LOCUS       sequence-270078-       10078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for stable transfection of 
            eukaryotic cells with luciferse and hygromycin restistance. CMV 
            protomor drives SB100X transposase for transposon integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF112
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        552..1734
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     1682..1702
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1760..1769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2009..2026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2015..2239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2601..2904
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2905..3108
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3059..3079
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3105..3129
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3246..3342
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     3399..3418
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3399..3417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3428..4450
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    4467..4601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4491..4510)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4545..4564
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4754..4858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4859..4927,4928..5719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5077..5096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5890..6478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6379..6398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6632..6649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7023..7042
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7041..7096
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7142..7161
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7261..8286)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7261..8286)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8287..8343)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8353..10002)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      9999..10008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtcagatgt ctatggattg tacacggaat cccaacgcag gaaccaaaaa acactcaccc
       61 aaaaaaatgg cgctaaggcc gagagaaagg cgaagttccg tctacggcta tataccttga
      121 aacaggggcg gcagaagtga cgcaacgcgg ttgcgcggac ggcttcctgt gttttcattg
      181 gtcgaagcgt tctgcgcgtg cgcagagatt ccccagggaa ggcgggccct cttccgaatg
      241 tccaatccgg acattcttcc ggttggacct ccgcggtcag cgctgtgccc ggcgagagat
      301 cacgtggggc gcggaggcgg tgctgctggg gcacggccgt ccagcctcgg cggccatatt
      361 tttgaggggc tgttcatctc gttcacacgc tctgtccgcc atgtttgtga gtggaagcgc
      421 cattacccct tcaagcgact gaaggctgca gggcctctgg tggcccgcat ggggagacca
      481 gacccgccag gcccgccttt ccgcactcag tccgggctta ctttattttg tgagacaggg
      541 tctcgcctag aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
      601 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
      661 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      721 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      781 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
      841 tgccttgaat tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg
      901 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      961 tgagttgagg cctggcttgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     1021 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     1081 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     1141 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1201 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     1261 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     1321 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     1381 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1441 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1501 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1561 gggttttatg cgatggagtt tccccatact gagtgggtgg agactgaagt taggccagct
     1621 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1681 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttaaggtgtc gtgaaaacta
     1741 ccccaagctg gcctctgagg ccaccatggc tcgagtttcg aactcaatta tactgaattg
     1801 tttcatcata gggttcaaca ttgtgataaa tcaggacttc aatgtctagt gttcagtaaa
     1861 taccttgtag ggctctccaa aaaggggaat cttttcagga aatgcctctt tctcttggtg
     1921 ggcttcctgg ttgcgtcttt ccttctcact aattcgaagg cctgtcaggc caagcttggg
     1981 gatcaattct ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     2041 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2101 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2161 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2221 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctgggccta ctagatagcg
     2281 gaccccttac cgaaacatcg ccgcattctg cagaggagtc gaacttagtg tatgtaaact
     2341 tctgacccac tggaattgtg atacagtgaa ttataagtga aataatctgt ctgtaaacaa
     2401 ttgttggaaa aatgacttgt gtcatgcaca aagtagatgt cctaaccgac ttgccaaaac
     2461 tattgtttgt taacaagaaa tttgtggagt agttgaaaaa cgagttttaa tgactccaac
     2521 ttaagtgtat gtaaacttcc gacttcaact gtatagggat ccaattttaa caaaatattc
     2581 gagcttgcat gcctgcaggt cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2641 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2701 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2761 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2821 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2881 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2941 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3001 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3061 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3121 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     3181 cgatccagcc tccggactct agaggatccg gtactcgagg aactgaaaaa ccagaaagtt
     3241 aactggtaag tttagtcttt ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa
