LOCUS       sequence-270077-       10145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. CTLA4)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF197
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1211..1220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2396..2416
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2450..2467
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2474..3184
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2619..2637)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2887..2906
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3236..3253)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3242..3466
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3828..4131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4132..4335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4286..4306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4332..4356
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4473..4569
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     4626..4645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4626..4644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4655..5677
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    5694..5828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5718..5737)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5772..5791
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5981..6085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6086..6154,6155..6946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6304..6323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7117..7705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7606..7625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7859..7876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8250..8269
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    8268..8323
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8369..8388
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(8488..9513)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8488..9513)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        join(10145,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccatactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt aaggtgtcgt gaaaactacc ccaagctggc
     1201 ctctgcggcc gccaccatgg ttctcctgag atccaggaga caaatgggaa agcttcacca
     1261 tcccaagaac tggccctgca cagcaatgct ctctcttctc tttattccaa gtatctctaa
     1321 aggggtacat gtgactcaac cagaagtggt cttggccaac agcagagggg ttgccagctt
     1381 tgtgtgtaaa tatgaattga caaacaaaac caaagaggtc cgagtggggc ttcttcgaca
     1441 gatggacaac cagatggttg aagtctgtgc ctcaacatat ctggtacagg atcaaccagt
     1501 gttcatggat gacatgcttg agtgtacggg acatgtcaat gggagtaaag tgacgctcac
     1561 tctcaccggg ctaaaggcct cagacagtgg actatacatc tgcaaagtgg aactcatgta
     1621 tcctccaccc tactacatgg gtttgggcaa tggaacacaa atatatgtaa ttgatgctga
     1681 accttgtcca gactttgaag atatcccaaa gcccacagaa tgtacatgct gcaaatgctc
     1741 agcatgtgat gttggtggac tttctgtatt catcttcccc ccaaatccta aggataccct
     1801 caaactctca ggatcaccta aaatcacctg tatggtggtt gatgtgagtg atgcatcaga
     1861 tgttaaaatt gcctggttca aaaataataa ggaaattgac agtcctaaac caacacagaa
     1921 ggaacaaaac aatgggacca ttcaggtgat cagcactctc tctgtagtcc accaagaatg
     1981 gctgaaaggc acagtgtata cctgtaaagt tgataccaaa gaattaccat ttcctgaaac
     2041 aaggaccatc tcccacactt caggtgacag aaagatgcct gatatgtatg tctttgcccc
     2101 acatcctgat gagttgaagc ataaggatac tgttagtctt acctgcttcg taaaaaattt
     2161 cttccctcaa gacattgttg ttgaatggca atgcaatgac catccagagc ctgaagaaaa
     2221 ctattatacc accccagcaa tgaaggaaaa ggacaccttc tttgtctaca gcaaactcac
     2281 tctgaataaa gcaaaatggc aagagaataa ctcctatacc tgcatggtac tgcatgaggg
     2341 ctttccaaac caatattccc agaggacaat ctctgctagc ccaggtaaac ccggggagaa
     2401 cctttacttc cagggatccg ctggctccgc tgctggttct ggcgaattcc atcatcacca
     2461 tcaccatgtc gacatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     2521 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     2581 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     2641 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     2701 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     2761 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     2821 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     2881 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     2941 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3001 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3061 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3121 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3181 gtaggctagc gtcaggccaa gcttggggat caattctcta gagctcgctg atcagcctcg
     3241 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3301 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3361 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3421 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3481 agaaccagct gggcctacta gatagcggac cccttaccga aacatcgccg cattctgcag
     3541 aggagtcgaa cttagtgtat gtaaacttct gacccactgg aattgtgata cagtgaatta
     3601 taagtgaaat aatctgtctg taaacaattg ttggaaaaat gacttgtgtc atgcacaaag
     3661 tagatgtcct aactgacttg ccaaaactat tgtttgttaa caagaaattt gtggagtagt
     3721 tgaaaaacga gttttaatga ctccaactta agtgtatgta aacttccgac ttcaactgta
     3781 tagggatcca attttaacaa aatattcgag cttgcatgcc tgcaggtcgt tacataactt
