LOCUS       sequence-269880-       13459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, 
            Procko E
  TITLE     Engineered receptors for human cytomegalovirus that are orthogonal 
            to normal human biology.
  JOURNAL   PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 
            10.1371/journal.ppat.1008647. eCollection 2020 Jun.
  PUBMED    32559251
REFERENCE   2  (bases 1 to 13459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-06-19"; volume: "16(6):e1008647. doi"; pages: 
            " 10.1371/journal.ppat.1008647. eCollection 2020 Jun"
COMMENT     Sequence Label: pCEP4-myc-PDGFRalpha-Y206S
FEATURES             Location/Qualifiers
     source          1..13459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        703..1082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1083..1286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1237..1257
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1283..1307
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1389..1418
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4666..4800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4690..4709)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4744..4763
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4943..4965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5525..7314
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9952..9970)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10038..10142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10143..10211,10212..11003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10361..10380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11174..11762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11663..11682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        12193..12338
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             12381..13418
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(13416..13435)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
       61 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
      121 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
      181 gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg
      241 ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg
      301 gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg
      361 accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc
      421 cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg
      481 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
      541 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      601 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      661 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      721 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      781 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      841 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      901 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      961 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1021 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1081 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
     1141 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1201 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
     1261 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg
     1321 taccagctgc tagcgccacc atgaagacca tcatcgccct gagctacatc ttctgcctgg
     1381 tgttcgccga gcagaaattg atcagcgagg aggacctggg cagccctgga ggcgcctctg
     1441 gccagctttc attaccctct atccttccaa atgaaaatga aaaggttgtg cagctgaatt
     1501 catccttttc tctgagatgc tttggggaga gtgaagtgag ctggcagtac cccatgtctg
     1561 aagaagagag ctccgatgtg gaaatcagaa atgaagaaaa caacagcggc ctttttgtga
     1621 cggtcttgga agtgagcagt gcctcggcgg cccacacagg gttgtacact tgctattaca
     1681 accacactca gacagaagag aatgagcttg aaggcaggca catttacatc tatgtgccag
     1741 acccagatgt agcctttgta cctctaggaa tgacggatta tttagtcatc gtggaggatg
     1801 atgattctgc cattatacct tgtcgcacaa ctgatcccga gactcctgta accttacaca
     1861 acagtgaggg ggtggtacct gcctcctatg acagcagaca gggctttaat gggaccttca
     1921 ctgtagggcc ctatatctgt gaggccaccg tcaaaggaaa gaagttccag accatcccat
     1981 ttaatgtttc tgctttaaaa gcaacatcag agctggatct agaaatggaa gctcttaaaa
     2041 ccgtgtataa gtcaggggaa acgattgtgg tcacctgtgc tgtttttaac aatgaggtgg
     2101 ttgaccttca atggacttac cctggagaag tgaaaggcaa aggcatcaca atgctggaag
     2161 aaatcaaagt cccatccatc aaattggtgt acactttgac ggtccccgag gccacggtga
     2221 aagacagtgg agattacgaa tgtgctgccc gccaggctac cagggaggtc aaagaaatga
     2281 agaaagtcac tatttctgtc catgagaaag gtttcattga aatcaaaccc accttcagcc
     2341 agttggaagc tgtcaacctg catgaagtca aacattttgt tgtagaggtg cgggcctacc
     2401 cacctcccag gatatcctgg ctgaaaaaca atctgactct gattgaaaat ctcactgaga
     2461 tcaccactga tgtggaaaag attcaggaaa taaggtatcg aagcaaatta aagctgatcc
     2521 gtgctaagga agaagacagt ggccattata ctattgtagc tcaaaatgaa gatgctgtga
     2581 agagctatac ttttgaactg ttaactcaag ttccttcatc cattctggac ttggtcgatg
     2641 atcaccatgg ctcaactggg ggacagaccg tacggtgcac agctgaaggc acgccgcttc
     2701 ctgatattga gtggatgata tgcaaagata ttaagaaatg taataatgaa acttcctgga
     2761 ctattttggc caacaatgtc tcaaacatca tcacggagat ccactcccga gacaggagta
     2821 ccgtggaggg ccgtgtgact ttcgccaaag tggaggagac catcgccgtg cgatgcctgg
     2881 ctaagaatct ccttggagct gagaaccgag agctgaagct ggtggctccc accctgcgtt
     2941 ctgaactcac ggtggctgct gcagtcctgg tgctgttggt gattgtgatc atctcactta
     3001 ttgtcctggt tgtcatttgg aaacagaaac cgaggtatga aattcgctgg agggtcattg
     3061 aatcaatcag cccggatgga catgaatata tttatgtgga cccgatgcag ctgccttatg
     3121 actcaagatg ggagtttcca agagatggac tagtgcttgg tcgggtcttg gggtctggag
     3181 cgtttgggaa ggtggttgaa ggaacagcct atggattaag ccggtcccaa cctgtcatga
     3241 aagttgcagt gaagatgcta aaacccacgg ccagatccag tgaaaaacaa gctctcatgt
     3301 ctgaactgaa gataatgact cacctggggc cacatttgaa cattgtaaac ttgctgggag
     3361 cctgcaccaa gtcaggcccc atttacatca tcacagagta ttgcttctat ggagatttgg
     3421 tcaactattt gcataagaat agggatagct tcctgagcca ccacccagag aagccaaaga
     3481 aagagctgga tatctttgga ttgaaccctg ctgatgaaag cacacggagc tatgttattt
     3541 tatcttttga aaacaatggt gactacatgg acatgaagca ggctgatact acacagtatg
     3601 tccccatgct agaaaggaaa gaggtttcta aatattccga catccagaga tcactctatg
     3661 atcgtccagc ctcatataag aagaaatcta tgttagactc agaagtcaaa aacctccttt
     3721 cagatgataa ctcagaaggc cttactttat tggatttgtt gagcttcacc tatcaagttg
     3781 cccgaggaat ggagtttttg gcttcaaaaa attgtgtcca ccgtgatctg gctgctcgca
     3841 acgtcctcct ggcacaagga aaaattgtga agatctgtga ctttggcctg gccagagaca
     3901 tcatgcatga ttcgaactat gtgtcgaaag gcagtacctt tctgcccgtg aagtggatgg
     3961 ctcctgagag catctttgac aacctctaca ccacactgag tgatgtctgg tcttatggca
     4021 ttctgctctg ggagatcttt tcccttggtg gcacccctta ccccggcatg atggtggatt
     4081 ctactttcta caataagatc aagagtgggt accggatggc caagcctgac cacgctacca
     4141 gtgaagtcta cgagatcatg gtgaaatgct ggaacagtga gccggagaag agaccctcct
     4201 tttaccacct gagtgagatt gtggagaatc tgctgcctgg acaatataaa aagagttatg
     4261 aaaaaattca cctggacttc ctgaagagtg accatcctgc tgtggcacgc atgcgtgtgg
     4321 actcagacaa tgcatacatt ggtgtcacct acaaaaacga ggaagacaag ctgaaggact
     4381 gggagggtgg tctggatgag cagagactga gcgctgacag tggctacatc attcctctgc
     4441 ctgacattga ccctgtccct gaggaggagg acctgggcaa gaggaacaga cacagctcgc
     4501 agacctctga agagagtgcc attgagacgg gttccagcag ttccaccttc atcaagagag
     4561 aggacgagac cattgaagac atcgacatga tggacgacat cggcatagac tcttcagacc
     4621 tggtggaaga cagcttcctg taatgactcg aggccggcaa ggccggatcc agacatgata
     4681 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     4741 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4801 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     4861 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc
     4921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5041 ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc cggacgaact
     5101 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     5161 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     5221 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     5281 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     5341 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     5401 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     5461 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     5521 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     5581 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     5641 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     5701 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     5761 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     5821 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     5881 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     5941 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     6001 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     6061 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     6121 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     6181 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     6241 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     6301 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     6361 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     6421 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     6481 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     6541 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     6601 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     6661 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6721 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     6781 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     6841 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     6901 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     6961 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     7021 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     7081 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     7141 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     7201 ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc
     7261 atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt
     7321 gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct
     7381 caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt
     7441 gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag
     7501 acagatggcc agaaatacac caacagcacg catgatgtct actggggatt tattctttag
     7561 tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact
     7621 cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac
     7681 cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt
     7741 caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt
     7801 ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat
     7861 aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa
     7921 aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc
     7981 ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc
     8041 cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa
     8101 tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt
     8161 ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt
     8221 catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac
     8281 cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac
     8341 ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg
     8401 gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg
     8461 ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac
     8521 ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc
     8581 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     8641 ctgcccctcc tgctcctgcc cctcctgccc ctccygctcc cscctctacc gcactctccg
     8701 ctcgccatcg tcctcctcac tccagtagtc cctcgctgcc ccttcccccc cccctccccc
     8761 cccgcccycc cscccyyccc cmcccccccc cycccscccc tccccccccc cscccccgmc
     8821 csccyccyck mcctccsccc ccccckccwc ctcctccccc tccwccccct ccycctcctc
     8881 ctcctccrcc ccsgcctccc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     8941 cccctcctgc ccctcctcct gctcctgccc ctcctcctcc tcctccycct ccyccccctc
     9001 cyccyccycc yccccctccc cccccyccyc cyccyccycc tccccctccc cctcctccyc
     9061 ctcctcctcc tcctccycct cctcctcctc ctcctcctcc tccycctcct ccyccycctc
     9121 ctcctcctcc ccctcctcct cctcctcccc ctcctccccc tcctccycct cctscccctc
     9181 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
     9241 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
     9301 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
     9361 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
     9421 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
     9481 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
     9541 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
     9601 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
     9661 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
     9721 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
     9781 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
     9841 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     9901 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
     9961 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
    10021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10381 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10681 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11281 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    11341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    11461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc
    11821 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
    11881 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
    11941 gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc
    12001 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
    12061 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc
    12121 cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
    12181 tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
    12241 cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg
    12301 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
    12361 atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
    12421 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
    12481 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
    12541 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
    12601 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
    12661 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
    12721 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
    12781 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
    12841 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
    12901 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
    12961 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
    13021 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
    13081 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
    13141 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
    13201 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
    13261 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
    13321 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
    13381 gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg
    13441 gaaggaaccc gcgctatga
//
