LOCUS       sequence-269858-        9434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and
            used with the Flp-In T-Rex System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pcDNA5 FRT-TO-EGFP-UPF1-WT
FEATURES             Location/Qualifiers
     source          1..9434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1016..1148
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1131..1150
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1193..1211
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1227..1236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1233..1235,1236..1238,1239..1949)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1233..1235,1236..1238,1239..1949)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1278..1299)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1539..1558)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1886..1907
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5386..5403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5392..5616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5749..5768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5900..5947
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5955..6977
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5955..6977
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7107..7228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7144..7163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7198..7217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7277..7293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7277..7293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7290..7312)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7301..7317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7325..7331,7332..7349,7350..7355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7370..7391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7508..7525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7679..8267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7759..7778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8438..9229,9230..9298))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9061..9080
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9299..9403)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttcttgcag aagttggtcg tgaggcactg ggcaggtaag
     1021 tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag
     1081 aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct
     1141 ctccacaggt gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga
     1201 ctcactatag gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
     1261 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1321 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1381 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1441 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1501 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1561 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1621 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1681 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1741 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1801 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1861 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1921 tcactctcgg catggacgag ctgtacaagc ttagcgtgga ggcgtacggg cccagctcgc
     1981 agactctcac tttcctggac acggaggagg ccgagctgct tggcgccgac acacagggct
     2041 ccgagttcga gttcaccgac tttactcttc ctagccagac gcagacgccc cccggcggcc
     2101 ccggcggccc gggcggtggc ggcgcgggag gcccgggcgg cgcgggcgcg ggcgctgcgg
     2161 cgggacagct cgacgcgcag gttgggcccg aaggcatcct gcagaacggg gctgtggacg
     2221 acagtgtagc caagaccagc cagttgttgg ctgagttgaa cttcgaggaa gatgaagaag
     2281 acacctatta cacgaaggac ctccccatac acgcctgcag ttactgtgga atacacgatc
     2341 ctgcctgcgt ggtttactgt aataccagca agaagtggtt ctgcaacgga cgtggaaata
     2401 cttctggcag ccacattgta aatcaccttg tgagggcaaa atgcaaagag gtgaccctgc
     2461 acaaggacgg gcccctgggg gagacagtcc tggagtgcta caactgcggc tgtcgcaacg
     2521 tcttcctcct cggcttcatc ccggccaaag ctgactcagt ggtggtgctg ctgtgcaggc
     2581 agccctgtgc cagccagagc agcctcaagg acatcaactg ggacagctcg cagtggcagc
     2641 cgctgatcca ggaccgctgc ttcctgtcct ggctggtcaa gatcccctcc gagcaggagc
     2701 agctgcgggc acgccagatc acggcacagc agatcaacaa gctggaggag ctgtggaagg
     2761 aaaacccttc tgccacgctg gaggacctgg agaagccggg ggtggacgag gagccgcagc
     2821 atgtcctcct gcggtacgag gacgcctacc agtaccagaa catattcggg cccctggtca
     2881 agctggaggc cgactacgac aagaagctga aggagtccca gactcaagat aacatcactg
     2941 tcaggtggga cctgggcctt aacaagaaga gaatcgccta cttcactttg cccaagactg
     3001 actctgacat gcggctcatg cagggggatg agatatgcct gcggtacaaa ggggaccttg
     3061 cgcccctgtg gaaagggatc ggccacgtca tcaaggtccc tgataattat ggcgatgaga
     3121 tcgccattga gctgcggagc agcgtgggtg cacctgtgga ggtgactcac aacttccagg
     3181 tggattttgt gtggaagtcg acctcctttg acaggatgca gagcgcattg aaaacgtttg
     3241 ccgtggatga gacctcggtg tctggctaca tctaccacaa gctgttgggc cacgaggtgg
     3301 aggacgtaat catcaagtgc cagctgccca agcgcttcac ggcgcagggc ctccccgacc
     3361 tcaaccactc ccaggtttat gccgtgaaga ctgtgctgca aagaccactg agcctgatcc
     3421 agggcccgcc aggcacgggg aagacggtga cgtcggccac catcgtctac cacctggccc
     3481 ggcaaggcaa cgggccggtg ctggtgtgtg ctccgagcaa catcgccgtg gaccagctaa
     3541 cggagaagat ccaccagacg gggctaaagg tcgtgcgcct ctgcgccaag agccgtgagg
     3601 ccatcgactc cccggtgtct tttctggccc tgcacaacca gatcaggaac atggacagca
     3661 tgcctgagct gcagaagctg cagcagctga aagacgagac tggggagctg tcgtctgccg
     3721 acgagaagcg gtaccgggcc ttgaagcgca ccgcagagag agagctgctg atgaacgcag
     3781 atgtcatctg ctgcacatgt gtgggcgccg gtgacccgag gctggccaag atgcagttcc
     3841 gctccatttt aatcgacgaa agcacccagg ccaccgagcc ggagtgcatg gttcccgtgg
