LOCUS       sequence-270079-        9551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF161
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     956..976
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1037..1046
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1622..1642
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1676..1693
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(1700..1702,1703..2416)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKA
                     NFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1742..1763)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2003..2022)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2350..2371
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2468..2485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2474..2698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3060..3363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3364..3567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3518..3538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3564..3588
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3705..3801
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     3858..3877
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3858..3876
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3887..4909
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    4926..5060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4950..4969)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5004..5023
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5213..5317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5318..5386,5387..6178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5536..5555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6349..6937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6838..6857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7091..7108
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7482..7501
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7500..7555
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7601..7620
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7720..8745)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7720..8745)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        join(9377..9551,1..1008)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
       61 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      121 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      181 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      241 gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      301 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      361 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      421 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      481 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      541 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      601 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      661 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      721 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      781 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
      841 tatgcgatgg agtttcccca tactgagtgg gtggagactg aagttaggcc agcttggcac
      901 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
      961 cctcagacag tggttcaaag tttttttctt ccatttaagg tgtcgtgaaa actaccccaa
     1021 gctggcctct gcggccgcca ccatggtaaa cagctacaat agactggagg cgatatcaac
     1081 tcttgttata ctactctggg taactctggt ccagggttcc tgtggcagct gtccagctgg
     1141 tactttctgg aatggtacag attgtggaaa aaacaaaaat caagcttgca tcccatgtcc
     1201 cccaaatagt ttctctagta cagctggcga acagaggaca tgtaacatat gcaggagatg
     1261 tgaaggtatt tttagaacaa agagagaatg tactccaaca agtaatacag agtgtgattg
     1321 tatgtctggg tatcattgtt ccggagtagg atgtaatagg tgtcaaccag attgcaagca
     1381 aggtcaagaa cttctaggaa aaggttgtag agactgtagc tttggtacct ttaatgatca
     1441 gacacggggg aactgcaaac gctggacaaa ctgctctttg aatggactag ttgtgcttgt
     1501 aaatggaacc aataaaagag atgtgatatg tggatccacc caaacagttg ccactctgga
     1561 cacaatctcc atgacaattc cagcttctct cgtgcacatt gataagaacc atcagcccgg
     1621 ggagaacctt tacttccagg gatccgctgg ctccgctgct ggttctggcg aattccatca
     1681 tcaccatcac catgtcgacg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1741 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1801 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1861 cgtgccctgg cccaccctcg tgaccaccct gagctggggc gtgcagtgct tcgcccgcta
     1921 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1981 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2041 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2101 caacatcctg gggcacaagc tggagtacaa cgccatccac ggcaacgtct atatcaccgc
     2161 cgacaagcag aagaacggca tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg
     2221 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2281 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     2341 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2401 cgagctgtac aagtaggcta gcgtcaggcc aagcttgggg atcaattctc tagagctcgc
     2461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2641 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     2701 tctgaggcgg aaagaaccag ctgggcctac tagatagcgg accccttacc gaaacatcgc
     2761 cgcattctgc agaggagtcg aacttagtgt atgtaaactt ctgacccact ggaattgtga
     2821 tacagtgaat tataagtgaa ataatctgtc tgtaaacaat tgttggaaaa atgacttgtg
     2881 tcatgcacaa agtagatgtc ctaactgact tgccaaaact attgtttgtt aacaagaaat
     2941 ttgtggagta gttgaaaaac gagttttaat gactccaact taagtgtatg taaacttccg
     3001 acttcaactg tatagggatc caattttaac aaaatattcg agcttgcatg cctgcaggtc
     3061 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     3421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     3481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     3541 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     3601 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
