LOCUS       sequence-269962-        7106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of 
            Renilla luciferase in the psiCHECK3 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: psiCHECK3-ZC3H15-Reverse-Complement
FEATURES             Location/Qualifiers
     source          1..7106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        114..471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        114..305
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(137..157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      322..457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          541..673
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        718..736
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      740..749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             746..1681
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2348..2396
                     /note="synthetic polyadenylation signal"
     promoter        2405..3156
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          3226..3358
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        3403..3421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3430..3439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3436..5088
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             4395..4403
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5113..5234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5124..5143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5178..5197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5367..5471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5472..5540,5541..6332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5690..5709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6503..7091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6992..7011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatctgc
       61 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
      121 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
      181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat
      481 tcttctgaca caacagtctc gaacttaagc tgcagaagtt ggtcgtgagg cactgggcag
      541 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
      601 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
      661 tttctctcca caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa
      721 tacgactcac tataggctag ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa
      781 acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg tgctggactc
      841 cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt ttctgcatgg
      901 taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag
      961 atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga atggctcata
     1021 tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga accttccaaa
     1081 gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact actcctacga
     1141 gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc
     1201 ctgggacgag tggcctgaca tcgaggagga tatcgccctg atcaagagcg aagagggcga
     1261 gaaaatggtg cttgagaata acttcttcgt cgagaccatg ctcccaagca agatcatgcg
     1321 gaaactggag cctgaggagt tcgctgccta cctggagcca ttcaaggaga agggcgaggt
     1381 tagacggcct accctctcct ggcctcgcga gatccctctc gttaagggag gcaagcccga
     1441 cgtcgtccag attgtccgca actacaacgc ctaccttcgg gccagcgacg atctgcctaa
     1501 gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg gagctaagaa
     1561 gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg aggacgctcc
     1621 agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga acgagcagta
     1681 attctaggcg atcgctcgag ttggatttta atgtatgatg gtctttattt acaactttat
     1741 tggcaaaaaa gaaaagggaa acttttaaac aatttaacac agggcattgt agctgatcct
     1801 gtcagataaa gaagttccat tttaaatgtc catctaattg tccaaagata cacaatactg
     1861 aatctgcata cgcagtttcc tttatgaagt acagtgctca tgttttaggc agtcttctaa
     1921 acatacaaat acagaggaaa ttaaatcact cattaaaaac tgtgtcaaat gaagggtatt
     1981 ttaaatataa gtttgttgtt tctggtaata gcacatgccc aaatgaaaac caaaagatgg
     2041 aaaggcagat acacttctta cttgggtgga cagttacaac atcaatcatt ttctgcaatg
     2101 accattatat tttcaattta tcatgaacta ctctgaactt actatgagat gtagccaaag
     2161 tcagaagaga agatgtaggg acaagatgcc ttttttgcag gaggttaact cctccagaat
     2221 cagcatagga aataaacatc ctgttgattc taggtggaaa gaaattaatg tagtcaattt
     2281 caactcgtgc tgagctgact taattttttc agcgatgtgt ttggtaccta gagcggccgc
     2341 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgagga
     2401 tctaaatgag tcttcggacc tcgcgggggc cgcttaagcg gtggttaggg tttgtctgac
     2461 gcggggggag ggggaaggaa cgaaacactc tcattcggag gcggctcggg gtttggtctt
     2521 ggtggccacg ggcacgcaga agagcgccgc gatcctctta agcacccccc cgccctccgt
     2581 ggaggcgggg gtttggtcgg cgggtggtaa ctggcgggcc gctgactcgg gcgggtcgcg
