LOCUS       sequence-26974-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in
            transgenic mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Hohenstein P, Slight J, Ozdemir DD, Burn SF, Berry R, Hastie ND
  TITLE     High-efficiency Rosa26 knock-in vector construction for 
            Cre-regulated overexpression and RNAi.
  JOURNAL   Pathogenetics. 2008  . 1(1):3.
  PUBMED    19014667
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pathogenetics. 2008  . 1(1):3."
COMMENT     Sequence Label: pRosa26-DEST
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..550
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(86..109)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     483..502
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(627..646)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 atgtatgcta tacgaagtta ttaggtccct cgacctgcag gaattctacc gggtagggga
       61 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
      121 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
      181 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
      241 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
      301 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
      361 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
      421 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
      481 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
      541 gggcctttcg acctgcagcc aatatgggat cggccattga acaagatgga ttgcacgcag
      601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
      661 gctgctctga tgccgccgtt gttccggctg tcagcgcagg ggcgcccggt tctttttttg
      721 tcaagaccga cctgtccggt gccctgaatg aaactgcagg acgaggcagc gcggctatcg
      781 tggctgggca c
//
