LOCUS       sequence-269595-       10858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HyPr plasmid with ble marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Peris D, Alexander WG, Fisher KJ, Moriarty RV, Basuino MG, Ubbelohde
            EJ, Wrobel RL, Hittinger CT
  TITLE     Synthetic hybrids of six yeast species.
  JOURNAL   Nat Commun. 2020 Apr 29;11(1):2085. doi: 10.1038/s41467-020-15559-4.
  PUBMED    32350251
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-29"; volume: "11(1):2085. doi"; pages: " 
            10.1038/s41467-020-15559-4"
COMMENT     Sequence Label: pHRW32
FEATURES             Location/Qualifiers
     source          1..10858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1265..1276
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1922..2419
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     CDS             2039..2536
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      2612..2859
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2628..2646)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2882..2898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2882..2898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2895..2917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2906..2922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2930..2936,2937..2954,2955..2960))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2975..2996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3113..3130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3284..3872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3364..3383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4043..4834,4835..4903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4666..4685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4904..5008)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5076..5094
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5216..5559
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      5940..6137
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      6480..7317
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     misc_feature    7453..7745
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     primer_bind     8487..8509
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8501..8518
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8502..8518
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(8546..8932,8933..9553))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     9461..9480
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(9638..9662)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9659..9862)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9688..9708)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9863..10242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(10407..10431)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    10597..10615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10639..10657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10681..10699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10723..10741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10765..10783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tctctaaata ttctttcctt atacattagg tcctttgtag cataaattac tatacttcta
       61 tagacacgca aacacaaata cacacactaa attaccggat caattcgggg gaaaaatgct
      121 ttctgaaaac acgactattc tgatggctaa cggtgaaatt aaagacatcg caaacgtcac
      181 ggctaactct tacgttatgt gcgcagatgg ctccgctgcc cgcgtcataa atgtcacaca
      241 gggctatcag aaaatctata atatacagca aaaaaccaaa cacagagctt ttgaaggtga
      301 acctggtagg ttagatccca ggcgtagaac agtttatcag cgtcttgcat tacaatgtac
      361 tgcaggtcat aaattgtcag tcagggtccc taccaaacca ctgttggaaa aaagtggtag
      421 aaatgccacc aaatataaag tgagatggag aaatctgcag caatgtcaga cgcttgatgg
      481 taggataata ataattccaa aaaaccatca taagacattc ccaatgacag ttgaaggtga
      541 gtttgccgca aaacgcttca tagaagaaat ggagcgctct aaaggagaat atttcaactt
      601 tgacattgaa gttagagatt tggattatct tgatgctcaa ttgagaattt ctagctgcat
      661 aagatttggt ccagtactca caggaaatgg tgttttatct aaatttctca ctggacgtag
      721 tgaccttgta actcctgctg taaaaagtat ggcttggatg cttggtctgt ggttaggtga
      781 cggtacaaca aaagagccag aaatctcagt agatagcttg gatcctaagc taatggagag
      841 tttaagagaa aatgcgaaaa tctggggtct ctaccttacg gtttgtgacg atcacgttcc
      901 gctacgtgcc aaacatgtaa ggcttcatta tggagatggt ccagatgaaa acaggaagac
      961 aaggaatttg aggaaaaata atccattctg gaaagctgtc acaattttaa agtttaaaag
     1021 ggatcttgat ggagagaagc aaatccctga atttatgtac ggcgagcata tagaagttcg
     1081 tgaagcattc ttagccggct tgatcgactc agatgggtac gttgtgaaaa agggcgaagg
