LOCUS       sequence-269998-        9570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Clustered Reporter. Fluorescent reporter to be used with m6A readers
            and writers. (5xZF binding sites + 63xGATC 
            sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Park M, Patel N, Keung AJ, Khalil AS
  TITLE     Engineering Epigenetic Regulation Using Synthetic Read-Write 
            Modules.
  JOURNAL   Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 
            10.1016/j.cell.2018.11.002. Epub 2018 Dec 6.
  PUBMED    30528434
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2019-01-10"; volume: "176(1-2):227-238.e20. doi"; pages: " 
            10.1016/j.cell.2018.11.002. Epub 2018 Dec 6"
COMMENT     Sequence Label: pMP498
FEATURES             Location/Qualifiers
     source          1..9570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(88..879,880..948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     711..730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(949..1053)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1207..1736
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     3675..3699
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3719..3728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3725..3727,3728..3730,3731..4441,4442..4447,
                     4448..4570)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(3770..3791)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4031..4050)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4378..4399
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4656..4675)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4721..4776
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4775..4794)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        5143..5642
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5178..5201)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5575..5594
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5675..6271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5675..5694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6171..6191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6287..6340
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6347..7054
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6489..6507)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6757..6776
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7064..7288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    7487..7988
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(8173..8189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8173..8189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8186..8208)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8197..8213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8221..8227,8228..8245,8246..8251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8266..8287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      8372..8506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8526..8660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8563..8582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8617..8636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8728..8745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8899..9487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8979..8998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
       61 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      121 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
      181 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
      241 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
      301 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
      361 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
      421 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
      481 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
      541 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
      601 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
      661 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
      721 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
      781 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
      841 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
      901 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
      961 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1021 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
     1081 gtcgacattg attattgact agtgaagggg caggagagcc aggggcatga gatggtggac
     1141 gaggaagggg gacagggaag cctgagcgcc tctcctgggc ttgccaagga ctcaaaccca
     1201 gaagcccaga gcagggcctt agggaagcgg gaccctgctc tgggcggagg aatatgtccc
     1261 agatagcact ggggactctt taaggaaaga aggatggaga aagagaaagg gagtagaggc
     1321 ggccacgacc tggtgaacac ctaggacgca ccattctcac aaagggagtt ttccacacgg
     1381 acacccccct cctcaccaca gccctgccag gacggggctg gctactggcc ttatctcaca
     1441 ggtaaaactg acgcacggag gaacaatata aattggggac tagaaaggtg aagagccaaa
     1501 gttagaactc aggaccaact tattctgatt ttgtttttcc aaactgcttc tcctcttggg
     1561 aagtgtaagg aagctgcagc accaggatca gtgaaacgca ccagacagcc gcgtcagagc
     1621 agctcaggtt ctgggagagg gtagcgcagg gtggccactg agaaccgggc aggtcacgca
     1681 tccccccctt ccctcccacc ccctgccaag ctctccctcc caggatcctc tctggctcca
     1741 tcgtaagcaa accttagagg ttctggcaag gagagagatg gctccaggaa atgggggtgt
     1801 gtcaccagat aaggaatctg cctaacagga ggtgggggtt agacccaata tcaggagact
     1861 aggaaggagg aggcctaagg atggggcttt tctgtcacca atcaggcttg accgacaatt
     1921 ggaaaagtgc cacctgggtc gaccggcgta gccgatgtcg cgcaaccacc agtaagtatc
     1981 tcacggcgta gccgatgtcg cgccagtaaa ggcttctcta caccggcgta gccgatgtcg
     2041 cgcctccttc gggataaaat ctccggcgta gccgatgtcg cgcagtgtgg tagactatct
     2101 ctgcggcgta gccgatgtcg cgctaaaggc ttactgagca ctagatcgaa gttgtctctg
     2161 aatcacagat cgttacgata ctttacacgg tgatcgtggt attgttgtga cactagatca
     2221 gatagggtgt gattggttag atcaatcgtt gcgtaatcta caagatctat ttctccaact
     2281 ttcgcaagat cctttctgag taagtagcac cgatctctgg aggataatct cagtcgatcg
     2341 ataataaacc tggctcctgg atcgattact gaggatacgc ttcgatcgtt tccaactact
     2401 ggctttagat cgagattcgt aaaaggctga tgatcgaact ttctctgcgt ctattgatcc
     2461 ctgaagacct attgcttacg atcaataata ctatcgggcg agagatcagt aggtgaggat
     2521 tacacaagat ccgaatctct ggcttcaata agatctgtgg ttattactca gggatgatcg
     2581 gcgaaacaat cttctctatg atccgattgc gagatactat tgtgatccag tgtgccaata
     2641 agttttcgat caaataatac tgtgccagag cgatcaggat aacaccctgt gaatagatca
     2701 tcagacagtc ggattctatg atcgaaagtc tgagcgattt actgatcagc gacaactatt
     2761 tactgacgat catcaacgat agaggtgaga tgatctaaac cgatacgggt aactagatcc
     2821 cttctgtatt tctactggcg atcatccacc aatacctgag ttagatcgaa tcagccttta
     2881 cagtagggat ccagtcctac tatcaccaga tgatcgctct cagtttctat ttcgtgatca
     2941 cagtagtgag aaccagttag atcataagtg ttgatacctc ccagatcctc tgaaataggc
