LOCUS       sequence-269844-       22553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integration plasmid for expression of dLbCpf1-VP, 
            dSpCas9-RD1152, SaCas9, and Csy4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22553)
  AUTHORS   Lian J, Schultz C, Cao M, HamediRad M, Zhao H
  TITLE     Multi-functional genome-wide CRISPR system for high throughput 
            genotype-phenotype mapping.
  JOURNAL   Nat Commun. 2019 Dec 19;10(1):5794. doi: 10.1038/s41467-019-13621-4.
  PUBMED    31857575
REFERENCE   2  (bases 1 to 22553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-19"; volume: "10(1):5794. doi"; pages: " 
            10.1038/s41467-019-13621-4"
COMMENT     Sequence Label: pAID6
FEATURES             Location/Qualifiers
     source          1..22553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..48)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     148..167
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    201..234
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            289..1645
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        289..632
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             633..1442
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(700..719)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1448..1645
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1708..1741
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1836..1855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2068..2090
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2082..2099
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2083..2099
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2106..2125
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2106..2124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2174..2840
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2710..2733
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      2848..2857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2863..2883
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2902..6585
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             6586..6633
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6658..6678
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6712..6861
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6886..6906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7336..7692
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     terminator      7702..7867
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7757..7781)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      9075..9344
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     promoter        9482..9909
                     /gene="S. cerevisiae TPI1"
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose 
                     phosphate isomerase"
     CDS             9925..9945
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14062..14082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        15146..15544
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             15564..18725
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MGKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENN
                     EGRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEE
                     EFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDG
                     EVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPF
                     GWKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEK
                     FQIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARK
                     EIIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLK
                     AINLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQS
                     IKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKEN
                     AKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLV
                     KQEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINR
                     FSVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKE
