LOCUS       sequence-269932-        5419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ABCC8 exon 27 wild type for the analysis of splicing 
            variants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Lago-Docampo M, Tenorio J, Hernandez-Gonzalez I, Perez-Olivares C, 
            Escribano-Subias P, Pousada G, Baloira A, Arenas M, Lapunzina P, 
            Valverde D
  TITLE     Characterization of rare ABCC8 variants identified in Spanish 
            pulmonary arterial hypertension patients.
  JOURNAL   Sci Rep. 2020 Sep 15;10(1):15135. doi: 10.1038/s41598-020-72089-1.
  PUBMED    32934261
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-15"; volume: "10(1):15135. doi"; pages: " 
            10.1038/s41598-020-72089-1"
COMMENT     Sequence Label: pSPL3-ABCC8-27
FEATURES             Location/Qualifiers
     source          1..5419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1555..1788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1973..2017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    2332..2466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2356..2375)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2410..2429
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3140..3244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3245..3313,3314..4105)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3463..3482)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4276..4864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4765..4784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5018..5035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5105..5125)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(5110..5419,1..20)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5290..5419,1..6)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaggcctagg cttttgcaaa aagcttggac tgtgtttact tgcaatcccc caaaacagac
       61 agaatggtgc atctgtccag tgaggagaag tctgcggtca ctgccctgtg gggcaaggtg
      121 aatgtggaag aagttggtgg tgaggccctg ggcaggctgc tggttgtcta cccatggacc
      181 cagaggttct tcgagtcctt tggggacctg tcctctgcaa atgctgttat gaacaatcct
      241 aaggtgaagg ctcatggcaa gaaggtgctg gctgccttca gtgagggtct gagtcacctg
      301 gacaacctca aaggcacctt tgctaagctg agtgaactgc actgtgacaa gctgcacgct
      361 ctagagtcga cccagcagta agtaatacat gtaatgcaac ctatacaaat agcaatagta
      421 gcattagtag tagcaataat aatagcaata gttgtgtggt ccatagtaat catagaatat
      481 aggaaaatat taagacaaag aaaaatagac aggttaattg atagactaat agaaagagca
      541 gaagacagtg gcaatgagag tgaaggagaa atatcagcac ttgtggagat gggggtggag
      601 atggggcacc atgctccttg ggatgttgat gatctgtagt gctacagaaa aattgtgggt
      661 cacagtctat tatggggtag ggatcaccag aattctggag ctcgagaaat tggcagagga
      721 tgccagaccc atgaatgact ccagagacac ttacatgaaa taattaacta tctcattcag
      781 taaacaggaa gtaaatcaat ggacaaatga gtaactcttc ctctggctgc ctcttaggtt
      841 ttttgagacc acgccccttg ggagcatcct gaacagattt tcatctgact gtaacaccat
      901 cgaccaggta cagaggaccg tgccacacta tccccccttc cagagacacc tcggacccct
      961 gggggcttct cctgtctgag gctgggttcc ccagatcagg tgatcacagc cctctgggaa
     1021 gaagccagtg tccccgatcc gattagctag caaattaaga gaacaatttg gaaataataa
     1081 aacaataatc tttaagcaat cctcaggagg ggacccagaa attgtaacgc acagttttaa
     1141 ttgtggaggg gaatttttct actgtaattc aacacaactg tttaatagta cttggtttaa
     1201 tagtacttgg agtactgaag ggtcaaataa cactgaagga agtgacacaa tcacactccc
     1261 atgcagaata aaacaattta taaacatgtg gcaggaagta ggaaaagcaa tgtatgcccc
     1321 tcccatcagc ggacaaatta gatgttcatc aaatattaca gggctgctat taacaagaga
     1381 tggtggtaat aacaacaatg ggtccgagat cttcagacct ggaggaggag atatgaggga
     1441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2101 agtttttgct gtactttctg tagtgaatag agttaggcag ggatattcac cattatcgtt
     2161 tcagacctgg agatctcccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2221 gagagagaga cagagacaga tccattcgac caattcactc ctcaggtgca ggctgcctat
     2281 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatccagac
     2341 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2401 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2461 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     2521 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccccaggaa
     2581 gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt ccaatcatag
     2641 gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa aaggaaattg
     2701 ggtaggggtt tttcacagac cgctttctaa gggtaatttt aaaatatctg ggaagtccct
     2761 tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca gcagaaacat
     2821 acaagctgtc agctttgcac aagggcccaa caccctgctc atcaagaagc actgtggttg
     2881 ctgtgttagt aatgtgcaaa acaggaggca cattttcccc acctgtgtag gttccaaaat
     2941 atctagtgtt ttcattttta cttggatcag gaacccagca ctccactgga taagcattat
     3001 ccttatccaa aacagccttg tggtcagtgt tcatctgctg actgtcaact gtagcatttt
     3061 ttggggttac agtttgagca ggatatttgg tcctgtagtt tgctaacaca ccccaggtgg
     3121 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3181 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3241 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3301 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3361 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3421 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3481 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3541 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3601 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3661 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3721 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3781 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3841 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3901 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3961 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4021 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4081 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4141 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4201 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4261 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4321 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4381 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4441 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4501 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4561 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4621 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4681 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4741 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4801 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4861 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4921 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4981 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5041 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5101 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5161 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5221 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5281 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5341 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5401 tagtgaggag gcttttttg
//