     3841 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3901 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3961 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     4021 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     4081 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     4141 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4201 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4261 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4321 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     4381 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctaga ggatccggta
     4441 ctcgaggaac tgaaaaacca gaaagttaac tggtaagttt agtctttttg tcttttattt
     4501 caggtcccgg atccggtggt ggtgcaaatc aaagaactgc tcctcagtgg atgttgcctt
     4561 tacttctagg cctgtacgga agtgttactt ctgctctaaa agctgcggaa ttgtacccgc
     4621 ggaattaata cgactcacta tagggactag taccatggga aaatcaaaag aaatcagcca
     4681 agacctcaga aaaagaattg tagacctcca caagtctggt tcatccttgg gagcaatttc
     4741 caaacgcctg gcggtaccac gttcatctgt acaaacaata gtacgcaagt ataaacacca
     4801 tgggaccacg cagccgtcat accgctcagg aaggagacgc gttctgtctc ctagagatga
     4861 acgtactttg gtgcgaaaag tgcaaatcaa tcccagaaca acagcaaagg accttgtgaa
     4921 gatgctggag gaaacaggta caaaagtatc tatatccaca gtaaaacgag tcctatatcg
     4981 acataacctg aaaggccact cagcaaggaa gaagccactg ctccaaaacc gacataagaa
     5041 agccagacta cggtttgcaa ctgcacatgg ggacaaagat cgtacttttt ggagaaatgt
     5101 cctctggtct gatgaaacaa aaatagaact gtttggccat aatgaccatc gttatgtttg
     5161 gaggaagaag ggggaggctt gcaagccgaa gaacaccatc ccaaccgtga agcacggggg
     5221 tggcagcatc atgttgtggg ggtgctttgc tgcaggaggg actggtgcac ttcacaaaat
     5281 agatggcatc atggacgcgg tgcagtatgt ggatatattg aagcaacatc tcaagacatc
     5341 agtcaggaag ttaaagcttg gtcgcaaatg ggtcttccaa cacgacaatg accccaagca
     5401 tacttccaaa gttgtggcaa aatggcttaa ggacaacaaa gtcaaggtat tggagtggcc
     5461 atcacaaagc cctgacctca atcctataga aaatttgtgg gcagaactga aaaagcgtgt
     5521 gcgagcaagg aggcctacaa acctgactca gttacaccag ctctgtcagg aggaatgggc
     5581 caaaattcac ccaaattatt gtgggaagct tgtggaaggc tacccgaaac gtttgaccca
     5641 agttaaacaa tttaaaggca atgctaccaa atactagggg ccctaaccgc ggggatccag
     5701 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5761 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5821 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     5881 aggtttttta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     5941 atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7261 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7741 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7801 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7861 cgagtcagtg agcgaggaag cggatcccta tacagttgaa gtcggaagtt tacatacacc
     7921 ttagccaaat acatttaaac tcactttttc acaattcctg acatttaatc ctagtaaaaa
     7981 ttccctgtct taggtcagtt aggatcacca ctttatttta agaatgtgaa atatcagaat
     8041 aatagtagag agaatgattc atttcagctt ttatttcttt catcacattc ccagtgggtc
     8101 agaagtttac atacactcga ctcctctgca gaatgcggcg atgtttcggt aaggggtccc
     8161 gagtagctag ttcatggcag ccagcatatg gcatatgttg ccaaactcta aaccaaatac
     8221 tcattctgat gttttaaatg atttgccctc ccatatgtcc ttccgagtga gagacacaaa
     8281 aaattccaac acactattgc aatgaaaata aatttccttt attagccaga agtcagatgc
     8341 tcaaggggct tcatgatgtc cccataattt ttggcagagg gaaaaagatc tcagtggtat
     8401 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
     8461 ggagtgaatt cacgacaggc cttcgaacta ttcctttgcc ctcggacgag tgctggggcg
     8521 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
     8581 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
     8641 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
     8701 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
     8761 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
     8821 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
     8881 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
     8941 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     9001 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     9061 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     9121 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     9181 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
     9241 gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg gaatcgggag
     9301 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
     9361 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
     9421 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
     9481 gacagacgtc gcggtgagtt caggcttttt catggtggcc tcaggtgcag aggtttctac
     9541 agggaaagag agaaaggcta cagttagttt gctgtgatga tgctaagtgc cgaggtcaga
     9601 tgtctatgga ttgtacacgg aatcccaacg caggaaccaa aaaacactca cccaaaaaaa
     9661 tggcgctaag gccgagagaa aggcgaagtt ccgtctacgg ctatatacct tgaaacaggg
     9721 gcggcagaag tgacgcaacg cggttgcgcg gacggcttcc tgtgttttca ttggtcgaag
     9781 cgttctgcgc gtgcgcagag attccccagg gaaggcgggc cctcttccga atgtccaatc
     9841 cggacattct tccggttgga cctccgcggt cagcgctgtg cccggcgaga gatcacgtgg
     9901 ggcgcggagg cggtgctgct ggggcacggc cgtccagcct cggcggccat atttttgagg
     9961 ggctgttcat ctcgttcaca cgctctgtcc gccatgtttg tgagtggaag cgccattacc
    10021 ccttcaagcg actgaaggct gcagggcctc tggtggcccg catggggaga ccagacccgc
    10081 caggcccgcc tttccgcact cagtccgggc ttactttatt ttgtgagaca gggtctcgcc
    10141 tagag
//
