LOCUS       sequence-27010-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control Lentiviral vector with ubiquitous PGK promoter, expresses 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, 
            Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
  TITLE     Isolation of human iPS cells using EOS lentiviral vectors to select 
            for pluripotency.
  JOURNAL   Nat Methods. 2009 May . 6(5):370-6.
  PUBMED    19404254
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 May . 6(5):370-6."
COMMENT     Sequence Label: PL-SIN-PGK-EGFP
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(41..540)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(89..108)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     482..505
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggtgacgctc ctcgcccttg ctcaccatgg tggctgcagc cgaaaggccc ggagatgagg
       61 aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg
      121 aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac
      181 cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc
      241 aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac
      301 gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc
      361 tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc
      421 gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc
      481 ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc
      541 ggtagaatta gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
      601 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
      661 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
      721 taaacctacc gaggcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
      781 gattccnaat ctgtttccag anatttattt actnccnact agtcattccn aggcaca
//