                     RNKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKE
                     IFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDK
                     DNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSK
                     KDNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVK
                     NLDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDL
                     LNRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHP
                     QIIKKG"
     CDS             18732..18752
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             18759..18824
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(19352..19372)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19352..19370)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(19391..19407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19391..19407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(19404..19426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19415..19431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19439..19445,19446..19463,19464..19469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19484..19505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(19622..19639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19793..20381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19873..19892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(20552..21343,21344..21412))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21175..21194
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21413..21517)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    21727..22230
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     22523..22541
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
       61 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      121 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      181 ataccacagc ttttcaattc ataacttcgt ataatgtatg ctatacgaag ttattaggtc
      241 tagagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
      301 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
      361 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
      421 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
      481 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
      541 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
      601 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga
      661 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      721 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
      781 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      841 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      901 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag
      961 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1021 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     1081 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     1141 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     1201 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     1261 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1321 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1381 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1441 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     1501 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     1561 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     1621 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtgtc
     1681 gaaaacgagc tctcgagaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     1741 tggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1801 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1861 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1921 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     1981 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     2041 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2101 aattgtaata cgactcacta tagggcgaat tggagctcac tcggaattca gtctactagt
     2161 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
     2221 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
     2281 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
     2341 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
     2401 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
     2461 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
     2521 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
     2581 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
     2641 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
     2701 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
     2761 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
     2821 aacacacata aacaaacaaa acccgccgcc accatggctc ctccaaaaaa gaagagaaag
     2881 gtcggtagtg gttctggatc catgagcaag ctggagaagt ttacaaactg ctactccctg
     2941 tctaagaccc tgaggttcaa ggccatccct gtgggcaaga cccaggagaa catcgacaat
     3001 aagcggctgc tggtggagga cgagaagaga gccgaggatt ataagggcgt gaagaagctg
     3061 ctggatcgct actatctgtc ttttatcaac gacgtgctgc acagcatcaa gctgaagaat
     3121 ctgaacaatt acatcagcct gttccggaag aaaaccagaa ccgagaagga gaataaggag
     3181 ctggagaacc tggagatcaa tctgcggaag gagatcgcca aggccttcaa gggcaacgag
     3241 ggctacaagt ccctgtttaa gaaggatatc atcgagacaa tcctgccaga gttcctggac
     3301 gataaggacg agatcgccct ggtgaacagc ttcaatggct ttaccacagc cttcaccggc
     3361 ttctttgata acagagagaa tatgttttcc gaggaggcca agagcacatc catcgccttc
     3421 aggtgtatca acgagaatct gacccgctac atctctaata tggacatctt cgagaaggtg
     3481 gacgccatct ttgataagca cgaggtgcag gagatcaagg agaagatcct gaacagcgac
     3541 tatgatgtgg aggatttctt tgagggcgag ttctttaact ttgtgctgac acaggagggc
     3601 atcgacgtgt ataacgccat catcggcggc ttcgtgaccg agagcggcga gaagatcaag
     3661 ggcctgaacg agtacatcaa cctgtataat cagaaaacca agcagaagct gcctaagttt
     3721 aagccactgt ataagcaggt gctgagcgat cgggagtctc tgagcttcta cggcgagggc
     3781 tatacatccg atgaggaggt gctggaggtg tttagaaaca ccctgaacaa gaacagcgag
     3841 atcttcagct ccatcaagaa gctggagaag ctgttcaaga attttgacga gtactctagc
     3901 gccggcatct ttgtgaagaa cggccccgcc atcagcacaa tctccaagga tatcttcggc
     3961 gagtggaacg tgatccggga caagtggaat gccgagtatg acgatatcca cctgaagaag
     4021 aaggccgtgg tgaccgagaa gtacgaggac gatcggagaa agtccttcaa gaagatcggc
     4081 tccttttctc tggagcagct gcaggagtac gccgacgccg atctgtctgt ggtggagaag
     4141 ctgaaggaga tcatcatcca gaaggtggat gagatctaca aggtgtatgg ctcctctgag
     4201 aagctgttcg acgccgattt tgtgctggag aagagcctga agaagaacga cgccgtggtg
     4261 gccatcatga aggacctgct ggattctgtg aagagcttcg agaattacat caaggccttc
     4321 tttggcgagg gcaaggagac aaacagggac gagtccttct atggcgattt tgtgctggcc
     4381 tacgacatcc tgctgaaggt ggaccacatc tacgatgcca tccgcaatta tgtgacccag
     4441 aagccctact ctaaggataa gttcaagctg tattttcaga accctcagtt catgggcggc
     4501 tgggacaagg ataaggagac agactatcgg gccaccatcc tgagatacgg ctccaagtac
     4561 tatctggcca tcatggataa gaagtacgcc aagtgcctgc agaagatcga caaggacgat
     4621 gtgaacggca attacgagaa gatcaactat aagctgctgc ccggccctaa taagatgctg
     4681 ccaaaggtgt tcttttctaa gaagtggatg gcctactata accccagcga ggacatccag
     4741 aagatctaca agaatggcac attcaagaag ggcgatatgt ttaacctgaa tgactgtcac
     4801 aagctgatcg acttctttaa ggatagcatc tcccggtatc caaagtggtc caatgcctac
     4861 gatttcaact tttctgagac agagaagtat aaggacatcg ccggctttta cagagaggtg
     4921 gaggagcagg gctataaggt gagcttcgag tctgccagca agaaggaggt ggataagctg
     4981 gtggaggagg gcaagctgta tatgttccag atctataaca aggacttttc cgataagtct
     5041 cacggcacac ccaatctgca caccatgtac ttcaagctgc tgtttgacga gaacaatcac
     5101 ggacagatca ggctgagcgg aggagcagag ctgttcatga ggcgcgcctc cctgaagaag
     5161 gaggagctgg tggtgcaccc agccaactcc cctatcgcca acaagaatcc agataatccc
     5221 aagaaaacca caaccctgtc ctacgacgtg tataaggata agaggttttc tgaggaccag
     5281 tacgagctgc acatcccaat cgccatcaat aagtgcccca agaacatctt caagatcaat
     5341 acagaggtgc gcgtgctgct gaagcacgac gataacccct atgtgatcgg catcgctagg
     5401 ggcgagcgca atctgctgta tatcgtggtg gtggacggca agggcaacat cgtggagcag
     5461 tattccctga acgagatcat caacaacttc aacggcatca ggatcaagac agattaccac
     5521 tctctgctgg acaagaagga gaaggagagg ttcgaggccc gccagaactg gacctccatc
     5581 gagaatatca aggagctgaa ggccggctat atctctcagg tggtgcacaa gatctgcgag
     5641 ctggtggaga agtacgatgc cgtgatcgcc ctggaggacc tgaactctgg ctttaagaat
