LOCUS       sequence-269866-        9434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus 
            and used with the Flp-In T-Rex System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pcDNA5 FRT-TO-EGFP-UPF1-DEAA
FEATURES             Location/Qualifiers
     source          1..9434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     629..649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    680..698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    701..719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     729..753
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          876..1008
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     991..1010
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1053..1071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1087..1096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1093..1095,1096..1098,1099..1809)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1093..1095,1096..1098,1099..1809)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1138..1159)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1399..1418)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1746..1767
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5246..5263)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5252..5476
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5609..5628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5760..5807
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5815..6837
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5815..6837
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6967..7088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7004..7023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7058..7077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7137..7153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7137..7153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7150..7172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7161..7177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7185..7191,7192..7209,7210..7215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7230..7251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7368..7385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7539..8127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7619..7638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8298..9089,9090..9158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8921..8940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9159..9263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9338..9357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
       61 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      121 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      481 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      601 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      661 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat
      721 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      781 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag
      841 cttcttgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      901 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      961 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
     1021 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctagcgcta
     1081 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1141 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1201 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1261 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1321 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1381 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1441 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1501 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1561 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1621 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1681 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1741 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1801 ctgtacaagc ttagcgtgga ggcgtacggg cccagctcgc agactctcac tttcctggac
     1861 acggaggagg ccgagctgct tggcgccgac acacagggct ccgagttcga gttcaccgac
     1921 tttactcttc ctagccagac gcagacgccc cccggcggcc ccggcggccc gggcggtggc
     1981 ggcgcgggag gcccgggcgg cgcgggcgcg ggcgctgcgg cgggacagct cgacgcgcag
     2041 gttgggcccg aaggcatcct gcagaacggg gctgtggacg acagtgtagc caagaccagc
     2101 cagttgttgg ctgagttgaa cttcgaggaa gatgaagaag acacctatta cacgaaggac
     2161 ctccccatac acgcctgcag ttactgtgga atacacgatc ctgcctgcgt ggtttactgt
     2221 aataccagca agaagtggtt ctgcaacgga cgtggaaata cttctggcag ccacattgta
     2281 aatcaccttg tgagggcaaa atgcaaagag gtgaccctgc acaaggacgg gcccctgggg
     2341 gagacagtcc tggagtgcta caactgcggc tgtcgcaacg tcttcctcct cggcttcatc
     2401 ccggccaaag ctgactcagt ggtggtgctg ctgtgcaggc agccctgtgc cagccagagc
     2461 agcctcaagg acatcaactg ggacagctcg cagtggcagc cgctgatcca ggaccgctgc
     2521 ttcctgtcct ggctggtcaa gatcccctcc gagcaggagc agctgcgggc acgccagatc
     2581 acggcacagc agatcaacaa gctggaggag ctgtggaagg aaaacccttc tgccacgctg
     2641 gaggacctgg agaagccggg ggtggacgag gagccgcagc atgtcctcct gcggtacgag
