LOCUS       sequence-269938-        6100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing 
            variants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Lago-Docampo M, Tenorio J, Hernandez-Gonzalez I, Perez-Olivares C, 
            Escribano-Subias P, Pousada G, Baloira A, Arenas M, Lapunzina P, 
            Valverde D
  TITLE     Characterization of rare ABCC8 variants identified in Spanish 
            pulmonary arterial hypertension patients.
  JOURNAL   Sci Rep. 2020 Sep 15;10(1):15135. doi: 10.1038/s41598-020-72089-1.
  PUBMED    32934261
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-15"; volume: "10(1):15135. doi"; pages: " 
            10.1038/s41598-020-72089-1"
COMMENT     Sequence Label: pSPL3-ABCC8-9-10
FEATURES             Location/Qualifiers
     source          1..6100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        60..389
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      240..375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2605..2838
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3023..3067
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3382..3516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3406..3425)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3460..3479
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4190..4294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4295..4363,4364..5155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4513..4532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5326..5914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5815..5834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6068..6085
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      181 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      241 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      301 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
      361 gcttttttgg aggcctaggc ttttgcaaaa agcttggact gtgtttactt gcaatccccc
      421 aaaacagaca gaatggtgca tctgtccagt gaggagaagt ctgcggtcac tgccctgtgg
      481 ggcaaggtga atgtggaaga agttggtggt gaggccctgg gcaggctgct ggttgtctac
      541 ccatggaccc agaggttctt cgagtccttt ggggacctgt cctctgcaaa tgctgttatg
      601 aacaatccta aggtgaaggc tcatggcaag aaggtgctgg ctgccttcag tgagggtctg
      661 agtcacctgg acaacctcaa aggcaccttt gctaagctga gtgaactgca ctgtgacaag
      721 ctgcacgctc tagagtcgac ccagcagtaa gtaatacatg taatgcaacc tatacaaata
      781 gcaatagtag cattagtagt agcaataata atagcaatag ttgtgtggtc catagtaatc
      841 atagaatata ggaaaatatt aagacaaaga aaaatagaca ggttaattga tagactaata
      901 gaaagagcag aagacagtgg caatgagagt gaaggagaaa tatcagcact tgtggagatg
      961 ggggtggaga tggggcacca tgctccttgg gatgttgatg atctgtagtg ctacagaaaa
     1021 attgtgggtc acagtctatt atggggtagg gatcaccaga attcccctct gcttgcatga
     1081 gagcccatta tgctaacctc cttttgaaat tgctttgttt ctcctccttc cctgcactcc
     1141 actccccaag ataatttgga aacctgggca ccaaggcttg tcccactcta ggggactggg
     1201 gaaacaggga ttttgccagc acctcctggc agggcaacca tcaggtcaaa gggacctgac
     1261 ccatgacccc ttcctcagat cattgtgggt gtgattctcc tctactacat actcggagtc
     1321 agtgccttaa ttggagcagc tgtcatcatt ctactggctc ctgtccagta cttcgtggcc
     1381 accaagctgt ctcaggccca gcggagcaca ctggtgagtg cccaaaggtg tgtgtgtttt
     1441 gtccctcttc ccctccttcc ctcgacagca gcaggtctcc tcccacacac ccacactgtc
     1501 attgactttg actttgactt tgagtaggcc acttcacctg tccaggcctc agcttctttg
     1561 tctgggaaat ggagtcaatg ggggaggagg ctgcctcccg gtggggacag gaggcgggca
     1621 tggcagtgac agtattgaca ctcaggcctt cctctcgggt gggagacgga gcagaagcct
     1681 ctcccctctg actcggggac ccctgcagga gtattccaat gagcggctga agcagaccaa
     1741 cgagatgctc cgcggcatca agctgctgaa gctgtacgcc tgggagaaca tcttccgcac
     1801 gcgggtggag acgacccgca ggaaggagat gaccagcctc agggcctttg ccatctatac
     1861 ctccatctcc agtgagttca ccccacacag ccagccctgg gtgctgctgc gtacatgcaa
     1921 ggagagggtg tcagagctcg ggtaagaggg ggtgcaggga cagaagcagg aggccttgag
     1981 attatccgac tcagtagggg agcctcaaac tggctctgat agctaagctc ccagaaatca
     2041 aagtactctt cattcattta tccaacaaac atgcatggaa agcctgctat gtgctgctag
     2101 caaattaaga gaacaatttg gaaataataa aacaataatc tttaagcaat cctcaggagg
     2161 ggacccagaa attgtaacgc acagttttaa ttgtggaggg gaatttttct actgtaattc
     2221 aacacaactg tttaatagta cttggtttaa tagtacttgg agtactgaag ggtcaaataa
     2281 cactgaagga agtgacacaa tcacactccc atgcagaata aaacaattta taaacatgtg
     2341 gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagc ggacaaatta gatgttcatc
     2401 aaatattaca gggctgctat taacaagaga tggtggtaat aacaacaatg ggtccgagat
     2461 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     2521 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     2581 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     2641 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     2701 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     2761 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     2821 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     2881 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2941 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     3001 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     3061 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3121 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttctg tagtgaatag
     3181 agttaggcag ggatattcac cattatcgtt tcagacctgg agatctcccg aggggacccg
     3241 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgac
     3301 caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     3361 ctggctcaca aataccactg agatccagac atgataagat acattgatga gtttggacaa
     3421 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3481 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3541 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3601 tgtggtatgg ctgattatga tccccaggaa gctcctctgt gtcctcataa accctaacct
     3661 cctctacttg agaggacatt ccaatcatag gctgcccatc caccctctgt gtcctcctgt
     3721 taattaggtc acttaacaaa aaggaaattg ggtaggggtt tttcacagac cgctttctaa
     3781 gggtaatttt aaaatatctg ggaagtccct tccactgctg tgttccagaa gtgttggtaa
     3841 acagcccaca aatgtcaaca gcagaaacat acaagctgtc agctttgcac aagggcccaa
     3901 caccctgctc atcaagaagc actgtggttg ctgtgttagt aatgtgcaaa acaggaggca
     3961 cattttcccc acctgtgtag gttccaaaat atctagtgtt ttcattttta cttggatcag
     4021 gaacccagca ctccactgga taagcattat ccttatccaa aacagccttg tggtcagtgt
     4081 tcatctgctg actgtcaact gtagcatttt ttggggttac agtttgagca ggatatttgg
     4141 tcctgtagtt tgctaacaca ccccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4261 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4321 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4381 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4441 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4501 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4561 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4621 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4681 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4741 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4801 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4861 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4921 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4981 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5041 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5101 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5161 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5221 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5461 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5941 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6001 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6061 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
//