     5701 agccgcgtga aggtggagaa gcaggtgtat cagaagttcg agaagatgct gatcgataag
     5761 ctgaactaca tggtggacaa gaagtctaat ccttgtgcaa caggcggcgc cctgaagggc
     5821 tatcagatca ccaataagtt cgagagcttt aagtccatgt ctacccagaa cggcttcatc
     5881 ttttacatcc ctgcctggct gacatccaag atcgatccat ctaccggctt tgtgaacctg
     5941 ctgaaaacca agtataccag catcgccgat tccaagaagt tcatcagctc ctttgacagg
     6001 atcatgtacg tgcccgagga ggatctgttc gagtttgccc tggactataa gaacttctct
     6061 cgcacagacg ccgattacat caagaagtgg aagctgtact cctacggcaa ccggatcaga
     6121 atcttccgga atcctaagaa gaacaacgtg ttcgactggg aggaggtgtg cctgaccagc
     6181 gcctataagg agctgttcaa caagtacggc atcaattatc agcagggcga tatcagagcc
     6241 ctgctgtgcg agcagtccga caaggccttc tactctagct ttatggccct gatgagcctg
     6301 atgctgcaga tgcggaacag catcacaggc cgcaccgacg tggattttct gatcagccct
     6361 gtgaagaact ccgacggcat cttctacgat agccggaact atgaggccca ggagaatgcc
     6421 atcctgccaa agaacgccga cgccaatggc gcctataaca tcgccagaaa ggtgctgtgg
     6481 gccatcggcc agttcaagaa ggccgaggac gagaagctgg ataaggtgaa gatcgccatc
     6541 tctaacaagg agtggctgga gtacgcccag accagcgtga agcacaaaag gccggcggcc
     6601 acgaaaaagg ccggccaggc aaaaaagaaa aagaagcttg gcggcagcgg cggcagcccg
     6661 aaaaagaaac gcaaagttgg gcgcgccgag gccagcggtt ccggacgggc tgacgcattg
     6721 gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg
     6781 cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat
     6841 gatttcgacc tggacatgct gattaactct agaagttccg gatctccgaa aaagaaacgc
     6901 aaagttggta gccagtacct gcccgacacc gacgaccggc accggatcga ggaaaagcgg
     6961 aagcggacct acgagacatt caagagcatc atgaagaagt cccccttcag cggccccacc
     7021 gaccctagac ctccacctag aagaatcgcc gtgcccagca gatccagcgc cagcgtgcca
     7081 aaacctgccc cccagcctta ccccttcacc agcagcctga gcaccatcaa ctacgacgag
     7141 ttccctacca tggtgttccc cagcggccag atctctcagg cctctgctct ggctccagcc
     7201 cctcctcagg tgctgcctca ggctcctgct cctgcaccag ctccagccat ggtgtctgca
     7261 ctggctcagg caccagcacc cgtgcctgtg ctggctcctg gacctccaca ggctgtggct
     7321 ccaccagccc ctaaacctac acaggccggc gagggcacac tgtctgaagc tctgctgcag
     7381 ctgcagttcg acgacgagga tctgggagcc ctgctgggaa acagcaccga tcctgccgtg
     7441 ttcaccgacc tggccagcgt ggacaacagc gagttccagc agctgctgaa ccagggcatc
     7501 cctgtggccc ctcacaccac cgagcccatg ctgatggaat accccgaggc catcacccgg
     7561 ctcgtgacag gcgctcagag gcctcctgat ccagctcctg cccctctggg agcaccaggc
     7621 ctgcctaatg gactgctgtc tggcgacgag gacttcagct ctatcgccga tatggatttc
     7681 tcagccttgc tgtgactcga ggcgaatttc ttatgattta tgatttttat tattaaataa
     7741 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     7801 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag
     7861 gtcgctctta ttgaccacac ctctaccctg cagggtgtcg acgctgcggg tatagaaagg
     7921 gttctttact ctatagtacc tcctcgctca gcatctgctt cttcccaaag atgaacgcgg
     7981 cgttatgtca ctaacgacgt gcaccatttt tgcggaaagt ggaatcccgt tccaaaactg
     8041 gcatccacta attgatacat ctacacaccg cacgcctttt ttctgaagcc cactttcgtg
     8101 gactttgcca tatgcaaaat tcatgaagtg tgataccaag tcagcataca cctcactagg
     8161 gtagtttctt tggttgtatt gatcatttgg ttcatcgtgg ttcattaatt ttttttctcc
     8221 attgctttct ggctttgatc ttactatcat ttggattttt gtcgaaggtt gtagaattgt
     8281 atgtgacaag tggcaccaag catatataaa aaaaaaaagc attatcttcc taccagagtt
     8341 gattgttaaa aacgtattta tagcaaacgc aattgtaatt aattcttatt ttgtatcttt
     8401 tcttcccttg tctcaatctt ttatttttat tttatttttc ttttcttagt ttctttcata
     8461 acaccaagca actaatacta taacatacaa taataggatc tatgggtgat cattatctgg
     8521 atattcggct gaggcctgat ccagagttcc cacctgcgca gctgatgtct gtcctttttg
     8581 gcaaacttca tcaggccctg gttgcccagg gcggagatcg gataggggta agctttccag
     8641 acctcgacga aagccggagc cgcctgggag aacgcctgcg gatccacgct tctgccgacg
     8701 atctgagagc cttgctggca aggccatggc ttgaggggct ccgggatcac ctgcagtttg
     8761 gcgaacccgc cgttgttccc cacccaaccc cttatcggca ggtgtctaga gtgcaggcca
     8821 aatctaatcc agaacggctg cgacggcgac tcatgcggcg acatgatctt agcgaggaag
     8881 aggcccgaaa aagaatccct gataccgtgg cccgcgccct tgacttgcct tttgtcacac
     8941 tgcggtccca gagtacgggg cagcatttca gacttttcat tcgacacggg ccactgcaag
