LOCUS       sequence-269717-         399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GST tagged AO7 encoding aa 126-258 with an internal deletion that 
            results in loss of E2 binding for expression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Li S, Liang YH, Mariano J, Metzger MB, Stringer DK, Hristova VA, Li 
            J, Randazzo PA, Tsai YC, Ji X, Weissman AM
  TITLE     Insights into Ubiquitination from the Unique Clamp-like Binding of 
            the RING E3 AO7 to the E2 UbcH5B.
  JOURNAL   J Biol Chem. 2015 Dec 18;290(51):30225-39. doi: 
            10.1074/jbc.M115.685867. Epub 2015 Oct 16.
  PUBMED    26475854
REFERENCE   2  (bases 1 to 399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.685867"; journalName: "J Biol Chem"; date: 
            "2015-12-18- 18"; volume: "290"; issue: "51"; pages: "30225-39"
COMMENT     Sequence Label: pETDUET-GST AO7RE ∆238-245
FEATURES             Location/Qualifiers
     source          1..399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acagataaca acatccctca tggccagtgt gtcatctgcc tctatggttt ccaggagaag
       61 gaggccttta ccaaaacacc ctgttaccac tacttccact gccactgcct tgctcggtac
      121 atccagcaca tggagcaaga gctgaaggca caaggacagg agcaggaaca ggaacggcag
      181 catgctacaa ccaaacagaa ggcagtcggt gtgcagtgtc cagtgtgcag agagcccctc
      241 gtgtatgatc ttgcctcact gaaagcagcc cctgaacccc aacagcccat ggagctgtac
      301 cagcccagtg cagagagctt gcgccagcaa gaagaacgca agcggctcta ccagaggcag
      361 caggagcggg ggggaatcat tgaccttgag gctgagcga
//
