LOCUS       sequence-269829-       12323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PhyB621-mCherry-HRasCT in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K
  TITLE     Improvement of Phycocyanobilin Synthesis for Genetically Encoded 
            Phytochrome-Based Optogenetics.
  JOURNAL   ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 
            10.1021/acschembio.0c00477. Epub 2020 Nov 9.
  PUBMED    33164485
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.0c00477"; journalName: "ACS Chem Biol"; date: 
            "2020-11-20- 20"; volume: "15"; issue: "11"; pages: "2896-2906"
COMMENT     Sequence Label: pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
FEATURES             Location/Qualifiers
     source          1..12323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2576..2890
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2584..2602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2620..2638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2691..2709
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2727..2745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2762..2780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2798..2816
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2863..2887
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4788..5492
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     primer_bind     complement(4933..4951)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5201..5220
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5636..6184
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5780..5797)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6007..6026
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6187..6981
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6241..6260)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6851..6870
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7017..7605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7070..7090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7488..7499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8124..8304
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8330..8347)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8336..8560
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8606..9034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8693..8712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8903..8924
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9043..9063)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9048..9378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9229..9364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9291..9310
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9426..9473
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9492..9866
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9996..10117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10033..10052)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10087..10106
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10166..10182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10166..10182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10179..10201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10190..10206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10214..10220,10221..10238,10239..10244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10259..10280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10397..10414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10568..11156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10648..10667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11327..12118,12119..12187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11950..11969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12188..12292)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagggaca gcagaaattc actttgaatt aattcgagtt
     2581 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2641 gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     2701 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     2761 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     2821 atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
     2881 tcagatcgcc tggagaattc accatggtgt caggagtggg aggaagcgga ggcggcagag
     2941 gaggcgggag aggaggcgag gaagagccaa gctcctctca cactcccaac aatcggagag
     3001 ggggagagca ggctcagagt tcaggcacta agtccctgag gccccgctct aacaccgaat
     3061 ccatgtctaa agccatccag cagtacaccg tggatgctag gctgcatgcc gtgtttgaac
     3121 agtccgggga gtctggaaag agtttcgact acagtcagtc actgaaaacc acaacttatg
     3181 ggagctccgt gcccgagcag cagattacag cttacctgag caggatccag aggggaggat
     3241 atattcagcc ttttggatgc atgatcgctg tggatgagtc tagtttccgg atcattgggt
     3301 actctgaaaa tgccagagag atgctgggaa tcatgccaca gagtgtgccc actctggaaa
     3361 agcctgagat tctggccatg ggcaccgacg tgcggtctct gtttacatca agctcctcta
     3421 tcctgctgga gcgggctttc gtggccagag aaatcactct gctgaaccca gtgtggattc
     3481 acagcaagaa taccggcaaa cccttttatg ccatcctgca taggattgac gtgggggtgg
     3541 tcatcgatct ggaacctgct cgcaccgagg atccagccct gagcattgct ggcgccgtgc
     3601 agtcccagaa gctggctgtg agagccatca gccagctgca ggccctgcca ggaggcgaca
     3661 tcaagctgct gtgcgataca gtggtggagt ccgtgaggga cctgactgga tacgatcgcg
     3721 tgatggtgta taagttccac gaagacgagc atggcgaagt ggtggccgag agcaaaaggg
     3781 acgatctgga gccttacatc ggcctgcact atcctgctac cgatattcca caggcctccc
     3841 gcttcctgtt taagcagaac cgggtgagaa tgatcgtgga ctgtaatgct acaccagtgc
     3901 tggtggtgca ggacgatcgg ctgacccaga gtatgtgcct ggtgggatca acactgagag
     3961 ccccccacgg ctgtcatagc cagtacatgg ctaacatggg gtcaattgcc agcctggcta
     4021 tggccgtgat cattaacggc aatgaggacg atgggagcaa tgtggcctcc gggaggagtt
     4081 caatgcgcct gtggggactg gtggtgtgcc accatacaag ctcccggtgt atcccatttc
     4141 ccctgagata tgcttgcgag tttctgatgc aggccttcgg cctgcagctg aacatggagc
     4201 tgcagctggc cctgcagatg agcgaaaagc gggtgctgag aacccagaca ctgctgtgcg
     4261 acatgctgct gagggattcc ccagctggaa tcgtgaccca gagcccttcc attatggacc
     4321 tggtgaagtg tgatggagcc gctttcctgt accacggcaa atactatcca ctgggagtgg
     4381 ctccttccga ggtgcagatc aaggatgtgg tggaatggct gctggctaat catgccgact
     4441 ctacaggcct gtccactgac tctctgggcg atgctggata ccctggagcc gctgccctgg
     4501 gagatgccgt gtgcggaatg gctgtggcct atattacaaa aagggacttc ctgttttggt
     4561 tccgcagcca cactgctaag gagatcaaat gggggggagc caagcaccat cctgaagata
     4621 aagacgatgg ccagcggatg catccaagat ctagttttca ggccttcctg gaggtggtga
     4681 agtctcggag tcagccatgg gaaaccgctg agatggatgc catccacagt ctgcagctga
     4741 ttctgagaga ctcttttaag gagagtggag gtagtggagg cggccgcatg gtgagcaagg
     4801 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     4861 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4921 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     4981 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     5041 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5101 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     5161 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     5221 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     5281 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     5341 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     5401 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     5461 gccactccac cggcggcatg gacgagctgt actctagaaa gctgaaccct cctgatgaga
     5521 gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg actagaggat ccgttaacta
     5581 acttaagcta gcaacggttt ccctctagcg ggatcaattc cgcccccccc ccctaacgtt
     5641 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     5701 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     5761 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     5821 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     5881 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     5941 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     6001 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     6061 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacgtgtgt ttagtcgagg
     6121 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6181 aataccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6241 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6301 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6361 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6421 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6481 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6541 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6601 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6661 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6721 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6781 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6841 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6901 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6961 cttcttgacg agttcttctg aactagagtt aacatcgagg gatcaagctt atcgataatc
     7021 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7081 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7141 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7201 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7261 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7321 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7381 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7441 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7501 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7561 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgag
     7621 acctggaaaa acatggagca atcacaagta gcaacacagc agctaccaat gctgcttgtg
     7681 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     7741 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7801 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     7861 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     7921 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     7981 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     8041 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     8101 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8161 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8221 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8281 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     8341 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8401 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     8461 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8521 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8581 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8761 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8821 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8881 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8941 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
     9001 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     9061 ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat
     9121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9181 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9301 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9361 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     9421 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     9481 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     9541 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     9601 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     9661 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     9721 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     9781 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     9841 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     9901 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9961 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    10021 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10081 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    10141 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    10201 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    10261 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    10321 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    10381 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10741 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
    10801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10981 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    11041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    11221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11641 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11941 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    12181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    12241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    12301 cccgaaaagt gccacctgac gtc
//
