LOCUS       sequence-269851-       11767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (E166P)
FEATURES             Location/Qualifiers
     source          1..11767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1764..1766,1767..2225)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(1806..1827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2067..2086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2248..2382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2272..2291)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2326..2345
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2525..2547)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3107..4896
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5198..7123)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGXGGGXGXGGGXGGXGGXGXGGGXGXGGGXGGXGXGGGX
                     GGXGGAGXGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGXGGXGGAGGAGXGGAGAGG
                     GAGGXGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGXGGAGXGGXGGAGAGGGAGGAGXG
                     GGAGGAGAGGXGGXGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7558..7576)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7644..7748
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7749..7817,7818..8609)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7967..7986)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8780..9368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9269..9288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9799..9944
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9987..11024
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11022..11041)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11066..11114
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cagttcagga ggcggatctc actccatgag gtatttcttc acatccgtgt
      781 cccggcccgg ccgcggggag ccccgcttca tcgcagtggg ctacgtggac gacacgcagt
      841 tcgtgcggtt cgacagcgac gccgcgagcc agaggatgga gccgcgggcg ccgtggatag
      901 agcaggaggg tccggagtat tgggacgggg agacacggaa agtgaaggcc cactcacaga
      961 ctcaccgagt ggacctgggg accctgcgcg gctactacaa ccagagcgag gccggttctc
     1021 acaccgtcca gaggatgtat ggctgcgacg tggggtcgga ctggcgcttc ctccgcgggt
     1081 accaccagta cgcctacgac ggcaaggatt acatcgccct gaaagaggac ctgcgctctt
     1141 ggaccgcggc ggacatggca gctcagacca ccaagcacaa gtgggaggcg gcccatgtgg
     1201 cggagcagtt gagagcctac ctggagggca cgtgcgtgcc ttggctccgc agatacctgg
     1261 agaacgggaa ggagacgctg cagcgtacgg acgcccccaa aacgcatatg actcaccacg
     1321 ctgtctctga ccatgaagcc accctgaggt gctgggccct gagcttctac cctgcggaga
     1381 tcacactgac ctggcagcgg gatggggagg accagaccca ggacacggag ctcgtggaga
     1441 ccaggcctgc aggggatgga accttccaga agtgggcggc tgtggtggtg ccttctggac
     1501 aggagcagag atacacctgc catgtgcagc atgagggttt gcccaagccc ctcaccctga
     1561 gatgggagcc gtcttcccag cccaccatcc ccatcgtggg catcattgct ggcctggttc
     1621 tctttggagc tgtgatcact ggagctgtgg tcgctgctgt gatgtggagg aggaagagct
     1681 cagatagaaa aggagggagc tactctcagg ctgcaagcag tgacagtgcc cagggctctg
     1741 atgtgtctct cacagcttgt aaagtgagca agggcgagga gctgttcacc ggggtggtgc
     1801 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1861 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     1921 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
     1981 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2041 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2101 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2161 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatctga
     2221 ccgcctaact cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     2281 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2341 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2401 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     2461 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     2521 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2581 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     2641 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     2701 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     2761 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     2821 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     2881 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     2941 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3001 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3061 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3121 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3181 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3241 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3301 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3361 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3421 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3481 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3541 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3601 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3661 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     3721 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     3781 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     3841 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     3901 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     3961 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4021 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4081 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4141 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4201 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4261 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4321 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4381 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4441 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4501 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4621 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4681 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4741 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
     4801 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
     4861 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
     4921 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     4981 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     5041 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     5101 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     5161 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     5221 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     5281 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     5341 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     5401 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     5461 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
     5521 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
     5581 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
     5641 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
     5701 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
     5761 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     5821 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     5881 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     5941 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     6001 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     6061 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     6121 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc
     6181 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctsctccts
     6241 cccctcctsc ccctcctgct cctgcccctc ctgcccctcc tcctsctcct gcccctcctg
     6301 cccctcctcc tgctcctgcc cctcctsccc ctcctsctcc tgcccctcct scccctcctg
     6361 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
     6421 cccctcctgc tcctgcccct cctscccctc ctgcccctcc tcctgctcct gcccctccts
     6481 ctcctgcccc tcctgcccct cctscccctc ctsctcctgc ccctcctcct gctcctgccc
     6541 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     6601 cccctcctcc tsctcctgcc cctcctsccc ctcctscccc tcctcctsct cctscccctc
     6661 ctscccctcc tcctsctcct scycctcctc ctsctcctsc ycctcctscy cctcctscyc
     6721 ctcctcctsc tcctscccct cctcctsctc ctgcccctcc tgcccctcct gcccctcctg
     6781 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg
     6841 ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt
     6901 tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc
     6961 ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc
     7021 ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc
     7081 ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag
     7141 atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc
     7201 cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct
     7261 gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa
     7321 aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc
     7381 tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct
     7441 tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt
     7501 gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc
     7561 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7621 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7681 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7741 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7801 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7861 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7921 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7981 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8041 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8101 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8161 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8221 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8281 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8341 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8401 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8461 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8521 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8581 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8641 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8701 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8761 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8821 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8881 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8941 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9001 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa
     9421 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
     9481 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
     9541 acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga
     9601 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     9661 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc
     9721 ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg
     9781 catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg
     9841 aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg
     9901 cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg
     9961 ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg
    10021 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
    10081 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
    10141 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
    10201 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
    10261 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
    10321 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
    10381 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
    10441 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
    10501 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
    10561 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
    10621 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
    10681 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
    10741 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
    10801 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
    10861 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
    10921 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
    10981 gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca
    11041 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
    11101 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
    11161 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
    11221 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
    11281 cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg
    11341 gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca
    11401 tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt
    11461 ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa
    11521 gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg
    11581 ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg
    11641 caattagcca tattagtcat tggttatata gcataaatca atattggcta ttggccattg
    11701 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
    11761 ccatgtt
//
