LOCUS       sequence-269815-       12849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for constitutive FUCCI expression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Chang Y, Hellwarth PB, Randolph LN, Sun Y, Xing Y, Zhu W, Lian XL, 
            Bao X
  TITLE     Fluorescent indicators for continuous and lineage-specific reporting
            of cell-cycle phases in human pluripotent stem cells.
  JOURNAL   Biotechnol Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352.
  PUBMED    32277708
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352."
COMMENT     Sequence Label: AAVS1-Puro CAG FUCCI
FEATURES             Location/Qualifiers
     source          1..12849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..834
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    841..866
                     /label=SA
                     /note="splice acceptor site"
     CDS             890..943
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             953..1552
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(953..972)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1449..1469
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1584..1601)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1590..1814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1820..2199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2435..3451
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3375..3397
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3459..3478
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(3521..3523,3524..3526,3527..4237)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3566..3587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4174..4195
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4268..4597
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    4602..5174
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4769..4786)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4996..5015
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5189..5839
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             5189..5839
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             5894..6166
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     misc_feature    6204..6792
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6257..6277)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6675..6686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6824..7300
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(7023..7042)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(7410..7429)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7475..7530
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7529..7548)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7891..7907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7915..7931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7939..7945,7946..7963,7964..7969))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7984..8005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8079..8915
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8949..8968)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8950..8968)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8975..8992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8975..8991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8984..9006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9129..9431
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9310..9329
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(9834..9853)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10448..10467
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10828..11619,11620..11688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11451..11470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11812..12400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12301..12320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12554..12571
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12688..12709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12724..12729,12730..12747,12748..12754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12762..12778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12786..12802
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12823..12841
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agtcctgcag gtttaaacga attcgccctt tgctttctct gaccagcatt ctctcccctg
       61 ggcctgtgcc gctttctgtc tgcagcttgt ggcctgggtc acctctacgg ctggcccaga
      121 tccttccctg ccgcctcctt caggttccgt cttcctccac tccctcttcc ccttgctctc
      181 tgctgtgttg ctgcccaagg atgctctttc cggagcactt ccttctcggc gctgcaccac
      241 gtgatgtcct ctgagcggat cctccccgtg tctgggtcct ctccgggcat ctctcctccc
      301 tcacccaacc ccatgccgtc ttcactcgct gggttccctt ttccttctcc ttctggggcc
      361 tgtgccatct ctcgtttctt aggatggcct tctccgacgg atgtctccct tgcgtcccgc
      421 ctccccttct tgtaggcctg catcatcacc gtttttctgg acaaccccaa agtaccccgt
      481 ctccctggct ttagccacct ctccatcctc ttgctttctt tgcctggaca ccccgttctc
      541 ctgtggattc gggtcacctc tcactccttt catttgggca gctcccctac cccccttacc
      601 tctctagtct gtgctagctc ttccagcccc ctgtcatggc atcttccagg ggtccgagag
      661 ctcagctagt cttcttcctc caacccgggc ccctatgtcc acttcaggac agcatgtttg
      721 ctgcctccag ggatcctgtg tccccgagct gggaccacct tatattccca gggccggtta
      781 atgtggctct ggttctgggt acttttatct gtcccctcca ccccacagtg gggcaagctt
      841 ctgacctctt ctcttcctcc cacagggcct cgagagatct ggcagcggag agggcagagg
      901 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctaggctcg agatgaccga
      961 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     1021 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     1081 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     1141 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     1201 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     1261 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     1321 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     1381 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     1441 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     1501 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatctagagg
     1561 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1801 ggtgggctct atgggtctcg acattgatta ttgactagtt attaatagta atcaattacg
     1861 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1921 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1981 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2041 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2101 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2161 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2221 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2281 taattatttt gtgcagcgat gggggcgggg gggggggggg gcgaggcgga gaggtgcggc
     2341 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     2401 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
     2461 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
     2521 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
     2581 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
     2641 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     2701 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     2761 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     2821 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     2881 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
     2941 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
     3001 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
     3061 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     3121 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     3181 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     3241 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     3301 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     3361 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     3421 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     3481 gtctcatcat tttggcaaag aattcgtcga cgctagcacc atggtgagca agggcgagga
     3541 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3601 gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