     1141 ccctgaatct tataaaatag caattcaaac tgtttattca tccattatgg acggaattgt
     1201 ccatatttca agatctcttg gtatgtcagc tactgtgacg accaggtcag ctagggagga
     1261 aatcattgaa ggaagaaaag tccaatgtca atttacatac gactgtaatg ttgctggggg
     1321 aacaacttta cagaatgttt tgtcatattg tcgaagtggt cacaaaacaa gagaagttcc
     1381 gccaattata aaaagggaac ccgtatattt cagcttcacg gatgatttcc agggtgagag
     1441 tactgtatat gggcttacga tagaaggcca taaaaatttc ttgcttggca acaaaataga
     1501 agtgaaatca tgtcgaggct gctgtgtggg agaacagcat aaaatatcac aaaaaaagaa
     1561 tctaaaacac tgtgttgctt gtcccagaaa gggaatcaag tatttttata aagattggag
     1621 tggtaaaaat cgagtatgtg ctagatgcta tggaagatac aaattcagcg gtcatcactg
     1681 tataaattgc aagtatgtac cagaagcacg tgaagtgaaa aaggcaaaag acaaaggcga
     1741 aaaattgggc attacgcccg aaggtttgcc agttaaagga ccagagtgta taaaatgtgg
     1801 cggaatctta cagtttgatg ctgtccgcgg gcctcataag agttgtggta acaacgcagg
     1861 tgcgcgcatc tgccggatcc ccgggttaat taacggtgga ggtggaggtg gaggtggagg
     1921 tgaacaaaag ctaatctccg aggaagactt gaacggtgaa caaaaattaa tctcagaaga
     1981 agacttgaac ggactcgacg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga
     2041 acaaaagcta atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga
     2101 cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca
     2161 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
     2221 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     2281 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     2341 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     2401 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
     2461 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
     2521 ctccgaggaa gacttgaacg gtgaacaaaa attaatcaat cactagtgaa ttcgcgccac
     2581 ttctaaataa gcgaatttct tgagggccgc atcatgtaat tagttatgtc acgcttacat
     2641 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     2701 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     2761 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     2821 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccaagcttg gcgtaatcat
     2881 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2941 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3001 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3061 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4141 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     5041 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     5101 gtcttcaaga attcagctga agcttcgtac gctgcaggtc gacggatccc cgggttaatt
     5161 aaggcgcgcc agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     5221 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     5281 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     5341 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     5401 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     5461 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     5521 aggatacagt tctcacatca catccgaaca taaacaacca tggctaagtt gacctctgct
     5581 gttccagttt tgactgctag agatgttgct ggtgctgttg aattttggac cgacagattg
     5641 ggtttttcca gagatttcgt tgaagacgat ttcgctggtg ttgttcgtga cgatgttacc
     5701 ttgttcatct ccgccgttca agatcaagtt gttccagata atactttggc ttgggtttgg
     5761 gttagaggtt tagacgagtt gtatgctgaa tggtctgaag tcgtttctac taacttcaga
     5821 gatgcttctg gtccagctat gaccgaaatt ggtgaacaac catggggtag agaatttgcc
     5881 ttgagagacc cagccggtaa ttgtgttcat ttcgtcgccg aagaacaaga ttgatcagta
     5941 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     6001 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     6061 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     6121 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga
     6181 gctcgaattc atcgatgata tcagatccac tagtggccta tgcaattctc atgtttgaca
     6241 gcttatcatc gatcgtccaa ctgcatggag atgagtcgtg gcaagaatac caagagttcc
     6301 tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata ctactcagtg
     6361 cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca ggtgggacag
     6421 gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag agccccgaga
     6481 gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat gcgcttagat
     6541 taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact
     6601 ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact gagtagtatt
     6661 tatttaagta ttgtttgtgc acttgcctgc aagccttttg aaaagcaagc ataaaagatc
     6721 taaacataaa atctgtaaaa taacaagatg taaagataat gctaaatcat ttggcttttt
     6781 gattgattgt acaggaaaat atacatcgca gggggttgac ttttaccatt tcaccgcaat
     6841 ggaatcaaac ttgttgaaga gaatgttcac aggcgcatac gctacaatga cccgattctt
     6901 gctagccttt tctcggtctt gcaaacaacc gccggcagct tagtatataa atacacatgt
