LOCUS       sequence-269849-       11767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (D61P)
FEATURES             Location/Qualifiers
     source          1..11767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        703..1082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1083..1286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1237..1257
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1283..1307
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1389..1418
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(2466..2468,2469..2927)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(2508..2529)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2769..2788)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2950..3084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2974..2993)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3028..3047
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3227..3249)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3809..5598
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5900..7825)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGAGXGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGXGGXGGAGGAGXGGAGAGG
                     GAGGXGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGXGGAGXGGXGGAGAGGGAGGAGXG
                     GGAGGAGAGGXGGXGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8260..8278)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8346..8450
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8451..8519,8520..9311)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8669..8688)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9482..10070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9971..9990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10501..10646
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10689..11726
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11724..11743)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
       61 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
      121 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
      181 gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg
      241 ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg
      301 gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg
      361 accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc
      421 cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg
      481 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
      541 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      601 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      661 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      721 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      781 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      841 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      901 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      961 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1021 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1081 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
     1141 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1201 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     1261 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg
     1321 taccagctgc tagcgccacc atgaagacca tcatcgccct gagctacatc ttctgcctgg
     1381 tgttcgccga gcagaaattg atcagcgagg aggacctggg cagccctgga ggcagttcag
     1441 gaggcggatc tcactccatg aggtatttct tcacatccgt gtcccggccc ggccgcgggg
     1501 agccccgctt catcgcagtg ggctacgtgg acgacacgca gttcgtgcgg ttcgacagcg
     1561 acgccgcgag ccagaggatg gagccgcggg cgccgtggat agagcaggag ggtccggagt
     1621 attggcccgg ggagacacgg aaagtgaagg cccactcaca gactcaccga gtggacctgg
     1681 ggaccctgcg cggctactac aaccagagcg aggccggttc tcacaccgtc cagaggatgt
     1741 atggctgcga cgtggggtcg gactggcgct tcctccgcgg gtaccaccag tacgcctacg
     1801 acggcaagga ttacatcgcc ctgaaagagg acctgcgctc ttggaccgcg gcggacatgg
     1861 cagctcagac caccaagcac aagtgggagg cggcccatgt ggcggagcag ttgagagcct
     1921 acctggaggg cacgtgcgtg gagtggctcc gcagatacct ggagaacggg aaggagacgc
     1981 tgcagcgtac ggacgccccc aaaacgcata tgactcacca cgctgtctct gaccatgaag
     2041 ccaccctgag gtgctgggcc ctgagcttct accctgcgga gatcacactg acctggcagc
     2101 gggatgggga ggaccagacc caggacacgg agctcgtgga gaccaggcct gcaggggatg
     2161 gaaccttcca gaagtgggcg gctgtggtgg tgccttctgg acaggagcag agatacacct
     2221 gccatgtgca gcatgagggt ttgcccaagc ccctcaccct gagatgggag ccgtcttccc
     2281 agcccaccat ccccatcgtg ggcatcattg ctggcctggt tctctttgga gctgtgatca
     2341 ctggagctgt ggtcgctgct gtgatgtgga ggaggaagag ctcagataga aaaggaggga
     2401 gctactctca ggctgcaagc agtgacagtg cccagggctc tgatgtgtct ctcacagctt
     2461 gtaaagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2521 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2581 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
     2641 ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     2701 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2761 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2821 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2881 acaagctgga gtacaactac aacagccaca acgtctatct gaccgcctaa ctcgaggccg
     2941 gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3001 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3061 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     3181 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3241 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3301 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg
     3361 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     3421 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     3481 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     3541 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     3601 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     3661 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     3721 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     3781 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     3841 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     3901 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     3961 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     4021 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     4081 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     4141 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     4201 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     4261 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     4321 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     4381 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     4441 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     4501 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     4561 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     4621 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     4681 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     4741 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     4801 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     4861 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     4921 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4981 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5041 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5101 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     5161 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     5221 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5281 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5341 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     5401 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     5461 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
     5521 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
     5581 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
     5641 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
     5701 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
     5761 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
     5821 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
     5881 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
     5941 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
     6001 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
     6061 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
     6121 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
     6181 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
     6241 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
     6301 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
     6361 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
     6421 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
     6481 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
     6541 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
     6601 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
     6661 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
     6721 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
     6781 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
     6841 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
     6901 ctgcccctcc tgctcctgcc cctcctgccc ctcctsctcc tscccctcct scccctcctg
     6961 ctcctgcccc tcctgcccct cctcctsctc ctgcccctcc tgcccctcct cctgctcctg
     7021 cccctcctsc ccctcctsct cctgcccctc ctscccctcc tgctcctgcc cctcctgccc
     7081 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     7141 ctcctscccc tcctgcccct cctcctgctc ctgcccctcc tsctcctgcc cctcctgccc
     7201 ctcctscccc tcctsctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
     7261 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctsctcctg
     7321 cccctcctsc ccctcctscc cctcctccts ctcctscccc tcctscccct cctcctsctc
     7381 ctscycctcc tcctsctcct scycctccts cycctcctsc ycctcctcct sctcctsccc
     7441 ctcctcctsc tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     7501 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg
     7561 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
     7621 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
     7681 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
     7741 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
     7801 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
     7861 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
     7921 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
     7981 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
     8041 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
     8101 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
     8161 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
     8221 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     8281 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8341 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8401 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8461 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8521 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8581 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8641 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     8701 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8761 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8821 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8881 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8941 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     9001 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9061 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9121 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9181 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9241 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9301 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9361 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9421 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9481 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9541 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9601 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     9661 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9721 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9781 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9841 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9901 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9961 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10021 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10081 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
    10141 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
    10201 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
    10261 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
    10321 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
    10381 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
    10441 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
    10501 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
    10561 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
    10621 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
    10681 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
    10741 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
    10801 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
    10861 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    10921 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    10981 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
    11041 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    11101 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    11161 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    11221 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    11281 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    11341 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    11401 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    11461 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    11521 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    11581 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    11641 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
    11701 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    11761 gctatga
//