     3301 atcaaagaac tgctcctcag tggatgttgc ctttacttct aggcctgtac ggaagtgtta
     3361 cttctgctct aaaagctgcg gaattgtacc cgcggaatta atacgactca ctatagggac
     3421 tagtaccatg ggaaaatcaa aagaaatcag ccaagacctc agaaaaagaa ttgtagacct
     3481 ccacaagtct ggttcatcct tgggagcaat ttccaaacgc ctggcggtac cacgttcatc
     3541 tgtacaaaca atagtacgca agtataaaca ccatgggacc acgcagccgt cataccgctc
     3601 aggaaggaga cgcgttctgt ctcctagaga tgaacgtact ttggtgcgaa aagtgcaaat
     3661 caatcccaga acaacagcaa aggaccttgt gaagatgctg gaggaaacag gtacaaaagt
     3721 atctatatcc acagtaaaac gagtcctata tcgacataac ctgaaaggcc actcagcaag
     3781 gaagaagcca ctgctccaaa accgacataa gaaagccaga ctacggtttg caactgcaca
     3841 tggggacaaa gatcgtactt tttggagaaa tgtcctctgg tctgatgaaa caaaaataga
     3901 actgtttggc cataatgacc atcgttatgt ttggaggaag aagggggagg cttgcaagcc
     3961 gaagaacacc atcccaaccg tgaagcacgg gggtggcagc atcatgttgt gggggtgctt
     4021 tgctgcagga gggactggtg cacttcacaa aatagatggc atcatggacg cggtgcagta
     4081 tgtggatata ttgaagcaac atctcaagac atcagtcagg aagttaaagc ttggtcgcaa
     4141 atgggtcttc caacacgaca atgaccccaa gcatacttcc aaagttgtgg caaaatggct
     4201 taaggacaac aaagtcaagg tattggagtg gccatcacaa agccctgacc tcaatcctat
     4261 agaaaatttg tgggcagaac tgaaaaagcg tgtgcgagca aggaggccta caaacctgac
     4321 tcagttacac cagctctgtc aggaggaatg ggccaaaatt cacccaaatt attgtgggaa
     4381 gcttgtggaa ggctacccga aacgtttgac ccaagttaaa caatttaaag gcaatgctac
     4441 caaatactag gggccctaac cgcggggatc cagacatgat aagatacatt gatgagtttg
     4501 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4561 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4621 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaacgctta caatttcctg
     4681 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatcag gtggcacttt
     4741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6001 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggatcc
     6661 ctatacagtt gaagtcggaa gtttacatac accttagcca aatacattta aactcacttt
     6721 ttcacaattc ctgacattta atcctagtaa aaattccctg tcttaggtca gttaggatca
     6781 ccactttatt ttaagaatgt gaaatatcag aataatagta gagagaatga ttcatttcag
     6841 cttttatttc tttcatcaca ttcccagtgg gtcagaagtt tacatacact cgactcctct
     6901 gcagaatgcg gcgatgtttc ggtaaggggt cccgagtagc tagttcatgg cagccagcat
     6961 atggcatatg ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc
     7021 ctcccatatg tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa
     7081 ataaatttcc tttattagcc agaagtcaga tgctcaaggg gcttcatgat gtccccataa
     7141 tttttggcag agggaaaaag atctcagtgg tatttgtgag ccagggcatt ggccacacca
     7201 gccaccacct tctgataggc agcctgcacc tgaggagtga attcacgaca ggccttcgaa
     7261 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     7321 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     7381 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     7441 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     7501 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     7561 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     7621 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     7681 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     7741 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     7801 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     7861 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     7921 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     7981 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     8041 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     8101 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     8161 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     8221 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     8281 tttcatgggg ccaggattct cctcgacgtc accagcctgc ttcagcaggc tgaagttagt
     8341 agctccactg cccacggcga tcttgccgcc cttcttggcc ttaatgagaa tctcgcggat
     8401 cttgcgggcg tccaacttgc cggtcagtcc tttaggcacc tcgtccacga acacaacacc
     8461 accgcgcagc ttcttggcgg ttgtaacctg gctggccaca tagtccacga tctccttctc
     8521 ggtcatggtt ttaccgtgtt ccagcacgac gactgcggcg ggcagctcgc cggcatcgtc
     8581 gtcgggcagg ccggcgaccc cggcgtcgaa gatgttgggg tgttgcagca ggatgctctc
     8641 cagttcggct ggggctacct ggtagccctt gtatttgatc aggctcttca gccggtccac
     8701 gatgaagaag tgctcgtcct cgtcccagta ggcgatgtcg ccgctgtgca gccagccgtc
     8761 cttgtcgatg agagcgtttg tagcctcggg gttgttaacg tagccgctca tgatcatggg
     8821 gccacggacg cacagctcgc cgcgctggtt cacacccagt gtcttaccgg tgtccaagtc
     8881 caccacctta gcctcgaaga agggcaccac cttgcctact gcgccaggct tgtcgtcccc
     8941 ttcgggggtg atcagaatgg cgctggttgt ttctgtcagg ccgtagccct ggcggatgcc
     9001 tggtaggtgg aagcgtttgg ccacggcctc acctacctcc ttgctgagcg gcgccccgcc
     9061 gctggcgatc tcgtgcaagt tgcttaggtc gtacttgtcg atgagagtgc tcttagcgaa
     9121 gaagctaaat agtgtgggca ccagcagggc agattgaatc ttatagtctt gcaagctgcg
     9181 caagaatagc tcctcctcga agcggtacat gagcacgacc cgaaagccgc agatcaagta
     9241 gcccagcgtg gtgaacatgc cgaagccgtg gtgaaatggc accacgctga ggatagcggt
     9301 gtcggggatg atctggttgc cgaagatggg gtcgcgggca tgactgaatc ggacacaagc
     9361 ggtgcggtgc ggtagggcta cgcccttggg caatccggta ctgccactac tgttcatgat
     9421 cagggcgatg gttttgtccc ggtcgaagct ctcgggcacg aagtcgtact cgttgaagcc
     9481 gggtggcaaa tgggaagtca cgaaggtgta catgctttgg aagccctggt agtcggtctt
     9541 gctatccatg atgatgatct tttgtatgat cggtagcttc ttttgcacgt tgaggatctt
     9601 ttgcagccct ttcttgctca cgaatacgac ggtgggctgg ctgatgccca tgctgttcag
     9661 cagctcgcgc tcgttgtaga tgtcgttagc tggggccaca gccacaccga tgaacagggc
     9721 acccaacacg ggcatgaaga actgcaagct attctcgctg cacaccacga tccgatggtt
     9781 tgtattcagc ccatagcgct tcatagcttc tgccagccga acgctcatct cgaagtactc
     9841 ggcgtaggta atgtccacct cgatatgtgc gtcggtaaag gcgatggtgc cgggcaccag
     9901 ggcgtagcgc ttcatggctt tgtgcagctg ctcgccggcg gtcccgtctt cgagtgggta
     9961 gaatggcgct gggcccttct taatgttttt ggcatcttcc atggtggcgg tgcagaggtt
    10021 tctacaggga aagagagaaa ggctacagtt agtttgctgt gatgatgcta agtgccga
//