     3901 tcctcggggc caagcagctg atccttgtag gcgaccactg ccagctgggc ccagtggtga
     3961 tgtgcaagaa ggcggccaag gccgggctgt cacagtcgct cttcgagcgc ctggtggtgc
     4021 tgggcatccg gcccatccgc ctgcaggtcc agtaccggat gcaccctgca ctcagcgcct
     4081 tcccatccaa catcttctac gagggctccc tccagaatgg tgtcactgca gcggatcgtg
     4141 tgaagaaggg atttgacttc cagtggcccc aacccgataa accgatgttc ttctacgtga
     4201 cccagggcca agaggagatt gccagctcgg gcacctccta cctgaacagg accgaggctg
     4261 cgaacgtgga gaagatcacc acgaagttgc tgaaggcagg cgccaagccg gaccagattg
     4321 gcatcatcac gccctacgag ggccagcgct cctacctggt gcagtacatg cagttcagcg
     4381 gctccctgca caccaagctc taccaggagg tggagatcgc cagtgtggac gcctttcagg
     4441 gacgcgagaa ggacttcatc atcctgtcct gtgtgcgggc caacgagcac caaggcattg
     4501 gctttttaaa tgaccccagg cgtctgaacg tggccctgac cagagcaagg tatggcgtca
     4561 tcattgtggg caacccgaag gcactatcaa agcagccgct ctggaaccac ctgctgaact
     4621 actataagga gcagaaggtg ctggtggagg ggccgctcaa caacctgcgt gagagcctca
     4681 tgcagttcag caagccacgg aagctggtca acactatcaa cccgggagcc cgcttcatga
     4741 ccacagccat gtatgatgcc cgggaggcca tcatcccagg ctccgtctat gatcggagca
     4801 gccagggccg gccttccagc atgtacttcc agacccatga ccagattggc atgatcagtg
     4861 ccggccctag ccacgtggct gccatgaaca ttcccatccc cttcaacctg gtcatgccac
     4921 ccatgccacc gcctggctat tttggacaag ccaacgggcc tgctgcaggg cgaggcaccc
     4981 cgaaaggcaa gactggtcgt gggggacgcc agaagaaccg ctttgggctt cctggaccca
     5041 gccagactaa cctccccaac agccaagcca gccaggatgt ggcgtcacag cccttctctc
     5101 agggcgccct gacgcagggc tacatctcca tgagccagcc ttcccagatg agccagcccg
     5161 gcctctccca gccggagctg tcccaggaca gttaccttgg tgacgagttt aaatcacaaa
     5221 tcgacgtggc gctctcacag gactccacgt accagggaga gcgggcttac cagcatggcg
     5281 gggtgacggg gctgtcccag tattaaaagg tggcggcgga agagctaaag agtcgacccg
     5341 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     5401 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5461 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5521 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5581 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5641 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5701 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5761 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5821 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5881 gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta gaaagtatag
     5941 gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     6001 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     6061 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     6121 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     6181 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     6241 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     6301 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     6361 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     6421 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     6481 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     6541 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     6601 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     6661 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     6721 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     6781 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     6841 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     6901 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     6961 cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc ttctatgaaa
     7021 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7081 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7201 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7261 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7321 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7381 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7441 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7501 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7561 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7621 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7681 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7741 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7801 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7861 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7921 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7981 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8041 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8101 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8161 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8341 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8401 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8461 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8521 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8581 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8641 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8701 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8761 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8821 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8881 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8941 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9001 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9061 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9121 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9181 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9241 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9301 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9361 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9421 tgccacctga cgtc
//