     2701 gacgcctacc agtaccagaa catattcggg cccctggtca agctggaggc cgactacgac
     2761 aagaagctga aggagtccca gactcaagat aacatcactg tcaggtggga cctgggcctt
     2821 aacaagaaga gaatcgccta cttcactttg cccaagactg actctgacat gcggctcatg
     2881 cagggggatg agatatgcct gcggtacaaa ggggaccttg cgcccctgtg gaaagggatc
     2941 ggccacgtca tcaaggtccc tgataattat ggcgatgaga tcgccattga gctgcggagc
     3001 agcgtgggtg cacctgtgga ggtgactcac aacttccagg tggattttgt gtggaagtcg
     3061 acctcctttg acaggatgca gagcgcattg aaaacgtttg ccgtggatga gacctcggtg
     3121 tctggctaca tctaccacaa gctgttgggc cacgaggtgg aggacgtaat catcaagtgc
     3181 cagctgccca agcgcttcac ggcgcagggc ctccccgacc tcaaccactc ccaggtttat
     3241 gccgtgaaga ctgtgctgca aagaccactg agcctgatcc agggcccgcc aggcacgggg
     3301 aagacggtga cgtcggccac catcgtctac cacctggccc ggcaaggcaa cgggccggtg
     3361 ctggtgtgtg ctccgagcaa catcgccgtg gaccagctaa cggagaagat ccaccagacg
     3421 gggctaaagg tcgtgcgcct ctgcgccaag agccgtgagg ccatcgactc cccggtgtct
     3481 tttctggccc tgcacaacca gatcaggaac atggacagca tgcctgagct gcagaagctg
     3541 cagcagctga aagacgagac tggggagctg tcgtctgccg acgagaagcg gtaccgggcc
     3601 ttgaagcgca ccgcagagag agagctgctg atgaacgcag atgtcatctg ctgcacatgt
     3661 gtgggcgccg gtgacccgag gctggccaag atgcagttcc gctccatttt aatcgccgca
     3721 agcacccagg ccaccgagcc ggagtgcatg gttcccgtgg tcctcggggc caagcagctg
     3781 atccttgtag gcgaccactg ccagctgggc ccagtggtga tgtgcaagaa ggcggccaag
     3841 gccgggctgt cacagtcgct cttcgagcgc ctggtggtgc tgggcatccg gcccatccgc
     3901 ctgcaggtcc agtaccggat gcaccctgca ctcagcgcct tcccatccaa catcttctac
     3961 gagggctccc tccagaatgg tgtcactgca gcggatcgtg tgaagaaggg atttgacttc
     4021 cagtggcccc aacccgataa accgatgttc ttctacgtga cccagggcca agaggagatt
     4081 gccagctcgg gcacctccta cctgaacagg accgaggctg cgaacgtgga gaagatcacc
     4141 acgaagttgc tgaaggcagg cgccaagccg gaccagattg gcatcatcac gccctacgag
     4201 ggccagcgct cctacctggt gcagtacatg cagttcagcg gctccctgca caccaagctc
     4261 taccaggagg tggagatcgc cagtgtggac gcctttcagg gacgcgagaa ggacttcatc
     4321 atcctgtcct gtgtgcgggc caacgagcac caaggcattg gctttttaaa tgaccccagg
     4381 cgtctgaacg tggccctgac cagagcaagg tatggcgtca tcattgtggg caacccgaag
     4441 gcactatcaa agcagccgct ctggaaccac ctgctgaact actataagga gcagaaggtg
     4501 ctggtggagg ggccgctcaa caacctgcgt gagagcctca tgcagttcag caagccacgg
     4561 aagctggtca acactatcaa cccgggagcc cgcttcatga ccacagccat gtatgatgcc
     4621 cgggaggcca tcatcccagg ctccgtctat gatcggagca gccagggccg gccttccagc
     4681 atgtacttcc agacccatga ccagattggc atgatcagtg ccggccctag ccacgtggct
     4741 gccatgaaca ttcccatccc cttcaacctg gtcatgccac ccatgccacc gcctggctat
     4801 tttggacaag ccaacgggcc tgctgcaggg cgaggcaccc cgaaaggcaa gactggtcgt
     4861 gggggacgcc agaagaaccg ctttgggctt cctggaccca gccagactaa cctccccaac
     4921 agccaagcca gccaggatgt ggcgtcacag cccttctctc agggcgccct gacgcagggc
     4981 tacatctcca tgagccagcc ttcccagatg agccagcccg gcctctccca gccggagctg
     5041 tcccaggaca gttaccttgg tgacgagttt aaatcacaaa tcgacgtggc gctctcacag
     5101 gactccacgt accagggaga gcgggcttac cagcatggcg gggtgacggg gctgtcccag
     5161 tattaaaagg tggcggcgga agagctaaag agtcgacccg ggcggccgct cgagtctaga
     5221 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5281 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5341 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5401 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5461 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5521 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5581 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5641 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5701 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     5761 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     5821 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     5881 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     5941 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     6001 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     6061 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     6121 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     6181 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     6241 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     6301 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     6361 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     6421 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     6481 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     6541 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     6601 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     6661 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     6721 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     6781 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     6841 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6901 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6961 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7081 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7201 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9301 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     9361 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     9421 aaatttaagc taca
//