     3661 gaggatccgg tactcgagga actgaaaaac cagaaagtta actggtaagt ttagtctttt
     3721 tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt
     3781 ggatgttgcc tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg
     3841 aattgtaccc gcggaattaa tacgactcac tatagggact agtaccatgg gaaaatcaaa
     3901 agaaatcagc caagacctca gaaaaagaat tgtagacctc cacaagtctg gttcatcctt
     3961 gggagcaatt tccaaacgcc tggcggtacc acgttcatct gtacaaacaa tagtacgcaa
     4021 gtataaacac catgggacca cgcagccgtc ataccgctca ggaaggagac gcgttctgtc
     4081 tcctagagat gaacgtactt tggtgcgaaa agtgcaaatc aatcccagaa caacagcaaa
     4141 ggaccttgtg aagatgctgg aggaaacagg tacaaaagta tctatatcca cagtaaaacg
     4201 agtcctatat cgacataacc tgaaaggcca ctcagcaagg aagaagccac tgctccaaaa
     4261 ccgacataag aaagccagac tacggtttgc aactgcacat ggggacaaag atcgtacttt
     4321 ttggagaaat gtcctctggt ctgatgaaac aaaaatagaa ctgtttggcc ataatgacca
     4381 tcgttatgtt tggaggaaga agggggaggc ttgcaagccg aagaacacca tcccaaccgt
     4441 gaagcacggg ggtggcagca tcatgttgtg ggggtgcttt gctgcaggag ggactggtgc
     4501 acttcacaaa atagatggca tcatggacgc ggtgcagtat gtggatatat tgaagcaaca
     4561 tctcaagaca tcagtcagga agttaaagct tggtcgcaaa tgggtcttcc aacacgacaa
     4621 tgaccccaag catacttcca aagttgtggc aaaatggctt aaggacaaca aagtcaaggt
     4681 attggagtgg ccatcacaaa gccctgacct caatcctata gaaaatttgt gggcagaact
     4741 gaaaaagcgt gtgcgagcaa ggaggcctac aaacctgact cagttacacc agctctgtca
     4801 ggaggaatgg gccaaaattc acccaaatta ttgtgggaag cttgtggaag gctacccgaa
     4861 acgtttgacc caagttaaac aatttaaagg caatgctacc aaatactagg ggccctaacc
     4921 gcggggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4981 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5041 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5101 gggaggtgtg ggaggttttt taacgcttac aatttcctga tgcggtattt tctccttacg
     5161 catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg tgcgcggaac
     5221 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5281 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5341 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5401 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5461 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5521 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5581 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5641 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5701 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5761 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5821 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5881 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5941 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6001 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6061 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6121 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6181 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6481 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6961 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7021 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7081 gaccgagcgc agcgagtcag tgagcgagga agcggatccc tatacagttg aagtcggaag
     7141 tttacataca ccttagccaa atacatttaa actcactttt tcacaattcc tgacatttaa
     7201 tcctagtaaa aattccctgt cttaggtcag ttaggatcac cactttattt taagaatgtg
     7261 aaatatcaga ataatagtag agagaatgat tcatttcagc ttttatttct ttcatcacat
     7321 tcccagtggg tcagaagttt acatacactc gactcctctg cagaatgcgg cgatgtttcg
     7381 gtaaggggtc ccgagtagct agttcatggc agccagcata tggcatatgt tgccaaactc
     7441 taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt
     7501 gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca
     7561 gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga
     7621 tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca
     7681 gcctgcacct gaggagtgaa ttcacgacag gccttcgaac tattcctttg ccctcggacg
     7741 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     7801 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     7861 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     7921 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     7981 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     8041 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     8101 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     8161 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     8221 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     8281 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     8341 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     8401 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     8461 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
     8521 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     8581 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     8641 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     8701 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatggtgg cctcaggtgc
     8761 agaggtttct acagggaaag agagaaaggc tacagttagt ttgctgtgat gatgctaagt
     8821 gccgaggtca gatgtctatg gattgtacac ggaatcccaa cgcaggaacc aaaaaacact
     8881 cacccaaaaa aatggcgcta aggccgagag aaaggcgaag ttccgtctac ggctatatac
     8941 cttgaaacag gggcggcaga agtgacgcaa cgcggttgcg cggacggctt cctgtgtttt
     9001 cattggtcga agcgttctgc gcgtgcgcag agattcccca gggaaggcgg gccctcttcc
     9061 gaatgtccaa tccggacatt cttccggttg gacctccgcg gtcagcgctg tgcccggcga
     9121 gagatcacgt ggggcgcgga ggcggtgctg ctggggcacg gccgtccagc ctcggcggcc
     9181 atatttttga ggggctgttc atctcgttca cacgctctgt ccgccatgtt tgtgagtgga
     9241 agcgccatta ccccttcaag cgactgaagg ctgcagggcc tctggtggcc cgcatgggga
     9301 gaccagaccc gccaggcccg cctttccgca ctcagtccgg gcttacttta ttttgtgaga
     9361 cagggtctcg cctagaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     9421 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     9481 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     9541 accgtatata a
//