     2641 cgccccagag tgtgaccttt tcggtctgct cgcagacccc cgggcggcgc cgccgcggcg
     2701 gcgacgggct cgctgggtcc taggctccat ggggaccgta tacgtggaca ggctctggag
     2761 catccgcacg actgcggtga tattaccgga gaccttctgc gggacgagcc gggtcacgcg
     2821 gctgacgcgg agcgtccgtt gggcgacaaa caccaggacg gggcacaggt acactatctt
     2881 gtcacccgga ggcgcgaggg actgcaggag cttcagggag tggcgcagct gcttcatccc
     2941 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     3001 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     3061 gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc
     3121 ctcgaacacc gagcgaccct gcagcgaccc gcttaaaagc ttgattcttc tgacacaaca
     3181 gtctcgaact taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
     3241 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     3301 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     3361 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
     3421 gctagcaaag ccaccatggc cgatgctaag aacattaaga agggccctgc tcccttctac
     3481 cctctggagg atggcaccgc tggcgagcag ctgcacaagg ccatgaagag gtatgccctg
     3541 gtgcctggca ccattgcctt caccgatgcc cacattgagg tggacatcac ctatgccgag
     3601 tacttcgaga tgtctgtgcg cctggccgag gccatgaaga ggtacggcct gaacaccaac
     3661 caccgcatcg tggtgtgctc tgagaactct ctgcagttct tcatgccagt gctgggcgcc
     3721 ctgttcatcg gagtggccgt ggcccctgct aacgacattt acaacgagcg cgagctgctg
     3781 aacagcatgg gcatttctca gcctaccgtg gtgttcgtgt ctaagaaggg cctgcagaag
     3841 atcctgaacg tgcagaagaa gctgcctatc atccagaaga tcatcatcat ggactctaag
     3901 accgactacc agggcttcca gagcatgtac acattcgtga catctcatct gcctcctggc
     3961 ttcaacgagt acgacttcgt gccagagtct ttcgacaggg acaaaaccat tgccctgatc
     4021 atgaacagct ctgggtctac cggcctgcct aagggcgtgg ccctgcctca tcgcaccgcc
     4081 tgtgtgcgct tctctcacgc ccgcgaccct attttcggca accagatcat ccccgacacc
     4141 gctattctga gcgtggtgcc attccaccac ggcttcggca tgttcaccac cctgggctac
     4201 ctgatttgcg gctttcgggt ggtgctgatg taccgcttcg aggaggagct gttcctgcgc
     4261 agcctgcaag actacaaaat tcagtctgcc ctgctggtgc caaccctgtt cagcttcttc
     4321 gctaagagca ccctgatcga caagtacgac ctgtctaacc tgcacgagat tgcctctggc
     4381 ggcgccccac tgtctaagga ggtgggcgaa gccgtggcca agcgctttca tctgccaggc
     4441 atccgccagg gctacggcct gaccgagaca accagcgcca ttctgattac cccagagggc
     4501 gacgacaagc ctggcgccgt gggcaaggtg gtgccattct tcgaggccaa ggtggtggac
     4561 ctggacaccg gcaagaccct gggagtgaac cagcgcggcg agctgtgtgt gcgcggccct
     4621 atgattatgt ccggctacgt gaataaccct gaggccacaa acgccctgat cgacaaggac
     4681 ggctggctgc actctggcga cattgcctac tgggacgagg acgagcactt cttcatcgtg
     4741 gaccgcctga agtctctgat caagtacaag ggctaccagg tggccccagc cgagctggag
     4801 tctatcctgc tgcagcaccc taacattttc gacgccggag tggccggcct gcccgacgac
     4861 gatgccggcg agctgcctgc cgccgtcgtc gtgctggaac acggcaagac catgaccgag
     4921 aaggagatcg tggactatgt ggccagccag gtgacaaccg ccaagaagct gcgcggcgga
     4981 gtggtgttcg tggacgaggt gcccaagggc ctgaccggca agctggacgc ccgcaagatc
     5041 cgcgagatcc tgatcaaggc taagaaaggc ggcaagatcg ccgtgtaatg cggccgcttc
     5101 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5281 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     5341 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5401 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5461 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5521 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5581 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5641 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5701 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5761 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5821 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5881 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5941 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6001 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6061 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6121 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6181 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6241 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6301 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6361 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6421 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6541 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6601 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6661 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6721 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6781 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6841 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6901 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6961 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7021 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7081 gcctatggaa aaacgccagc aacgcg
//
