LOCUS       sequence-269863-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus 
            and used with the Flp-In T-Rex System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pcDNA5 FRT-TO-EGFP-UPF1-R615A
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        124..503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     658..678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    709..727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    730..748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     758..782
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          905..1037
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1020..1039
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1082..1100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1116..1125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1122..1124,1125..1127,1128..1838)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1122..1124,1125..1127,1128..1838)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1167..1188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1428..1447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1775..1796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5287..5304)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5293..5517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5650..5669)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5801..5848
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5856..6878
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5856..6878
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7008..7129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7045..7064)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7099..7118
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7178..7194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7178..7194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7191..7213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7202..7218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7226..7232,7233..7250,7251..7256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7271..7292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7409..7426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7580..8168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7660..7679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8339..9130,9131..9199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8962..8981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9200..9304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9379..9398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
       61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      121 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      181 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      241 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      301 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      361 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      421 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      661 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      721 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      781 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      841 gcctccggac tctagcgttt aaacttaagc ttcttgcaga agttggtcgt gaggcactgg
      901 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
      961 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     1021 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
     1081 ttaatacgac tcactatagg ctagcgctac cggtcgccac catggtgagc aagggcgagg
     1141 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1201 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1261 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1321 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1381 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1441 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1501 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1561 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1621 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1681 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1741 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1801 ccgccgggat cactctcggc atggacgagc tgtacaagct tagcgtgaag cttagcgtgg
     1861 aggcgtacgg gcccagctcg cagactctca ctttcctgga cacggaggag gccgagctgc
     1921 ttggcgccga cacacagggc tccgagttcg agttcaccga ctttactctt cctagccaga
     1981 cgcagacgcc ccccggcggc cccggcggcc cgggcggtgg cggcgcggga ggcccgggcg
     2041 gcgcgggcgc gggcgctgcg gcgggacagc tcgacgcgca ggttgggccc gaaggcatcc
     2101 tgcagaacgg ggctgtggac gacagtgtag ccaagaccag ccagttgttg gctgagttga
     2161 acttcgagga agatgaagaa gacacctatt acacgaagga cctccccata cacgcctgca
     2221 gttactgtgg aatacacgat cctgcctgcg tggtttactg taataccagc aagaagtggt
     2281 tctgcaacgg acgtggaaat acttctggca gccacattgt aaatcacctt gtgagggcaa
     2341 aatgcaaaga ggtgaccctg cacaaggacg ggcccctggg ggagacagtc ctggagtgct
     2401 acaactgcgg ctgtcgcaac gtcttcctcc tcggcttcat cccggccaaa gctgactcag
     2461 tggtggtgct gctgtgcagg cagccctgtg ccagccagag cagcctcaag gacatcaact
     2521 gggacagctc gcagtggcag ccgctgatcc aggaccgctg cttcctgtcc tggctggtca
     2581 agatcccctc cgagcaggag cagctgcggg cacgccagat cacggcacag cagatcaaca
     2641 agctggagga gctgtggaag gaaaaccctt ctgccacgct ggaggacctg gagaagccgg
