LOCUS       sequence-27006-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control Lentiviral vector with strong EF1a promoter, expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, 
            Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
  TITLE     Isolation of human iPS cells using EOS lentiviral vectors to select 
            for pluripotency.
  JOURNAL   Nat Methods. 2009 May . 6(5):370-6.
  PUBMED    19404254
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 May . 6(5):370-6."
COMMENT     Sequence Label: PL-SIN-EF1-alpha-EGFP
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          50..253
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(73..93)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cnngngacgc tcctcgccct tgctcaccat ggctaattcc tcacgacacc tgaaatggaa
       61 gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa
      121 gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc
      181 actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac
      241 tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac
      301 atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc
      361 gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc
      421 atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga
      481 tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc
      541 cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac
      601 gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg
      661 catttacaag actatcttgc cagaaaaaaa nncgtcgcag cangtcatca aaaattttaa
      721 atggctagag acttatcgaa agcagcgaga caggcgcgaa ggtgccacca gattcacacg
      781 cngnngnccc annncccang ccaagnnctc cac
//