     9001 ttaccgccga agaaggaggc tttacttgtt atggactctc caagggaggt ttcgtgccct
     9061 ggttttaact cgagattgaa ttgaattgaa atcgatagat caattttttt cttttctctt
     9121 tccccatcct ttacgctaaa ataatagttt attttatttt tgaatatttt ttatttatat
     9181 acgtatatat agactattat ttatctttta atgattatta agatttttat taaaaaaaaa
     9241 ttcgctcctc ttttaatgcc tttatgcagt ttttttttcc cattcgatat ttctatgttc
     9301 gggttcagcg tattttaagt ttaataactc gaaaattctg cgttcgttaa agctttcgag
     9361 aaggatatta tttcgaaata aaccgtgttg tgtaagcttg aagccttttt gcgctgccaa
     9421 tattcttatc catctattgt actctttaga tccagtatag tgtattcttc ctggcggccg
     9481 ctatatctag gaacccatca ggttggtgga agattacccg ttctaagact tttcagcttc
     9541 ctctattgat gttacacctg gacacccttt tctggcatcc agtttttaat cttcagtggc
     9601 atgtgagatt ctccgaaatt aattaaagca atcacacaat tctctcggat accacctcgg
     9661 ttgaaactga caggtggttt gttacgcatg ctaatgcaaa ggagcctata tacctttggc
     9721 tcggctgctg taacagggaa tataaagggc agcataattt aggagtttag tgaacttgca
     9781 acatttacta ttttcccttc ttacgtaaat atttttcttt ttaattctaa atcaatcttt
     9841 ttcaattttt tgtttgtatt cttttcttgc ttaaatctat aactacaaaa aacacataca
     9901 taactaaaag gatccatgga cgtcccaaag aagaagagaa aggtaggcag cgacaagaag
     9961 tattctatcg gactggccat cgggactaat agcgtcgggt gggccgtgat cactgacgag
    10021 tacaaggtgc cctctaagaa gttcaaggtg ctcgggaaca ccgaccggca ttccatcaag
    10081 aaaaatctga tcggagctct cctctttgat tcaggggaga ccgctgaagc aacccgcctc
    10141 aagcggactg ctagacggcg gtacaccagg aggaagaacc ggatttgtta ccttcaagag
    10201 atattctcca acgaaatggc aaaggtcgac gacagcttct tccataggct ggaagaatca
    10261 ttcctcgtgg aagaggataa gaagcatgaa cggcatccca tcttcggtaa tatcgtcgac
    10321 gaggtggcct atcacgagaa atacccaacc atctaccatc ttcgcaaaaa gctggtggac
    10381 tcaaccgaca aggcagacct ccggcttatc tacctggccc tggcccacat gatcaagttc
    10441 agaggccact tcctgatcga gggcgacctc aatcctgaca atagcgatgt ggataaactg
    10501 ttcatccagc tggtgcagac ttacaaccag ctctttgaag agaaccccat caatgcaagc
    10561 ggagtcgatg ccaaggccat tctgtcagcc cggctgtcaa agagccgcag acttgagaat
    10621 cttatcgctc agctgccggg tgaaaagaaa aatggactgt tcgggaacct gattgctctt
    10681 tcacttgggc tgactcccaa tttcaagtct aatttcgacc tggcagagga tgccaagctg
    10741 caactgtcca aggacaccta tgatgacgat ctcgacaacc tcctggccca gatcggtgac
    10801 caatacgccg accttttcct tgctgctaag aatctttctg acgccatcct gctgtctgac
    10861 attctccgcg tgaacactga aatcaccaag gcccctcttt cagcttcaat gattaagcgg
    10921 tatgatgagc accaccagga cctgaccctg cttaaggcac tcgtccggca gcagcttccg
    10981 gagaagtaca aggaaatctt ctttgaccag tcaaagaatg gatacgccgg ctacatcgac
    11041 ggaggtgcct cccaagagga attttataag tttatcaaac ctatccttga gaagatggac
    11101 ggcaccgaag agctcctcgt gaaactgaat cgggaggatc tgctgcggaa gcagcgcact
    11161 ttcgacaatg ggagcattcc ccaccagatc catcttgggg agcttcacgc catccttcgg
    11221 cgccaagagg acttctaccc ctttcttaag gacaacaggg agaagattga gaaaattctc
    11281 actttccgca tcccctacta cgtgggaccc ctcgccagag gaaatagccg gtttgcttgg
    11341 atgaccagaa agtcagaaga aactatcact ccctggaact tcgaagaggt ggtggacaag
    11401 ggagccagcg ctcagtcatt catcgaacgg atgactaact tcgataagaa cctccccaat
    11461 gagaaggtcc tgccgaaaca ttccctgctc tacgagtact ttaccgtgta caacgagctg
    11521 accaaggtga aatatgtcac cgaagggatg aggaagcccg cattcctgtc aggcgaacaa
    11581 aagaaggcaa ttgtggacct tctgttcaag accaatagaa aggtgaccgt gaagcagctg
    11641 aaggaggact atttcaagaa aattgaatgc ttcgactctg tggagattag cggggtcgaa
    11701 gatcggttca acgcaagcct gggtacctac catgatctgc ttaagatcat caaggacaag
    11761 gattttctgg acaatgagga gaaagaggac atccttgagg acattgtcct gactctcact
    11821 ctgttcgagg accgggaaat gatcgaggag aggcttaaga cctacgccca tctgttcgac
    11881 gataaagtga tgaagcaact taaacggaga agatataccg gatggggacg ccttagccgc
    11941 aaactcatca acggaatccg ggacaaacag agcggaaaga ccattcttga tttccttaag
    12001 agcgacggat tcgctaatcg caacttcatg caacttatcc atgatgattc cctgaccttt
    12061 aaggaggaca tccagaaggc ccaagtgtct ggacaaggtg actcactgca cgagcatatc
    12121 gcaaatctgg ctggttcacc cgctattaag aagggtattc tccagaccgt gaaagtcgtg
    12181 gacgagctgg tcaaggtgat gggtcgccat aaaccagaga acattgtcat cgagatggcc
