LOCUS       sequence-270084-        9566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF163
FEATURES             Location/Qualifiers
     source          1..9566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1211..1220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1796..1816
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1850..1867
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1874..2605
                     /codon_start=1
                     /product="far-red fluorescent protein with spectra similar 
                     to those of mNeptune but improved brightness"
                     /label=mNeptune2
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGR
                     IKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCDM
                     ALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(2657..2674)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2663..2887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3249..3552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3553..3756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3707..3727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3753..3777
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3894..3990
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     4047..4066
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4047..4065
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4076..5098
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    5115..5249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5139..5158)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5193..5212
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5402..5506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5507..5575,5576..6367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5725..5744)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6538..7126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7027..7046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7280..7297
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7671..7690
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7689..7744
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7790..7809
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7909..8934)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7909..8934)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        join(9566,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccatactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt aaggtgtcgt gaaaactacc ccaagctggc
     1201 ctctgcggcc gccaccatgg taaacagcta caatagactg gaggcgatat caactcttgt
     1261 tatactactc tgggtaactc tggtccaggg ttcctgtggc agctgtccag ctggtacttt
     1321 ctggaatggt acagattgtg gaaaaaacaa aaatcaagct tgcatcccat gtcccccaaa
     1381 tagtttctct agtacagctg gcgaacagag gacatgtaac atatgcagga gatgtgaagg
     1441 tatttttaga acaaagagag aatgtactcc aacaagtaat acagagtgtg attgtatgtc
     1501 tgggtatcat tgttccggag taggatgtaa taggtgtcaa ccagattgca agcaaggtca
     1561 agaacttcta ggaaaaggtt gtagagactg tagctttggt acctttaatg atcagacacg
     1621 ggggaactgc aaacgctgga caaactgctc tttgaatgga ctagttgtgc ttgtaaatgg
     1681 aaccaataaa agagatgtga tatgtggatc cacccaaaca gttgccactc tggacacaat
     1741 ctccatgaca attccagctt ctctcgtgca cattgataag aaccatcagc ccggggagaa
     1801 cctttacttc cagggatccg ctggctccgc tgctggttct ggcgaattcc atcatcacca
     1861 tcaccatgtc gacgtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct
     1921 gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
     1981 gccctacgag ggcacccaga ccggcagaat caaggtggtc gagggcggcc ctctcccctt
     2041 cgccttcgac atcctggcta cctgcttcat gtacggcagc aagaccttca tcaaccacac
     2101 ccagggcatc cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt
     2161 caccacatac gaagacgggg gcgtgcttac cgttacccag gacaccagcc tccaggacgg
     2221 ctgcttgatc tacaacgtca agctcagagg ggtgaacttc ccatccaacg gccctgtgat
     2281 gcagaagaaa acactcggct gggaggccag taccgagacc ctgtaccccg ctgacggcgg
     2341 cctggaaggc agatgcgaca tggccctgaa gctcgtgggc gggggccacc tgcactgcaa
     2401 cctgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta
     2461 ctttgtggac cgcagactgg aaagaatcaa ggaggccgac aatgagacct acgtcgagca
     2521 gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa
     2581 tggcatggac gagctgtaca agtaggctag cgtcaggcca agcttgggga tcaattctct
     2641 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2881 tctatggctt ctgaggcgga aagaaccagc tgggcctact agatagcgga ccccttaccg
     2941 aaacatcgcc gcattctgca gaggagtcga acttagtgta tgtaaacttc tgacccactg
     3001 gaattgtgat acagtgaatt ataagtgaaa taatctgtct gtaaacaatt gttggaaaaa
     3061 tgacttgtgt catgcacaaa gtagatgtcc taactgactt gccaaaacta ttgtttgtta
     3121 acaagaaatt tgtggagtag ttgaaaaacg agttttaatg actccaactt aagtgtatgt
     3181 aaacttccga cttcaactgt atagggatcc aattttaaca aaatattcga gcttgcatgc
     3241 ctgcaggtcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     3601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     3661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3721 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     3781 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     3841 cggactctag aggatccggt actcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt
     3901 tagtcttttt gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg
     3961 ctcctcagtg gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa
     4021 aagctgcgga attgtacccg cggaattaat acgactcact atagggacta gtaccatggg
     4081 aaaatcaaaa gaaatcagcc aagacctcag aaaaagaatt gtagacctcc acaagtctgg
     4141 ttcatccttg ggagcaattt ccaaacgcct ggcggtacca cgttcatctg tacaaacaat
     4201 agtacgcaag tataaacacc atgggaccac gcagccgtca taccgctcag gaaggagacg
     4261 cgttctgtct cctagagatg aacgtacttt ggtgcgaaaa gtgcaaatca atcccagaac
     4321 aacagcaaag gaccttgtga agatgctgga ggaaacaggt acaaaagtat ctatatccac
     4381 agtaaaacga gtcctatatc gacataacct gaaaggccac tcagcaagga agaagccact
     4441 gctccaaaac cgacataaga aagccagact acggtttgca actgcacatg gggacaaaga
     4501 tcgtactttt tggagaaatg tcctctggtc tgatgaaaca aaaatagaac tgtttggcca
     4561 taatgaccat cgttatgttt ggaggaagaa gggggaggct tgcaagccga agaacaccat
     4621 cccaaccgtg aagcacgggg gtggcagcat catgttgtgg gggtgctttg ctgcaggagg
     4681 gactggtgca cttcacaaaa tagatggcat catggacgcg gtgcagtatg tggatatatt
     4741 gaagcaacat ctcaagacat cagtcaggaa gttaaagctt ggtcgcaaat gggtcttcca
     4801 acacgacaat gaccccaagc atacttccaa agttgtggca aaatggctta aggacaacaa
     4861 agtcaaggta ttggagtggc catcacaaag ccctgacctc aatcctatag aaaatttgtg
     4921 ggcagaactg aaaaagcgtg tgcgagcaag gaggcctaca aacctgactc agttacacca
     4981 gctctgtcag gaggaatggg ccaaaattca cccaaattat tgtgggaagc ttgtggaagg
     5041 ctacccgaaa cgtttgaccc aagttaaaca atttaaaggc aatgctacca aatactaggg
     5101 gccctaaccg cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     5161 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5221 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5281 aggttcaggg ggaggtgtgg gaggtttttt aacgcttaca atttcctgat gcggtatttt
     5341 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
     5401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5461 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5701 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6001 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6121 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6181 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6241 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6301 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6361 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6661 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggatccct atacagttga
     7321 agtcggaagt ttacatacac cttagccaaa tacatttaaa ctcacttttt cacaattcct
     7381 gacatttaat cctagtaaaa attccctgtc ttaggtcagt taggatcacc actttatttt
     7441 aagaatgtga aatatcagaa taatagtaga gagaatgatt catttcagct tttatttctt
     7501 tcatcacatt cccagtgggt cagaagttta catacactcg actcctctgc agaatgcggc
     7561 gatgtttcgg taaggggtcc cgagtagcta gttcatggca gccagcatat ggcatatgtt
     7621 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
     7681 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
     7741 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
     7801 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
     7861 tgataggcag cctgcacctg aggagtgaat tcacgacagg ccttcgaact attcctttgc
     7921 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     7981 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     8041 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     8101 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     8161 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     8221 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     8281 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     8341 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     8401 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     8461 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     8521 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     8581 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     8641 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     8701 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     8761 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     8821 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
     8881 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatggtggc
     8941 ctcaggtgca gaggtttcta cagggaaaga gagaaaggct acagttagtt tgctgtgatg
     9001 atgctaagtg ccgaggtcag atgtctatgg attgtacacg gaatcccaac gcaggaacca
     9061 aaaaacactc acccaaaaaa atggcgctaa ggccgagaga aaggcgaagt tccgtctacg
     9121 gctatatacc ttgaaacagg ggcggcagaa gtgacgcaac gcggttgcgc ggacggcttc
     9181 ctgtgttttc attggtcgaa gcgttctgcg cgtgcgcaga gattccccag ggaaggcggg
     9241 ccctcttccg aatgtccaat ccggacattc ttccggttgg acctccgcgg tcagcgctgt
     9301 gcccggcgag agatcacgtg gggcgcggag gcggtgctgc tggggcacgg ccgtccagcc
     9361 tcggcggcca tatttttgag gggctgttca tctcgttcac acgctctgtc cgccatgttt
     9421 gtgagtggaa gcgccattac cccttcaagc gactgaaggc tgcagggcct ctggtggccc
     9481 gcatggggag accagacccg ccaggcccgc ctttccgcac tcagtccggg cttactttat
     9541 tttgtgagac agggtctcgc ctagag
//
