LOCUS       sequence-269788-        3408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus sequence of Hop (Humulus lupulus) tandem repeats for 
            cytogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Easterling KA, Pitra NJ, Morcol TB, Aquino JR, Lopes LG, Bussey KC, 
            Matthews PD, Bass HW
  TITLE     Identification of tandem repeat families from long-read sequences of
            Humulus lupulus.
  JOURNAL   PLoS One. 2020 Jun 5;15(6):e0233971. doi: 
            10.1371/journal.pone.0233971. eCollection 2020.
  PUBMED    32502183
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-06-5"; volume: "15(6):e0233971. doi"; pages: " 
            10.1371/journal.pone.0233971. eCollection 2020"
COMMENT     Sequence Label: pHTR120syn
FEATURES             Location/Qualifiers
     source          1..3408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(846..862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(846..862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(859..881)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    870..886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(894..900,901..918,919..924))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    939..960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1077..1094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1248..1836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1328..1347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2007..2798,2799..2867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2630..2649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2868..2972)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3040..3058
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3096..3118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3218..3237
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
       61 cttgcatgcc tgcaggtcga ctctagagga tccccaacca ggtcctaaac ttagaagttg
      121 attcaaattg attaatcgat gtttttctta aaagcacgag atatcaataa agaataacgc
      181 gaactaagtt ttccaaaatc aatgtcctgg atgcaaccag gtcctaaact tagaagttga
      241 ttcaaattga ttaatcgatg tttttcttaa aagcacgaga tatcaataaa gaataacgcg
      301 aactaagttt tccaaaatca atgtcctgga tgcttaacac gaactaagtt ttcaaaataa
      361 gttcctggtt ttaaccaggt cataaaactt agaagttaac ttaaatttat tgatcgtagt
      421 tcttccttta agagctcggg atatggataa aagttaacac gaactaagtt ttcaaaataa
      481 gttcctggtt ttaaccaggt cataaaactt agaagttaac ttaaatttat tgatcgtagt
      541 tcttccttta agagctcggg atatggataa aagtgttttt cttaaaaagc acgagatatc
      601 aataaagaat taacgtgaac taagtttttc aaaatcaata tcctggaaat taaccaggtc
      661 ctaaacttag aagttgattc aaattgatta atcgatgttt ttcttaaaaa gcacgagata
      721 tcaataaaga attaacgtga actaagtttt tcaaaatcaa tatcctggaa attaaccagg
      781 tcctaaactt agaagttgat tcaaattgat taatcgaggg taccgagctc gaattcgtaa
      841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      901 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      961 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2281 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2581 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2641 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3061 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3181 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3241 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3301 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3361 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgatta
//
