LOCUS       sequence-269935-        5736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ABCC8 exon 2 wild type for the analysis of splicing 
            variants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Lago-Docampo M, Tenorio J, Hernandez-Gonzalez I, Perez-Olivares C, 
            Escribano-Subias P, Pousada G, Baloira A, Arenas M, Lapunzina P, 
            Valverde D
  TITLE     Characterization of rare ABCC8 variants identified in Spanish 
            pulmonary arterial hypertension patients.
  JOURNAL   Sci Rep. 2020 Sep 15;10(1):15135. doi: 10.1038/s41598-020-72089-1.
  PUBMED    32934261
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-15"; volume: "10(1):15135. doi"; pages: " 
            10.1038/s41598-020-72089-1"
COMMENT     Sequence Label: pSPL3-ABCC8-2
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..61)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        46..375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      226..361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2227..2460
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2645..2689
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3004..3138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3028..3047)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3082..3101
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3812..3916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3917..3985,3986..4777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4135..4154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4948..5536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5437..5456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5690..5707
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
       61 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      361 ctaggctttt gcaaaaagct tggactgtgt ttacttgcaa tcccccaaaa cagacagaat
      421 ggtgcatctg tccagtgagg agaagtctgc ggtcactgcc ctgtggggca aggtgaatgt
      481 ggaagaagtt ggtggtgagg ccctgggcag gctgctggtt gtctacccat ggacccagag
      541 gttcttcgag tcctttgggg acctgtcctc tgcaaatgct gttatgaaca atcctaaggt
      601 gaaggctcat ggcaagaagg tgctggctgc cttcagtgag ggtctgagtc acctggacaa
      661 cctcaaaggc acctttgcta agctgagtga actgcactgt gacaagctgc acgctctaga
      721 gtcgacccag cagtaagtaa tacatgtaat gcaacctata caaatagcaa tagtagcatt
      781 agtagtagca ataataatag caatagttgt gtggtccata gtaatcatag aatataggaa
      841 aatattaaga caaagaaaaa tagacaggtt aattgataga ctaatagaaa gagcagaaga
      901 cagtggcaat gagagtgaag gagaaatatc agcacttgtg gagatggggg tggagatggg
      961 gcaccatgct ccttgggatg ttgatgatct gtagtgctac agaaaaattg tgggtcacag
     1021 tctattatgg ggtagggatc accagaattc tggagctcga gggggaggta ccagcatagg
     1081 accaggctga atgagttgga cgtttagttt gtgtgtacca gccttgggga tcctcatgtg
     1141 taggtgtcac cctcaccatg gacccaatgc ccaagcacct ttctggggtt catgcaccct
     1201 cttcctcctc caagtgggca ggcatcctgc ctctgactgt ctctccctgc aggatgggga
     1261 agtcagagct ccaaggtgca catccaccac agcacatggc ttcatttccc tgggcacaac
     1321 ctgcggtgga tcctgacctt catgctgctc ttcgtcctgg tgtgtgagat tgcagagggc
     1381 atcctgtctg atgggtgagt gactgtctga ggactcagga aagtgaatct gctccagggt
     1441 acttcctgaa aggcccaagg agatcctagc tcacctatat tccacaagcc catgtctttt
     1501 gttactacat ccagcagctc agtgtcttct atgtgccaac ctctctgagc tgggcactgg
     1561 acgcaagtga gtcccaggcc ctgccatcag tccaggagag cagagggaac acggacctgc
     1621 atgccaagtc ccagtgaaat aatgctagag cctattagaa tgccacaact ccagtgaaaa
     1681 atgtctggcc tcttctacaa cctgccggag ttaccttgct agcaaattaa gagaacaatt
     1741 tggaaataat aaaacaataa tctttaagca atcctcagga ggggacccag aaattgtaac
     1801 gcacagtttt aattgtggag gggaattttt ctactgtaat tcaacacaac tgtttaatag
     1861 tacttggttt aatagtactt ggagtactga agggtcaaat aacactgaag gaagtgacac
     1921 aatcacactc ccatgcagaa taaaacaatt tataaacatg tggcaggaag taggaaaagc
     1981 aatgtatgcc cctcccatca gcggacaaat tagatgttca tcaaatatta cagggctgct
     2041 attaacaaga gatggtggta ataacaacaa tgggtccgag atcttcagac ctggaggagg
     2101 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     2161 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     2221 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     2281 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     2341 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     2401 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     2461 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     2521 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     2581 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     2641 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     2701 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     2761 aggtttaaga atagtttttg ctgtactttc tgtagtgaat agagttaggc agggatattc
     2821 accattatcg tttcagacct ggagatctcc cgaggggacc cgacaggccc gaaggaatag
     2881 aagaagaagg tggagagaga gacagagaca gatccattcg accaattcac tcctcaggtg
     2941 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     3001 tgagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3061 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3121 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     3241 gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
     3301 ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
     3361 aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
     3421 tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
     3481 cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
     3541 gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
     3601 aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
     3661 gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
     3721 ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
     3781 caccccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4141 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4441 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5701 gagcgaggaa gcggaagagc gcccaatacg caaacc
//
