LOCUS       sequence-269892-       10976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-B*07:02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B7
FEATURES             Location/Qualifiers
     source          1..10976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1783..1917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1807..1826)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1861..1880
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2060..2082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6767..6785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6853..6957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6958..7026,7027..7818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7176..7195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7989..8577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8478..8497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9008..9153
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9196..10233
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10231..10250)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10275..10323
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cagttcagga ggcggatctc attctatgag atatttctac acctccgtgt
      781 cccggcccgg ccgcggggag ccccgcttca tctcagtggg ctacgtggac gacacccagt
      841 tcgtgaggtt cgacagcgac gccgcgagtc cgagagagga gccgcgggcg ccgtggatag
      901 agcaggaggg gccggagtat tgggaccgga acacacagat ctacaaggcc caggcacaga
      961 ctgaccgaga gagcctgcgg aacctgcgcg gctactacaa ccagagcgag gccgggtctc
     1021 acaccctcca gagcatgtac ggctgcgacg tggggccgga cgggcgcctc ctccgcgggc
     1081 atgaccagta cgcctacgac ggcaaggatt acatcgccct gaacgaggac ctgcgctcct
     1141 ggaccgccgc ggacacggcg gctcagatca cccagcgcaa gtgggaggcg gcccgtgagg
     1201 cggagcagcg gagagcctac ctggagggcg agtgcgtgga gtggctccgc agatacctgg
     1261 agaacgggaa ggacaagctg gagcgcgctg accccccaaa gacacacgtg acccaccacc
     1321 ccatctctga ccatgaggcc accctgaggt gctgggccct gggtttctac cctgcggaga
     1381 tcacactgac ctggcagcgg gatggcgagg accaaactca ggacactgag cttgtggaga
     1441 ccagaccagc aggagataga accttccaga agtgggcagc tgtggtggtg ccttctggag
     1501 aagagcagag atacacatgc catgtacagc atgaggggct gccgaagccc ctcaccctga
     1561 gatgggagcc gtcttcccag tccacagtgc ccatcgttgg aatcgttgca ggactggcag
     1621 ttctcgccgt agtggttata ggagcagtgg tcgctgctgt tatgtgtcgc aggaaaagtt
     1681 caggcggcaa gggaggatct tactcacaag cggcgtgctc cgatagcgca caaggctcag
     1741 atgtttctct gacggcatga taactcgagg ccggcaaggc cggatccaga catgataaga
     1801 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1861 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1921 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1981 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt
     2041 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2101 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2161 gtcggggcgc agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa
     2221 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     2281 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     2341 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     2401 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     2461 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     2521 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     2581 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     2641 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     2701 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     2761 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     2821 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     2881 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     2941 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     3001 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     3061 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     3121 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     3181 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     3241 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     3301 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     3361 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     3421 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     3481 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     3541 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     3601 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     3661 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     3721 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     3781 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     3841 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     3901 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     3961 cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag catatgatac
     4021 ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga
     4081 attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg
     4141 ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca
     4201 acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc
     4261 cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac
     4321 gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct tttaatacga
     4381 ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca
     4441 cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt
     4501 ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg
     4561 caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca
     4621 aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca
     4681 ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga
     4741 cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct
     4801 ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc
     4861 ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct
     4921 tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc
     4981 cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc
     5041 gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct
     5101 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     5161 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     5221 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     5281 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     5341 cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     5401 gccccyccts cccctcctcc tcctcccccc cccsccccyc cyscycctsc ccctcctgcc
     5461 cctcctgctc ctgcccctcc tgcccctcct cctsctccyc ctcctcctcc scctcctcct
     5521 rctcctkccc ctcctccbcc tcctgctcct gcccctcctr cccctcctgc ycctgcccct
     5581 cctccscctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     5641 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctsc tccccccccc
     5701 cctgctccts cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     5761 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     5821 gctcctgccc ctcctccccc tccycccccy ccycctccyc cyccscctcc ccccccbcct
     5881 ccccckccyc ccccscctcc tccycctccc cctcctcctc ctcctccycc tcctcctcct
     5941 cctscccctc ctcctgctcc tgcccctcct gcccctccts cccctcctgc ccctcctcct
     6001 gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct
     6061 gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc
     6121 cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg
     6181 cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga
     6241 ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt
     6301 ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca
     6361 tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc
     6421 ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc
     6481 atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg
     6541 aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt
     6601 tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc
     6661 agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct
     6721 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc
     6781 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7081 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7141 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7201 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7261 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7321 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7381 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7441 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7501 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7561 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7621 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7681 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7741 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7801 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8101 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga
     8641 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     8701 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     8761 acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     8821 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     8881 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt
     8941 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
     9001 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat
     9061 gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
     9121 gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatccc
     9181 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     9241 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     9301 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     9361 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     9421 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     9481 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     9541 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     9601 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     9661 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     9721 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     9781 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     9841 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     9901 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     9961 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
    10021 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
    10081 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
    10141 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
    10201 cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    10261 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
    10321 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
    10381 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
    10441 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg
    10501 tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt
    10561 gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga
    10621 tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc
    10681 aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc
    10741 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
    10801 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
    10861 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
    10921 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgtt
//