     3661 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     3721 cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3781 cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta
     3841 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3901 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
     3961 cagccacaac gtctatatca cggccgacaa gcagaagaac ggcatcaagg ctaacttcaa
     4021 gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4081 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccgc
     4141 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4201 cgccgggatt acacatggca tggacgagct gtacaaggat atccatgcac actggcggcc
     4261 gctcgagatg aatcccagta tgaagcagaa acaagaagaa atcaaagaga atataaagaa
     4321 tagttctgtc ccaagaagaa ctctgaagat gattcagcct tctgcatctg gatctcttgt
     4381 tggaagagaa aatgagctgt ccgcaggctt gtccaaaagg aaacatcgga atgaccactt
     4441 aacatctaca acttccagcc ctggggttat tgtcccagaa tctagtgaaa ataaaaatct
     4501 tggaggagtc acccaggagt catttgatct tatgattaaa gaaaatccat cctctcagta
     4561 ttggaaggaa gtggcagaaa aacggagaaa ggcgctgtaa gcccctctcc ctcccccccc
     4621 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4681 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4741 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4801 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4861 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4921 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4981 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5041 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5101 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5161 aaacacgatg ataatggcca cagaattcat ggtgagcgtg atcaagcccg agatgaagat
     5221 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaatcgagg gtgagggcac
     5281 aggcagacct tacgagggac atcaggagat gacactgcgc gtcacaatgg ccgagggcgg
     5341 gccaatgcct ttcgccttcg acctggtgtc ccacgtgttc tgttacggcc acagagtttt
     5401 taccaagtac ccagaagaga tcccagacta tttcaagcag gcctttcctg agggcctgtc
     5461 ctgggagagg tccctggagt tcgaggacgg cggctccgcc tccgtgagcg cccacatcag
     5521 cctgaggggc aacaccttct accacaagtc caagttcacc ggcgtgaact tccccgccga
     5581 cggccccatc atgcagaacc agagcgtgga ctgggagccc tccaccgaga agatcaccgc
     5641 cagcgacggc gtgctgaagg gcgacgtgac catgtacctg aagctggagg gcggcggcaa
     5701 ccacaagtgc cagatgaaga ccacctacaa ggccgccaag gagatcctgg agatgcccgg
     5761 cgaccactac atcggccaca ggctggtgag gaagaccgag ggcaacatca ccgagcaggt
     5821 ggaggacgcc gtggcccact actcaatgtt gccttccctg ggcgatatcc atgcacactg
     5881 gcggccgctc gagcccagcc ccgccaggcc cgcactccgc gccccggcct ccgctaccag
     5941 tggcagccgc aagcgcgccc gcccgcccgc cgcccccgga cgcgaccagg ccaggccacc
     6001 ggcccgcagg agactgcggc tgtcggtgga cgaggtttcc agccccagta cccccgaggc
     6061 cccagacatc ccagcctgcc cttctccggg ccagaagata aagaaatcca ccccggcagc
     6121 aggtcagccg ccccacctga catccgcgca ggaccaggac accatctgag tttacgcgtg
     6181 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     6241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6601 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
     6661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6721 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6781 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
     6841 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     6901 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     6961 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     7021 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     7081 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     7141 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     7201 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     7261 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccag
     7321 gcgcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     7381 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     7441 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     7501 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     7561 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     7621 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     7681 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     7741 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     7801 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     7861 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8041 aaacctgtcg tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca
     8101 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     8161 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     8221 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     8281 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     8341 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     8401 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     8461 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     8521 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     8581 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     8641 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     8701 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     8761 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     8821 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     8881 tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat
     8941 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     9001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     9061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     9121 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     9181 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
     9241 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     9301 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     9361 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     9421 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
     9481 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     9541 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     9601 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     9661 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     9721 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
     9781 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     9841 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     9901 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     9961 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
    10021 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
    10081 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
    10141 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
    10201 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
    10261 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
    10321 gcgaggatct cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa
    10381 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
    10441 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
    10501 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    10561 cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt
    10621 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
    10681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc
    10741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11341 tgtcatgcca tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg
    11401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11701 tcaatattat tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga
    11761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    11881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    11941 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    12061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    12301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    12361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    12421 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    12481 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    12541 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    12601 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    12661 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    12721 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    12781 tcacacagga aacagctatg accatgatta cgccaagctc agaattaacc ctcactaaag
    12841 ggactagct
//