     3001 agttaccgat ccagggctta caatagaact cgatctacac cacaatagaa gggatgatcg
     3061 atactattcc gaggattgcg atccttattg ctaccaggaa ctcgatcctg gaataagaag
     3121 gtgctacgat ccgagaatca cgatagtaag ggatcagcac gattctcctt ttatcgatca
     3181 actttcctat taccaacggg atctgaagaa tagatttcct gcggatctat tttccgtcgt
     3241 aactcaggat cgaataagaa tcgctctggt tgatcgcttt atcactgggt aagaggatca
     3301 gttgaatact gtgggtagag atcgagtaaa acccgttcac ttagatcctc tcaataaccg
     3361 atagacggat ctttattcaa ccttctgcga agatcgcttt taccagtcct gataggatcg
     3421 aaacagaacc gaaaatcagg atcggaggct attgctactc tatgatctga tactcaatcg
     3481 tcaggtagat caaatagtcc gtaagtaggc tgatcccagt tgaatagtaa gagccgatct
     3541 actcaactgg tgggataatg atcttttatt gagttcagcc gaagatcgga taaacttcta
     3601 ccagcaggat ctatccgtga ttatctggac agatcactag ttaggcgtgt acggtgggag
     3661 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggaacgc gtaccggtgc
     3721 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3781 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3841 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3901 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3961 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4021 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4081 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4141 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4201 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4261 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4321 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4381 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4441 gaagcttagc catggcttcc cgccggaggt ggaggagcag gatgatggca cgctgcccat
     4501 gtcttgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctt ctgctaggat
     4561 caatgtgtag gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     4621 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     4681 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     4741 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     4801 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     4861 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     4921 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4981 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     5041 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     5101 tccctcgacc tgcagcccaa gcttggcgta atgaattcta ccgggtaggg gaggcgcttt
     5161 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     5221 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     5281 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     5341 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     5401 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     5461 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     5521 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     5581 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     5641 cgacctgcag cccaagcttc ctaggaccga tatcatgacc gagtacaagc ccacggtgcg
     5701 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5761 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     5821 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     5881 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     5941 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6001 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6061 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6241 cacctggtgc atgacccgca agcccggtgc cggtaccgga agcggagagg gcagaggaag
     6301 tctgctaaca tgcggtgacg tcgaggagaa tcctggacct gcatgcgtga gcaagggcga
     6361 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     6421 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6481 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     6541 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     6601 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6661 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6721 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     6781 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     6841 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     6901 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     6961 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     7021 ctccaccggc ggcatggacg agctgtacaa gtaagtttaa accctgtgcc ttctagttgc
     7081 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7141 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     7201 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     7261 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7321 agggggtatc cccacgcgcc ctgtagcgct cgagctgtcc ctagtggccc cactgtgggg
     7381 tggaggggac agataaaagt acccagaacc agagccacat taaccggccc tgggaatata
     7441 aggtggtccc agctcgggga cacaggatcc ctggaggcag caaacatgct gtcctgaagt
     7501 ggacataggg gcccgggttg gaggaagaag actagctgag ctctcggacc cctggaagat
     7561 gccatgacag ggggctggaa gagctagcac agactagaga ggtaaggggg gtaggggagc
     7621 tgcccaaatg aaaggagtga gaggtgaccc gaatccacag gagaacgggg tgtccaggca
     7681 aagaaagcaa gaggatggag aggtggctaa agccagggag acggggtact ttggggttgt
     7741 ccagaaaaac ggtgatgatg caggcctaca agaaggggag gcgggacgca agggagacat
     7801 ccgtcggaga aggccatcct aagaaacgag agatggcaca ggccccagaa ggagaaggaa
     7861 aagggaaccc agcgagtgaa gacggcatgg ggttgggtga gggaggagag atgcccggag
     7921 aggacccaga cacggggagg atccgctcag aggacatcac gtggtgcagc gccgagaagg
     7981 aagtgctccg gaaagagcat ccttgggcag caacacagca gagagcaagg ggaagaggga
     8041 gtggaggaag acggaacctg aaggaggcgg cagggaagga tctgggccag ccgtagaggt
     8101 gacccaggcc acaagctgca gacagaaagc ggcacaggcc caggggagag aatgaagctt
     8161 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8221 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8281 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     8341 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc cagttccgcc
     8401 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8461 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8521 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8641 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8701 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8761 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8821 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8881 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8941 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9001 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9061 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9121 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9181 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9241 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9301 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9361 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9421 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9481 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9541 acgttaaggg attttggtca tgagattatc
//
