LOCUS       sequence-269814-        8884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human KIF1C 400-1103.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF1C tail 400-1103
FEATURES             Location/Qualifiers
     source          1..8884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1636..1658
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1726..1728,1729..1731,1732..2439)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1771..1792)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2032..2051)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2379..2400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3508..3517
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4720..4854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4757..4776)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4811..4830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4885..4901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4885..4901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4898..4920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4909..4925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4933..4939,4940..4957,4958..4963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4978..4999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5116..5133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5287..5875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5367..5386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6046..6837,6838..6906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6669..6688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6907..7011)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7079..7097
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7135..7157)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7257..7276
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7470..7492
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7484..7501
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7485..7501
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcactg cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
      721 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     1021 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     1081 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1141 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     1201 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1261 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1321 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1381 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1441 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1501 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1561 gtccccttct ccatctccag cctcggggct gtccgcaggg ggacggctgc cttcgggggg
     1621 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt
     1681 ctctgttcct ccgcagcccc caagcttggc gcgcctaggc ctaccatggt gagcaagggc
     1741 ggggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1801 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1861 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1921 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1981 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     2041 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2101 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2161 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2221 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2281 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2341 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2401 accgccgccg ggatcactct cggcatggac gagctgtacg gcggaggatc cggaggcggc
     2461 tctggcggag ggctgtacag tgccctgcca gctgtgtcat ctcccccagc tccagtttca
     2521 ccctcatcac ccaccacaca taatggggag ctggagccgt cattctcccc caacacggag
     2581 tcccagattg ggcctgagga agccatggag aggctgcagg agacagagaa gattatagct
     2641 gagctgaacg agacatggga ggagaagcta cgcaagacag aagccctgag gatggagaga
     2701 gaagcattgc tggctgagat gggggtggcc gtccgggagg atgggggaac tgtgggcgtc
     2761 ttctctccaa agaagactcc ccacctggtg aacctgaacg aagaccctct gatgtctgag
     2821 tgtctgctct accacatcaa agatggcgtc accagggtcg gccaagtaga tatggacatc
     2881 aagctgaccg gacagttcat tcgggagcaa cactgtctgt tccggagcat cccccagcca
     2941 gatggagaag tggtggtcac tctggagcct tgtgaaggag ctgagacata tgtgaatggg
     3001 aagcttgtga cggagccgct ggtgctgaag tcagggaata ggattgtgat gggcaagaac
     3061 cacgttttcc gcttcaacca cccggagcag gcaaggctgg aacgggaacg aggggtcccc
     3121 ccacccccag gaccgccctc tgagccagtc gactggaact ttgcccagaa ggaactgctg
     3181 gagcagcaag gcatcgacat aaagctggaa atggagaaga ggctgcagga tctggagaat
     3241 cagtaccgga aagaaaagga agaagccgat cttctgctgg agcagcagcg actgtatgca
     3301 gactcggaca gcggggatga ctctgacaag cgctcttgtg aagagagctg gaggctcatc
     3361 tcctccttgc gggagcagct gccgcccacc acggtccaga ccattgtcaa acgctgtggt
     3421 ctgcccagca gtggcaagcg cagggcccct cgcagggttt atcagatccc ccagcgacgc
     3481 aggctgcagg gcaaagaccc ccgctgggcc accatggctg acctgaagat gcaggcggtg
     3541 aaggagatct gctacgaggt ggccctggct gacttccgcc acgggcgggc tgagattgag
     3601 gccctggccg ccctcaagat gcgggagctg tgtcgcacct atggcaagcc agacggcccc
     3661 ggagacgcct ggagggctgt ggcccgggat gtctgggaca ctgtaggcga ggaggaagga
     3721 ggtggagctg gcagtggtgg tggcagtgag gagggagccc gaggggcgga ggtggaggac
     3781 ctccgggccc acatcgacaa gctgacgggg attctgcagg aggtgaagct gcagaacagc
     3841 agcaaggacc gggagctgca ggccctgcgg gaccgcatgc tccgcatgga gagggtcatc
     3901 cccctggccc aggatcatga ggatgagaat gaagaaggtg gtgaggtccc ctgggccccg
     3961 cctgaaggat cagaggcagc agaggaggca gcccccagtg accgcatgcc gtcagcccgg
     4021 cccccctcgc caccactgtc aagctgggag cgggtgtcac ggctcatgga ggaggaccct
     4081 gccttccgtc gtggtcgtct tcgctggctc aagcaggagc agctacggct gcagggactg
     4141 cagggctctg ggggccgggg cggggggctg cgcaggcccc cagcccgctt tgtgccccct
     4201 cacgactgca agctacgctt ccccttcaag agcaaccccc agcaccggga gtcttggcca
     4261 gggatgggga gcggggaggc tccaactccg ctccaacccc ctgaggaggt cactccccat
     4321 ccagccaccc ctgcccgccg gcctccgagt ccccgaaggt cccaccatcc ccgcaggaac
     4381 tccctggatg gagggggccg atcccgggga gcgggttctg cacagcctga accccagcac
     4441 ttccagccca aaaagcacaa ctcttatccc cagccacccc aaccctaccc agcccagcgg
     4501 cccccagggc cccgctaccc cccatacact actcccccac gaatgagacg gcagcgttct
     4561 gcccctgacc tcaaggagag tggggcagct gtgtgagtct ctagataagt aatgatcata
     4621 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4681 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4741 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4801 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccggg
     4861 taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     4921 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4981 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5041 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5461 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5701 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7021 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7081 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     7141 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     7261 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7321 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     7381 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7441 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     7501 tgccaagttg ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag
     7561 cacccggttc cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga
     7621 ggagccccct accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct
     7681 ctgatccaac cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc
     7741 atcctgccgg gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca
     7801 cggcttcatg gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc
     7861 tcccgctgta cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac
     7921 ccggccttgt caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac
     7981 agtgtgactc cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc
     8041 ccacggggtc ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct
     8101 ccgaggagac agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac
     8161 ctccagtttg gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg
     8221 gacaaagacc ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca
     8281 gtgcttcttg catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc
     8341 tggagctgca aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc
     8401 ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca
     8461 ttcggctgcc ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc
     8521 tcgcttcctc cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta
     8581 acgggctctg aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt
     8641 gcaccgctac gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc
     8701 ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat
     8761 cccagcaggc tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc
     8821 ctggtgcatg catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag
     8881 cagt
//
