LOCUS       sequence-269772-        3131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 RNAP-driven expression of SNIPR RNA with a LacZ reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Hong F, Ma D, Wu K, Mina LA, Luiten RC, Liu Y, Yan H, Green AA
  TITLE     Precise and Programmable Detection of Mutations Using Ultraspecific 
            Riboregulators.
  JOURNAL   Cell. 2020 Feb 25. pii: S0092-8674(20)30155-0. doi: 
            10.1016/j.cell.2020.02.011.
  PUBMED    32109416
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Feb 25. pii: S0092-8674(20)30155-0. doi: 
            10.1016/j.cell.2020.02.011."
COMMENT     Sequence Label: p_SNIPR_sensor_LacZ
FEATURES             Location/Qualifiers
     source          1..3131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..3084
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(30..47)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(30..46)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(39..61)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3085..3131
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgcgtaaaa tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
       61 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      121 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      181 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
      241 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
      301 gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg
      361 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
      421 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
      481 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
      541 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat
      601 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
      661 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
      721 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
      781 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
      841 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
      901 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
      961 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     1021 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     1081 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     1141 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     1201 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     1261 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     1321 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     1381 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     1441 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     1501 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     1561 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     1621 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     1681 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     1741 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     1801 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     1861 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     1921 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     1981 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     2041 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     2101 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     2161 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     2221 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     2281 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     2341 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     2401 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     2461 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     2521 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     2581 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     2641 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     2701 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     2761 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     2821 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     2881 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     2941 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     3001 gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac
     3061 cagttggtct ggtgtcaaaa ataatagcat aaccccttgg ggcctctaaa cgggtcttga
     3121 ggggtttttt g
//
