LOCUS       sequence-269638-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ABEmax-NRCH in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Miller SM, Wang T, Randolph PB, Arbab M, Shen MW, Huang TP, Matuszek
            Z, Newby GA, Rees HA, Liu DR
  TITLE     Continuous evolution of SpCas9 variants compatible with non-G PAMs.
  JOURNAL   Nat Biotechnol. 2020 Apr;38(4):471-481. doi: 
            10.1038/s41587-020-0412-8. Epub 2020 Feb 10.
  PUBMED    32042170
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0412-8"; journalName: "Nat Biotechnol"; date: 
            "2020-04"; volume: "38"; issue: "4"; pages: "471-481"
COMMENT     Sequence Label: pCMV-ABEmax-NRCH
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1523..5623
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLTIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMVKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQGDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRLRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKGNSDKLIARKKDWDPKKYGGFNSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTINRKQYNTTKEVLD
                     ATLIRQSITGLYETRIDLSQLGGD"
     CDS             5666..5686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5695..5712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5735..5752)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5741..5965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6036..6052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6036..6052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6049..6071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6060..6076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6084..6090,6091..6108,6109..6114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6129..6150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6267..6284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6438..7026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6518..6537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7197..7988,7989..8057))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7820..7839
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8058..8162)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8241..8260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8432..8811
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtctctgaa gtcgagttta gccacgagta
      361 ttggatgagg cacgcactga ccctggcaaa gcgagcatgg gatgaaagag aagtccccgt
      421 gggcgccgtg ctggtgcaca acaatagagt gatcggagag ggatggaaca ggccaatcgg
      481 ccgccacgac cctaccgcac acgcagagat catggcactg aggcagggag gcctggtcat
      541 gcagaattac cgcctgatcg atgccaccct gtatgtgaca ctggagccat gcgtgatgtg
      601 cgcaggagca atgatccaca gcaggatcgg aagagtggtg ttcggagcac gggacgccaa
      661 gaccggcgca gcaggctccc tgatggatgt gctgcaccac cccggcatga accaccgggt
      721 ggagatcaca gagggaatcc tggcagacga gtgcgccgcc ctgctgagcg atttctttag
      781 aatgcggaga caggagatca aggcccagaa gaaggcacag agctccaccg actctggagg
      841 atctagcgga ggatcctctg gaagcgagac accaggcaca agcgagtccg ccacaccaga
      901 gagctccggc ggctcctccg gaggatcctc tgaggtggag ttttcccacg agtactggat
      961 gagacatgcc ctgaccctgg ccaagagggc acgcgatgag agggaggtgc ctgtgggagc
     1021 cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca
     1081 cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa
     1141 ctacagactg attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg
     1201 cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaacg caaaaaccgg
     1261 cgccgcaggc tccctgatgg acgtgctgca ctaccccggc atgaatcacc gcgtcgaaat
     1321 taccgaggga atcctggcag atgaatgtgc cgccctgctg tgctatttct ttcggatgcc
     1381 tagacaggtg ttcaatgctc agaagaaggc ccagagctcc accgactccg gaggatctag
     1441 cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag
     1501 cgggggcagc agcggggggt cagacaagaa gtacagcatc ggcctgacca tcggcaccaa
     1561 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     1621 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     1681 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     1741 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     1801 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     1861 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     1921 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     1981 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     2041 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     2101 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     2161 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     2221 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     2281 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     2341 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     2401 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     2461 ggcccccctg agcgcctcta tggtgaagag atacgacgag caccaccagg acctgaccct
     2521 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     2581 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     2641 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     2701 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcattatcc cccaccagat
     2761 ccacctggga gagctgcacg ccattctgcg gcggcagggc gatttttacc cattcctgaa
     2821 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     2881 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     2941 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     3001 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     3061 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     3121 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     3181 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     3241 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     3301 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     3361 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     3421 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggct
     3481 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     3541 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     3601 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     3661 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     3721 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggcggcca
     3781 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     3841 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     3901 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     3961 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     4021 cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     4081 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     4141 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     4201 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     4261 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     4321 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     4381 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     4441 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     4501 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     4561 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     4621 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     4681 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     4741 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     4801 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaaggg
     4861 taacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     4921 caacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     4981 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     5041 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     5101 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     5161 ggcctctgcc ggcgtgctgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     5221 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     5281 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     5341 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     5401 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     5461 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcaaccggaa
     5521 gcaatacaac acgaccaaag aggtgctgga cgccaccctg atccgtcaga gcatcaccgg
     5581 cctgtacgag acacggatcg acctgtctca gctgggaggt gactctggcg gctcaaaaag
     5641 aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg aaagtctaac cggtcatcat
     5701 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5761 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5821 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5881 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5941 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata
     6001 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6061 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag
     6121 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6181 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6241 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6301 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6361 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6421 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6481 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6601 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6721 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6781 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6841 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6901 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6961 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7021 tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa cgaaaactca
     7081 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7141 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7201 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7261 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7321 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7381 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7441 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7501 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7561 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7621 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7681 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7741 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7801 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7861 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7921 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7981 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8041 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8101 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8161 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat
     8221 cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8281 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8341 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8401 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     8461 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     8521 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     8581 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     8641 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     8701 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     8761 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat g
//
