6361 ggcgatccac gcgtaccggc ggatggggtt caccctctgc ggcctggaca ccgccctgta
     6421 cgacggcacc gcctcggacg gcgagcaggc gctctacatg agcatgccct gcccctaatc
     6481 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     6541 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     6601 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     6661 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa
     6721 acgagctcga attcatcgat gatatcagat ccactagtgg cctatgcaat tctcatgttt
     6781 gacagcttat catcgatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag
     6841 ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc
     6901 agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg
     6961 acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc
     7021 gagagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt
     7081 agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa
     7141 gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag
     7201 tatttattta agtattgttt gtgcacttgc ctgcaagcct tttgaaaagc aagcataaaa
     7261 gatctaaaca taaaatctgt aaaataacaa gatgtaaaga taatgctaaa tcatttggct
     7321 ttttgattga ttgtacagga aaatatacat cgcagggggt tgacttttac catttcaccg
     7381 caatggaatc aaacttgttg aagagaatgt tcacaggcgc atacgctaca atgacccgat
     7441 tcttgctagc cttttctcgg tcttgcaaac aaccgccggc agcttagtat ataaatacac
     7501 atgtacatac ctctctccgt atcctcgtaa tcattttctt gtatttatcg tcttttcgct
     7561 gtaaaaactt tatcacactt atctcaaata cacttattaa ccgcttttac tattatcttc
     7621 tacgctgaca gtaatatcaa acagtgacac atattaaaca cagtggtttc tttgcataaa
     7681 caccatcagc ctcaagtcgt caagtaaaga tttcgtgttc atgcagatag ataacaatct
     7741 atatgttgat aattagcgtt gcctcatcaa tgcgagatcc gtttaaccgg accctagtgc
     7801 acttacccca cgttcggtcc actgtgtgcc gaacatgctc cttcactatt ttaacatgtg
     7861 gaattatcga tgccggtgtt attcttgcta tgataggcca tcgaatgtta aagggaagat
     7921 ttattgtttt gccctttaaa gacgaatttg gaccgtgaat gtttaaataa ttctcatgtt
     7981 tgacagctta tcatcgaact ctaagaggtg atacttattt actgtaaaac tgtgacgata
     8041 aaaccggaag gaagaataag aaaactcgaa ctgatctata atgcctattt tctgtaaaga
     8101 gtttaagcta tgaaagcctc ggcattttgg ccgctcctag gtagtgcttt ttttccaagg
     8161 acaaaacagt ttctttttct tgagcaggtt ttatgtttcg gtaatcataa acaataaata
     8221 aattatttca tttatgttta aaaataaaaa ataaaaaagt attttaaatt tttaaaaaag
     8281 ttgattataa gcatgtgacc ttttgcaagc aattaaattt tgcaatttgt gatttaggca
     8341 aaagttacta tttctggctc gtgtaatata tgtatgctaa tgtgaacttt tacaaagtcg
     8401 atatggactt agtcaaaaga aattttctta aaaatatata gcactagcca atttagcact
     8461 tctttatgag atatattata gactttatta agccagattt gtgtattata tgtatttacc
     8521 cggcgaatca tggacataca ttctgaaata ggtaatattc tctatggtga gacagcatag
     8581 ataacctagg atacaagtta aaagctagta ctgttttgca gtaatttttt tcttttttat
     8641 aagaatgtta ccacctaaat aagttataaa gtcaatagtt aagtttgata tttgattgta
     8701 aaataccgta atatatttgc atgatcaaaa ggctcaatgt tgactagcca gcatgtcaac
     8761 cactatattg atcaccgata ttaggacttc cacaccaact agtaatatga caataaattc
     8821 aagatattct tcatgagaat ggcccagcga ttatgcggtg tgaaataccg cacagatgcg
     8881 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     8941 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9001 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     9061 gtgaattctt attacgatcc tcgcgccccc tacccaccgt actcgtcaat tccaagggca
     9121 tcggtaaaca tctgctcaaa ctcgaagtcg gccatatcca gagcgccgta gggggcggag
     9181 tcgtgggggg taaatcccgg acccggggaa tccccgtccc ccaacatgtc cagatcgaaa
     9241 tcgtctagcg cgtcggcatg cgccatcgcc acgtcctcgc cgtctaagtg gagctcgtcc
     9301 cccaggctga catcggtcgg gggggccgtc gacagtctgc gcgtgtgtcc cgcggggaga
     9361 aaggacaggc gcggagccgc cagccccgcc tcttcggggg cgtcgtcgtc cgggagatcg
     9421 agcaggccct cgatggtaga cccgtaattg tttttcgtac gcgcgcggct gtacgcggac
     9481 ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag
     9541 aaggctggct ctgcaccttg gcgatcaaat aattcgatag cttgtcgtaa taatggcggc
     9601 atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgttcttcc
     9661 aatacgcaac ctaaagtaaa gtgccccaca gcgctgagtg catataatgc attctctagt
     9721 gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct
     9781 gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta
     9841 aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag
     9901 tgagtatggt gcctatctaa catctcaatg ggtaaggcgt cgagcaaagc ccgcttattt
     9961 tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt
    10021 gttaaacctt cgattccgac cccattaagc agctctaatg cgccgttaat cactttactt
    10081 ttatctaatc tagacatatg aattaattcg gggccgcgga ggctggatcg gtcccggtgt
    10141 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa
    10201 cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
    10261 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
    10321 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
    10381 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
    10441 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
    10501 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
    10561 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
    10621 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
    10681 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
    10741 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaaca tggcggtaat
    10801 gttggacatg agccaatata aatgtacata ttatgatatg gatacaacgt atgcaatggg
    10861 ccaagctcct cgagtaattc gcgccacttc taataagcga tttcttatga tttatgattt
    10921 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
    10981 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
    11041 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
    11101 acagggtaat atagatcaat tcctcgatcg agtttaccac
//