    12241 agggaaaacc agactaccca gaagggacag aagaacagca gggagcggat gaaaagaatt
    12301 gaggaaggga ttaaggagct cgggtcacag atccttaaag agcacccggt ggaaaacacc
    12361 cagcttcaga atgagaagct ctatctgtac taccttcaaa atggacgcga tatgtatgtg
    12421 gaccaagagc ttgatatcaa caggctctca gactacgacg tggacgccat cgtccctcag
    12481 agcttcctca aagacgactc aattgacaat aaggtgctga ctcgctcaga caagaaccgg
    12541 ggaaagtcag ataacgtgcc ctcagaggaa gtcgtgaaaa agatgaagaa ctattggcgc
    12601 cagcttctga acgcaaagct gatcactcag cggaagttcg acaatctcac taaggctgag
    12661 aggggcggac tgagcgaact ggacaaagca ggattcatta aacggcaact tgtggagact
    12721 cggcagatta ctaaacatgt agcccaaatc cttgactcac gcatgaatac caagtacgac
    12781 gaaaacgaca aacttatccg cgaggtgaag gtgattaccc tgaagtccaa gctggtcagc
    12841 gatttcagaa aggactttca attctacaaa gtgcgggaga tcaataacta tcatcatgct
    12901 catgacgcat atctgaatgc cgtggtggga accgccctga tcaagaagta cccaaagctg
    12961 gaaagcgagt tcgtgtacgg agactacaag gtctacgacg tgcgcaagat gattgccaaa
    13021 tctgagcagg agatcggaaa ggccaccgca aagtacttct tctacagcaa catcatgaat
    13081 ttcttcaaga ccgaaatcac ccttgcaaac ggtgagatcc ggaagaggcc gctcatcgag
    13141 actaatgggg agactggcga aatcgtgtgg gacaagggca gagatttcgc taccgtgcgc
    13201 aaagtgcttt ctatgcctca agtgaacatc gtgaagaaaa ccgaggtgca aaccggaggc
    13261 ttttctaagg aatcaatcct ccccaagcgc aactccgaca agctcattgc aaggaagaag
    13321 gattgggacc ctaagaagta cggcggattc gattcaccaa ctgtggctta ttctgtcctg
    13381 gtcgtggcta aggtggaaaa aggaaagtct aagaagctca agagcgtgaa ggaactgctg
    13441 ggtatcacca ttatggagcg cagctccttc gagaagaacc caattgactt tctcgaagcc
    13501 aaaggttaca aggaagtcaa gaaggacctt atcatcaagc tcccaaagta tagcctgttc
    13561 gaactggaga atgggcggaa gcggatgctc gcctccgctg gcgaacttca gaagggtaat
    13621 gagctggctc tcccctccaa gtacgtgaat ttcctctacc ttgcaagcca ttacgagaag
    13681 ctgaagggga gccccgagga caacgagcaa aagcaactgt ttgtggagca gcataagcat
    13741 tatctggacg agatcattga gcagatttcc gagttttcta aacgcgtcat tctcgctgat
    13801 gccaacctcg ataaagtcct tagcgcatac aataagcaca gagacaaacc aattcgggag
    13861 caggctgaga atatcatcca cctgttcacc ctcaccaatc ttggtgcccc tgccgcattc
    13921 aagtacttcg acaccaccat cgaccggaaa cgctatacct ccaccaaaga agtgctggac
    13981 gccaccctca tccaccagag catcaccgga ctttacgaaa ctcggattga cctctcacag
    14041 ctcggagggg atgagggagc tcccaagaaa aagcgcaagg taggtagttc caagcttagt
    14101 ggtggaggaa gtggcgggtc agggtcgaat tctgcatctt catctaccaa actagacgac
    14161 gacttgggta cagcagcagc agtgctatca aacatgagat catccccata tagaactcat
    14221 gataaaccca tttccaatgt caatgacatg aataacacaa atgcgctcgg tgtgccggct
    14281 agtaggcctc attcgtcatc ttttccatca aagggtgtct taagaccaat tctgttacgt
    14341 atccataatt ccgaacaaca acccattttc gaaagcaaca attctacatc gggaggtggt
    14401 tcgggtggct ctggatcaga ttcacaagtt caagaactgg aaacattacc acccataaga
    14461 agtttaccgt tgcccttccc acacatggac tcaggcggtg gtagtggtgg gagcggtagt
    14521 aagcttggcg gcagcggcgg cagctacgaa gaagagatca agcacttgaa actagggctg
    14581 gagcaaagag accatcaaat tgcatctttg accgtccagc aacagcagca acagcaacag
    14641 cagcaacagc aacagcagca acaggtccag cagcatttac aacagcaaca acagcagcta
    14701 gccgctgcat ctgcatctgt tccagttgcg taactcgagg attaatataa ttatataaaa
    14761 atattatctt cttttcttta tatctagtgt tatgtaaaat aaattgatga ctacggaaag
    14821 cttttttata ttgtttcttt ttcattctga gccacttaaa tttcgtgaat gttcttgtaa
    14881 gggacggtag atttacaagt gatacaacaa aaagcaaggc gctttttcta ataaaaagaa
    14941 gaaaagcatt taacaattga acacctctat atcaacgaag aatattactt tgtctctaaa
    15001 tccttgtaaa atgtgtacga tctctatatg ggttactcat aagtgtaccg aagactgcat
    15061 tgaaagttta tgttttttca ctggaggcgt cattttcgcg ttgagaagat gttcttatcc
    15121 aaatttcaac tgttatatac cgcggatagc ttcaaaatgt ttctactcct tttttactct
    15181 tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat
    15241 actaaattcc ccctctttct tcctctaggg tgtcgttaat tacccgtact aaaggtttgg
    15301 aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaagggcaa taaaaatttt
    15361 tatcacgttt ctttttcttg aaaatttttt tttttgattt ttttctcttt cgatgacctc
    15421 ccattgatat ttaagttatt aaatggtctt caatttctca agtttcagtt tcatttttct
    15481 tgttctatta caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc
