LOCUS       sequence-269873-       11724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (Y84P)
FEATURES             Location/Qualifiers
     source          1..11724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1376..1394)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1462..1566
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1567..1635,1636..2427)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1785..1804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2598..3186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3087..3106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3617..3762
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3805..4842
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4840..4859)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4884..4932
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5586..5965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5966..6169
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6120..6140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6166..6190
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6272..6301
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(7349..7351,7352..7810)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(7391..7412)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7652..7671)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7833..7967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7857..7876)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7911..7930
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8110..8132)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8692..10481
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcctccacct cctgctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc
       61 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
      121 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctsc
      181 ccctcctgcc ccymctcccg cctcctcctc ctcctcctcc ccctcctccc cctgctactc
      241 ctgcccctcc tgctcctscc cctcctgctc ctgcccctcc ygcwcctccc cctcctgccc
      301 ctcctgcccc tcctcctgct cctgcccctc ctsctcctsc ccctcctgcc cctcctgccc
      361 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
      421 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
      481 cccctcctcc tccccctctt cchccscccc tccctytycc ccccctccgc cttggccctc
      541 ctcctgctcc cccccccccc cctcgctcct scccctcctg cccctcctgc ccctcctgcc
      601 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcccgct
      661 cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact tggacgtttt
      721 tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc cggggctcct
      781 ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt atcaccccct
      841 cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc cttctctcct
      901 aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac taaaagagat
      961 caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac tcctgccccc
     1021 tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat ccaggtctga
     1081 aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca gcccggaaaa
     1141 ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct caaattcctc
     1201 gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct cttcctcttc
     1261 aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg cggcctgtga
     1321 ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc
     1381 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1441 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1501 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1561 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1681 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1741 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1801 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1861 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1921 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1981 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2101 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2161 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2221 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2401 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2461 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2521 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3181 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc
     3241 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     3301 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
     3361 gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga
     3421 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     3481 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt
     3541 catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca
     3601 tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa
     3661 cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg
     3721 tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc
     3781 gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     3841 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     3901 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     3961 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     4021 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     4081 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     4141 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     4201 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     4261 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     4321 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     4381 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     4441 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     4501 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     4561 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     4621 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     4681 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     4741 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     4801 cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat
     4861 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     4921 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     4981 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     5041 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca
     5101 ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt
     5161 tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg
     5221 gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct
     5281 gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc
     5341 tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
     5401 attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca
     5461 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca
     5521 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
     5581 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     5641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5821 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
     5881 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
     5941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
     6001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6061 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
     6121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     6181 ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgaaga ccatcatcgc
     6241 cctgagctac atcttctgcc tggtgttcgc cgagcagaaa ttgatcagcg aggaggacct
     6301 gggcagccct ggaggcagtt caggaggcgg atctcactcc atgaggtatt tcttcacatc
     6361 cgtgtcccgg cccggccgcg gggagccccg cttcatcgca gtgggctacg tggacgacac
     6421 gcagttcgtg cggttcgaca gcgacgccgc gagccagagg atggagccgc gggcgccgtg
     6481 gatagagcag gagggtccgg agtattggga cggggagaca cggaaagtga aggcccactc
     6541 acagactcac cgagtggacc tggggaccct gcgcggcccc tacaaccaga gcgaggccgg
     6601 ttctcacacc gtccagagga tgtatggctg cgacgtgggg tcggactggc gcttcctccg
     6661 cgggtaccac cagtacgcct acgacggcaa ggattacatc gccctgaaag aggacctgcg
     6721 ctcttggacc gcggcggaca tggcagctca gaccaccaag cacaagtggg aggcggccca
     6781 tgtggcggag cagttgagag cctacctgga gggcacgtgc gtggagtggc tccgcagata
     6841 cctggagaac gggaaggaga cgctgcagcg tacggacgcc cccaaaacgc atatgactca
     6901 ccacgctgtc tctgaccatg aagccaccct gaggtgctgg gccctgagct tctaccctgc
     6961 ggagatcaca ctgacctggc agcgggatgg ggaggaccag acccaggaca cggagctcgt
     7021 ggagaccagg cctgcagggg atggaacctt ccagaagtgg gcggctgtgg tggtgccttc
     7081 tggacaggag cagagataca cctgccatgt gcagcatgag ggtttgccca agcccctcac
     7141 cctgagatgg gagccgtctt cccagcccac catccccatc gtgggcatca ttgctggcct
     7201 ggttctcttt ggagctgtga tcactggagc tgtggtcgct gctgtgatgt ggaggaggaa
     7261 gagctcagat agaaaaggag ggagctactc tcaggctgca agcagtgaca gtgcccaggg
     7321 ctctgatgtg tctctcacag cttgtaaagt gagcaagggc gaggagctgt tcaccggggt
     7381 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7441 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     7501 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
     7561 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7621 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7681 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7741 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     7801 tctgaccgcc taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg
     7861 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7921 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     7981 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     8041 acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga
     8101 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8161 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     8221 agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
     8281 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     8341 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     8401 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     8461 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     8521 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     8581 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     8641 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     8701 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     8761 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     8821 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     8881 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     8941 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     9001 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     9061 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     9121 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     9181 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     9241 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     9301 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     9361 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     9421 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     9481 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     9541 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     9601 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     9661 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     9721 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     9781 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     9841 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     9901 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     9961 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
    10021 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
    10081 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
    10141 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
    10201 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
    10261 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    10321 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
    10381 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
    10441 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
    10501 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
    10561 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
    10621 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
    10681 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
    10741 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
    10801 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
    10861 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
    10921 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
    10981 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
    11041 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
    11101 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
    11161 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
    11221 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
    11281 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
    11341 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
    11401 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
    11461 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
    11521 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
    11581 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
    11641 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
    11701 gcctccactg cctcctcgac cccg
//