     2701 gggtggacga ggagccgcag catgtcctcc tgcggtacga ggacgcctac cagtaccaga
     2761 acatattcgg gcccctggtc aagctggagg ccgactacga caagaagctg aaggagtccc
     2821 agactcaaga taacatcact gtcaggtggg acctgggcct taacaagaag agaatcgcct
     2881 acttcacttt gcccaagact gactctgaca tgcggctcat gcagggggat gagatatgcc
     2941 tgcggtacaa aggggacctt gcgcccctgt ggaaagggat cggccacgtc atcaaggtcc
     3001 ctgataatta tggcgatgag atcgccattg agctgcggag cagcgtgggt gcacctgtgg
     3061 aggtgactca caacttccag gtggattttg tgtggaagtc gacctccttt gacaggatgc
     3121 agagcgcatt gaaaacgttt gccgtggatg agacctcggt gtctggctac atctaccaca
     3181 agctgttggg ccacgaggtg gaggacgtaa tcatcaagtg ccagctgccc aagcgcttca
     3241 cggcgcaggg cctccccgac ctcaaccact cccaggttta tgccgtgaag actgtgctgc
     3301 aaagaccact gagcctgatc cagggcccgc caggcacggg gaagacggtg acgtcggcca
     3361 ccatcgtcta ccacctggcc cggcaaggca acgggccggt gctggtgtgt gctccgagca
     3421 acatcgccgt ggaccagcta acggagaaga tccaccagac ggggctaaag gtcgtgcgcc
     3481 tctgcgccaa gagccgtgag gccatcgact ccccggtgtc ttttctggcc ctgcacaacc
     3541 agatcaggaa catggacagc atgcctgagc tgcagaagct gcagcagctg aaagacgaga
     3601 ctggggagct gtcgtctgcc gacgagaagc ggtaccgggc cttgaagcgc accgcagagg
     3661 cagagctgct gatgaacgca gatgtcatct gctgcacatg tgtgggcgcc ggtgacccga
     3721 ggctggccaa gatgcagttc cgctccattt taatcgacga aagcacccag gccaccgagc
     3781 cggagtgcat ggttcccgtg gtcctcgggg ccaagcagct gatccttgta ggcgaccact
     3841 gccagctggg cccagtggtg atgtgcaaga aggcggccaa ggccgggctg tcacagtcgc
     3901 tcttcgagcg cctggtggtg ctgggcatcc ggcccatccg cctgcaggtc cagtaccgga
     3961 tgcaccctgc actcagcgcc ttcccatcca acatcttcta cgagggctcc ctccagaatg
     4021 gtgtcactgc agcggatcgt gtgaagaagg gatttgactt ccagtggccc caacccgata
     4081 aaccgatgtt cttctacgtg acccagggcc aagaggagat tgccagctcg ggcacctcct
     4141 acctgaacag gaccgaggct gcgaacgtgg agaagatcac cacgaagttg ctgaaggcag
     4201 gcgccaagcc ggaccagatt ggcatcatca cgccctacga gggccagcgc tcctacctgg
     4261 tgcagtacat gcagttcagc ggctccctgc acaccaagct ctaccaggag gtggagatcg
     4321 ccagtgtgga cgcctttcag ggacgcgaga aggacttcat catcctgtcc tgtgtgcggg
     4381 ccaacgagca ccaaggcatt ggctttttaa atgaccccag gcgtctgaac gtggccctga
     4441 ccagagcaag gtatggcgtc atcattgtgg gcaacccgaa ggcactatca aagcagccgc
     4501 tctggaacca cctgctgaac tactataagg agcagaaggt gctggtggag gggccgctca
     4561 acaacctgcg tgagagcctc atgcagttca gcaagccacg gaagctggtc aacactatca
     4621 acccgggagc ccgcttcatg accacagcca tgtatgatgc ccgggaggcc atcatcccag
     4681 gctccgtcta tgatcggagc agccagggcc ggccttccag catgtacttc cagacccatg
     4741 accagattgg catgatcagt gccggcccta gccacgtggc tgccatgaac attcccatcc
     4801 ccttcaacct ggtcatgcca cccatgccac cgcctggcta ttttggacaa gccaacgggc
     4861 ctgctgcagg gcgaggcacc ccgaaaggca agactggtcg tgggggacgc cagaagaacc
     4921 gctttgggct tcctggaccc agccagacta acctccccaa cagccaagcc agccaggatg
     4981 tggcgtcaca gcccttctct cagggcgccc tgacgcaggg ctacatctcc atgagccagc
     5041 cttcccagat gagccagccc ggcctctccc agccggagct gtcccaggac agttaccttg
     5101 gtgacgagtt taaatcacaa atcgacgtgg cgctctcaca ggactccacg taccagggag
     5161 agcgggctta ccagcatggc ggggtgacgg ggctgtccca gtattaaaag gtggcggcgg
     5221 aagagctaaa gagtcgaccc gggcggccgc tcgagtctag agggcccgtt taaacccgct
     5281 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5341 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5401 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     5461 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     5521 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     5581 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5641 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5701 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5761 accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt
     5821 cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt
     5881 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     5941 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     6001 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     6061 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     6121 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     6181 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     6241 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     6301 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     6361 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     6421 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     6481 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     6541 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     6601 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     6661 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     6721 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     6781 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     6841 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt
     6901 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6961 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7021 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7081 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7141 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9301 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9361 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     9421 ccctgcttgt gtgttggagg tcgctg
//