    15541 taagttttaa ttacaaagga tccatgggga aacggaacta catcctgggg cttgacattg
    15601 ggataaccag cgttggctac ggaattattg attatgagac acgcgatgtg attgacgccg
    15661 gggttaggct gttcaaagag gccaacgttg aaaacaacga gggaagacgg agtaagcgcg
    15721 gagcaagaag actcaagcgc agacggagac atcggattca gagggtgaaa aagctgctct
    15781 tcgattacaa tctcctgacc gatcatagtg agctgagcgg aatcaacccc tacgaggcgc
    15841 gagtgaaagg gctttcccag aagctgtccg aagaggagtt ctccgccgcg ttgctgcacc
    15901 tggccaaacg gaggggggtt cacaatgtaa acgaagtgga ggaggacacg ggcaatgaac
    15961 ttagtacgaa agaacagatc agtaggaact ctaaggctct cgaagagaaa tacgtcgctg
    16021 agttgcagct tgagagactg aaaaaagacg gcgaagtacg cggatctatt aataggttca
    16081 agacttcaga ttacgtaaag gaagccaagc agctcctgaa agtacagaaa gcgtaccatc
    16141 agctcgatca gagcttcatc gatacctaca tagatttgct ggagacacgg aggacatact
    16201 acgagggccc aggggaagga tctccttttg ggtggaagga catcaaggaa tggtacgaga
    16261 tgcttatggg acattgtaca tattttccgg aggagctcag gagcgtcaag tacgcctaca
    16321 atgccgacct gtacaatgcc ctcaatgacc tcaataacct cgtgattacc agggacgaga
    16381 acgagaagct ggagtactat gaaaagttcc agattatcga gaatgtgttt aagcagaaga
    16441 agaagccgac acttaagcag attgcaaagg aaatcctcgt gaatgaggaa gatatcaagg
    16501 gatacagagt gacaagtaca ggcaagcccg agttcacaaa tctgaaggtg taccacgata
    16561 ttaaggacat aaccgcacga aaggagataa tcgaaaacgc tgagctcctc gatcagatcg
    16621 caaaaattct taccatctac cagtctagtg aggacattca ggaggaactg actaatctga
    16681 acagtgagct cacccaagag gaaattgagc agatttcaaa cctgaaaggc tacaccggga
    16741 cgcacaatct gagcctcaaa gcaatcaacc tcattctgga tgaactttgg cacacaaatg
    16801 acaaccaaat tgccatattc aaccgcctga aactggtgcc aaaaaaagtg gatctgtcac
    16861 agcaaaagga aatccctaca accttggttg acgattttat tctgtccccc gttgtcaagc
    16921 ggagcttcat ccagtcaatc aaggtgatca atgccatcat taaaaaatac ggattgccaa
    16981 acgatataat tatcgagctt gcacgagaga agaactcaaa ggacgcccag aagatgatta
    17041 acgaaatgca gaagcgcaac cgccagacaa acgaacgcat agaggaaatt ataagaacaa
    17101 ccggcaaaga gaatgccaag tatctgatcg agaaaatcaa gctgcacgac atgcaagaag
    17161 gcaagtgcct gtactctctg gaagctatcc cactcgaaga tctgctgaat aatccattca
    17221 attacgaggt ggaccacatc atccctagat ccgtaagctt tgacaattcc ttcaataaca
    17281 aagttctggt taaacaggag gaaaattcta aaaaagggaa ccggaccccg ttccagtacc
    17341 tgagctccag tgacagcaag attagctacg agacttttaa gaaacatatt ctgaatctgg
    17401 ccaaaggcaa aggcaggatc agcaagacca agaaggagta cctcctcgaa gaacgcgaca
    17461 ttaacagatt tagtgtgcag aaagatttca tcaaccgaaa ccttgtcgat actcggtacg
    17521 ccacgagagg cctgatgaat ctcctcagga gctacttccg cgtcaataat ctggacgtta
    17581 aagtcaagag cataaatggg ggattcacca gctttctgag gagaaagtgg aagtttaaga
    17641 aggaacgaaa caaaggatac aagcaccatg ctgaggatgc tttgatcatc gctaacgcgg
    17701 actttatctt taaggaatgg aaaaagctgg ataaggcaaa gaaagtgatg gaaaaccaga
    17761 tgttcgagga gaagcaggca gagtcaatgc ctgagatcga gacagagcag gaatacaagg
    17821 aaattttcat cacccctcat cagattaaac acataaagga cttcaaagac tataaatact
    17881 ctcatagggt ggacaaaaaa cccaatcgcg agctcattaa tgacaccctg tactcaacac
    17941 ggaaggatga taaaggtaat accttgattg tgaataatct taatggattg tatgacaaag
    18001 ataacgacaa gctcaagaag ctgatcaaca agtctccaga gaagctcctt atgtatcacc
    18061 acgacccaca gacttatcag aaattgaaac tgatcatgga gcaatacggg gatgagaaga
    18121 acccactcta caaatattat gaggaaacag gtaattacct gaccaagtac tccaagaagg
    18181 ataacggacc agtgatcaaa aagataaagt actatggcaa caaacttaat gcgcatttgg
    18241 acataactga cgattacccc aattctcgaa acaaggttgt gaagctctcc ctgaagcctt
    18301 atagatttga cgtgtacctg gataatgggg tttataaatt cgtcaccgtg aaaaatctgg
    18361 acgtgatcaa aaaggagaac tattatgaag taaactcaaa gtgctatgag gaggcgaaga
    18421 agctgaagaa gatctccaat caggccgagt tcatcgcttc cttctataat aacgatctca
    18481 tcaagatcaa tggagagctt tatcgcgtca ttggtgtgaa caatgacttg ctgaacagga
    18541 tcgaagtcaa tatgatagac attacctacc gggagtatct cgaaaacatg aatgataaac
    18601 ggccgcctcg catcatcaag acaatcgcat ctaaaactca gtcaataaaa aagtactcta
    18661 ccgatatcct ggggaatctc tatgaagtga agtcaaagaa gcacccacaa atcattaaaa
    18721 aaggtggatc ccccaagaag aagaggaaag tctcgagcga ctacaaagac catgacggtg
    18781 attataaaga tcatgacatc gattacaagg atgacgatga caagtaagtc gagggagatt
