LOCUS       sequence-270060-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-154-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-154-5p
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(102..126)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(350..369)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    415..470
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(469..488)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ggccgacaaa gagacatacg tcgagcagca cgaggtggct gtggccagat actgcgacct
       61 ccctagcaaa ctggggcaca aacttaattg agaattctac cacccagctt tcttgtacaa
      121 agtggtacgt ctcaagacca gaggtctctg cttttgaata tcgaaggcaa cacggataac
      181 ctacgaaggc aacacggata acctacgaag gcaacacgga taacctacga aggcaacacg
      241 gataacctac aaccagaaga gacctgtgct tcactcctca ggtgcaggct gcctatcaga
      301 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
      361 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
      421 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
      481 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
      541 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
      601 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
      661 tttttttt
//