     6961 acatacctct ctccgtatcc tcgtaatcat tttcttgtat ttatcgtctt ttcgctgtaa
     7021 aaactttatc acacttatct caaatacact tattaaccgc ttttactatt atcttctacg
     7081 ctgacagtaa tatcaaacag tgacacatat taaacacagt ggtttctttg cataaacacc
     7141 atcagcctca agtcgtcaag taaagatttc gtgttcatgc agatagataa caatctatat
     7201 gttgataatt agcgttgcct catcaatgcg agatccgttt aaccggaccc tagtgcactt
     7261 accccacgtt cggtccactg tgtgccgaac atgctccttc actattttaa catgtggaat
     7321 tatcgatgcc ggtgttattc ttgctatgat aggccatcga atgttaaagg gaagatttat
     7381 tgttttgccc tttaaagacg aatttggacc gtgaatgttt aaataattct catgtttgac
     7441 agcttatcat cgaactctaa gaggtgatac ttatttactg taaaactgtg acgataaaac
     7501 cggaaggaag aataagaaaa ctcgaactga tctataatgc ctattttctg taaagagttt
     7561 aagctatgaa agcctcggca ttttggccgc tcctaggtag tgcttttttt ccaaggacaa
     7621 aacagtttct ttttcttgag caggttttat gtttcggtaa tcataaacaa taaataaatt
     7681 atttcattta tgtttaaaaa taaaaaataa aaaagtattt taaattttta aaaaagttga
     7741 ttataagcat gtgacctttt gcaagcaatt aaattttgca atttgtgatt taggcaaaag
     7801 ttactatttc tggctcgtgt aatatatgta tgctaatgtg aacttttaca aagtcgatat
     7861 ggacttagtc aaaagaaatt ttcttaaaaa tatatagcac tagccaattt agcacttctt
     7921 tatgagatat attatagact ttattaagcc agatttgtgt attatatgta tttacccggc
     7981 gaatcatgga catacattct gaaataggta atattctcta tggtgagaca gcatagataa
     8041 cctaggatac aagttaaaag ctagtactgt tttgcagtaa tttttttctt ttttataaga
     8101 atgttaccac ctaaataagt tataaagtca atagttaagt ttgatatttg attgtaaaat
     8161 accgtaatat atttgcatga tcaaaaggct caatgttgac tagccagcat gtcaaccact
     8221 atattgatca ccgatattag gacttccaca ccaactagta atatgacaat aaattcaaga
     8281 tattcttcat gagaatggcc cagcgattat gcggtgtgaa ataccgcaca gatgcgtaag
     8341 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     8401 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     8461 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     8521 attcttatta cgatcctcgc gccccctacc caccgtactc gtcaattcca agggcatcgg
     8581 taaacatctg ctcaaactcg aagtcggcca tatccagagc gccgtagggg gcggagtcgt
     8641 ggggggtaaa tcccggaccc ggggaatccc cgtcccccaa catgtccaga tcgaaatcgt
     8701 ctagcgcgtc ggcatgcgcc atcgccacgt cctcgccgtc taagtggagc tcgtccccca
     8761 ggctgacatc ggtcgggggg gccgtcgaca gtctgcgcgt gtgtcccgcg gggagaaagg
     8821 acaggcgcgg agccgccagc cccgcctctt cgggggcgtc gtcgtccggg agatcgagca
     8881 ggccctcgat ggtagacccg taattgtttt tcgtacgcgc gcggctgtac gcggacccac
     8941 tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg
     9001 ctggctctgc accttggcga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
     9061 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttgt tcttccaata
     9121 cgcaacctaa agtaaagtgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa
     9181 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
     9241 gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac
     9301 ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag
     9361 tatggtgcct atctaacatc tcaatgggta aggcgtcgag caaagcccgc ttatttttta
     9421 catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta
     9481 aaccttcgat tccgacccca ttaagcagct ctaatgcgcc gttaatcact ttacttttat
     9541 ctaatctaga catatgaatt aattcggggc cgcggaggct ggatcggtcc cggtgtcttc
     9601 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
     9661 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     9721 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     9781 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     9841 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     9901 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     9961 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
    10021 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
    10081 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
    10141 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
    10201 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacatggc ggtaatgttg
    10261 gacatgagcc aatataaatg tacatattat gatatggata caacgtatgc aatgggccaa
    10321 gctcctcgag taattcgcgc cacttctaat aagcgatttc ttatgattta tgatttttat
    10381 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
    10441 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
    10501 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag
    10561 ggtaatatag atcaattcct cgatcgagtt taccactccc tatcagtgat agagaaaagt
    10621 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
    10681 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
    10741 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
    10801 cggtacccta tggcatgcat gtgctctgta tgtatataaa actcttgttt tcttcttt
//
