LOCUS       sequence-269629-        8961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BE4max-NRTH in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Miller SM, Wang T, Randolph PB, Arbab M, Shen MW, Huang TP, Matuszek
            Z, Newby GA, Rees HA, Liu DR
  TITLE     Continuous evolution of SpCas9 variants compatible with non-G PAMs.
  JOURNAL   Nat Biotechnol. 2020 Apr;38(4):471-481. doi: 
            10.1038/s41587-020-0412-8. Epub 2020 Feb 10.
  PUBMED    32042170
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0412-8"; journalName: "Nat Biotechnol"; date: 
            "2020-04"; volume: "38"; issue: "4"; pages: "471-481"
COMMENT     Sequence Label: pCMV-BE4max-NRTH
FEATURES             Location/Qualifiers
     source          1..8961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..714
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             715..1398
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             1495..5595
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLTIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMVKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQGDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRLRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKGNSDKLIARKKDWDPKKYGGFNSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIGFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASASVLHKGNEL
                     ALPSKYVNFLYLASHYEKLKGSSEDNKQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGASAAFKYFDTTIGRKLYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5626..5874
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5905..6153
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6196..6216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6225..6242
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6265..6282)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6271..6495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6566..6582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6566..6582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6579..6601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6590..6606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6614..6620,6621..6638,6639..6644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6659..6680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6797..6814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6968..7556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7048..7067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7727..8518,8519..8587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8350..8369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8588..8692)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8771..8790)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgttaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtctcctca
      721 gagactgggc ctgtcgccgt cgatccaacc ctgcgccgcc ggattgaacc tcacgagttt
      781 gaagtgttct ttgacccccg ggagctgaga aaggagacat gcctgctgta cgagatcaac
      841 tggggaggca ggcactccat ctggaggcac acctctcaga acacaaataa gcacgtggag
      901 gtgaacttca tcgagaagtt taccacagag cggtacttct gccccaatac cagatgtagc
      961 atcacatggt ttctgagctg gtccccttgc ggagagtgta gcagggccat caccgagttc
     1021 ctgtccagat atccacacgt gacactgttt atctacatcg ccaggctgta tcaccacgca
     1081 gacccaagga ataggcaggg cctgcgcgat ctgatcagct ccggcgtgac catccagatc
     1141 atgacagagc aggagtccgg ctactgctgg cggaacttcg tgaattattc tcctagcaac
     1201 gaggcccact ggcctaggta cccacacctg tgggtgcgcc tgtacgtgct ggagctgtat
     1261 tgcatcatcc tgggcctgcc cccttgtctg aatatcctgc ggagaaagca gccccagctg
     1321 accttcttta caatcgccct gcagtcttgt cactatcaga ggctgccacc ccacatcctg
     1381 tgggccacag gcctgaagtc tggaggatct agcggaggat cctctggcag cgagacacca
     1441 ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg cagcgacaag
     1501 aagtacagca tcggcctgac catcggcacc aactctgtgg gctgggccgt gatcaccgac
     1561 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
     1621 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
     1681 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
     1741 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
     1801 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     1861 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     1921 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     1981 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     2041 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     2101 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
     2161 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc
     2221 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     2281 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     2341 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     2401 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatggtgaag
     2461 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     2521 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt
     2581 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     2641 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     2701 accttcgaca acggcattat cccccaccag atccacctgg gagagctgca cgccattctg
     2761 cggcggcagg gcgattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     2821 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     2881 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     2941 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     3001 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag
     3061 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     3121 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     3181 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     3241 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     3301 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     3361 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     3421 gacgacaaag tgatgaagca gctgaagcgg ctgagataca ccggctgggg caggctgagc
     3481 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     3541 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     3601 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     3661 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     3721 gtggacgagc tcgtgaaagt gatgggcggc cacaagcccg agaacatcgt gatcgaaatg
     3781 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     3841 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     3901 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     3961 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct
     4021 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     4081 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     4141 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     4201 gaaagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     4261 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     4321 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     4381 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     4441 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     4501 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     4561 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     4621 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     4681 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     4741 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     4801 ggcttcagca aagagtctat cctgcccaag ggcaacagcg ataagctgat cgccagaaag
     4861 aaggactggg accctaagaa gtacggcggc ttcaacagcc ccaccgtggc ctattctgtg
     4921 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     4981 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcgg ctttctggaa
     5041 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     5101 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccagcgtgct gcataaggga
     5161 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     5221 aagctgaagg gctccagcga ggataataaa cagaaacagc tgtttgtgga acagcacaag
     5281 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     5341 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     5401 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc cagcgccgcc
     5461 ttcaagtact ttgacaccac catcggccgg aagctgtaca ccagcaccaa agaggtgctg
     5521 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     5581 cagctgggag gtgacagcgg cgggagcggc gggagcgggg ggagcactaa tctgagcgac
     5641 atcattgaga aggagactgg gaaacagctg gtcattcagg agtccatcct gatgctgcct
     5701 gaggaggtgg aggaagtgat cggcaacaag ccagagtctg acatcctggt gcacaccgcc
     5761 tacgacgagt ccacagatga gaatgtgatg ctgctgacct ctgacgcccc cgagtataag
     5821 ccttgggccc tggtcatcca ggattctaac ggcgagaata agatcaaaat gctgagcgga
     5881 ggatccggag gatctggagg cagcaccaac ctgtctgaca tcatcgagaa ggagacaggc
     5941 aagcagctgg tcatccagga gagcatcctg atgctgcccg aagaagtcga agaagtgatc
     6001 ggaaacaagc ctgagagcga tatcctggtc cataccgcct acgacgagag taccgacgaa
     6061 aatgtgatgc tgctgacatc cgacgcccca gagtataagc cctgggctct ggtcatccag
     6121 gattccaacg gagagaacaa aatcaaaatg ctgtctggcg gctcaaaaag aaccgccgac
     6181 ggcagcgaat tcgagcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc
     6241 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6301 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6361 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6421 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6481 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct
     6541 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat
     6661 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6721 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6781 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6961 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7561 atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca cgttaaggga
     7621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7801 ccgtcgtgta gataactaca atacgggagg gcttaccatc tggccccagt gctgcaatga
     7861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8341 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8701 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     8761 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     8821 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     8881 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     8941 tacgggccag atatacgcgt t
//