    18841 gataagactt ttctagttgc atatctttta tatttaaatc ttatctatta gttaattttt
    18901 tgtaatttat ccttatatat agtctggtta ttctaaaata tcatttcagt atctaaaaat
    18961 tcccctcttt tttcagttat atcttaacag gcgacagtcc aaatgttgat ttatcccagt
    19021 ccgattcatc agggttgtga agcattttgt caatggtcga aatcacatca gtaatagtgc
    19081 ctcttacttg cctcatagaa tttctttctc ttaacgtcac cgtttggtct tttatagttt
    19141 cgaaatctat ggtgatacca aatggtgttc ccaattcatc gttacgggcg tattttttac
    19201 caattgaagt attggaatcg tcaattttaa agtatatctc tcttttacgt aaagcctgcg
    19261 agatcctctt aagtatagcg gggaagccat cgttattcga tattgtcgta acaaatactt
    19321 tgatcggcgc tatcccgggc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
    19381 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    19441 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    19501 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    19561 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    19621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    19681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    19741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    19801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    19861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    19921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    19981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    20041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    20101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    20161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    20221 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    20281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    20341 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    20401 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    20461 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    20521 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    20581 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    20641 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    20701 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    20761 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    20821 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    20881 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    20941 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    21001 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    21061 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    21121 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    21181 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    21241 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    21301 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    21361 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    21421 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    21481 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    21541 ctgtggaagc tgaaacgtct aacggatctt gatttgtgtg gacttcctta gaagtaaccg
    21601 aagcacaggc gctaccatga gaattgggtg aatgttgaga taattgttgg gattccattg
    21661 ttgataaagg ctataatatt aggtatacag aatatactag aagttctcgt ttaaacggtc
    21721 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
    21781 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
    21841 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
    21901 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
    21961 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
    22021 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
    22081 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
    22141 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
    22201 gaggcgcgtg taagttacag gcaagcgatc cgtccgttta aacctcgagg atataggaat
    22261 cctcaaaatg gaatctgcaa ttctacacaa ttctataaat attattatca tcattttata
    22321 tgtttatatt cattgatcct attacattat caatccttgc gtttcagctt ccactaattt
    22381 agatgactat ttctcatcat ttgcgtcatc ttctaacacc gtatatgata atatactagt
    22441 aatgtaaata ctagttagta gatgatagtt gatttctatt ccaacataag aaaccattat
    22501 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcg
//
