LOCUS       sequence-27011-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoMLV-based Retroviral vector, expresses mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, 
            Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
  TITLE     Isolation of human iPS cells using EOS lentiviral vectors to select 
            for pluripotency.
  JOURNAL   Nat Methods. 2009 May . 6(5):370-6.
  PUBMED    19404254
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 May . 6(5):370-6."
COMMENT     Sequence Label: pMXs-mRFP1
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             65..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(195..213)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     463..482
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     657..680
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ggggtggacc atcctctaga ctgccggatc tagctagtta attaaggatc caccggtcgc
       61 caccatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
      121 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      181 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
      241 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
      301 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      361 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      421 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      481 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
      541 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
      601 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
      661 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
      721 ccgccactcc accggcgcct aatacngccg ctcgataagc ttgatgacga taaaataaaa
      781 gattttattt agtctccana aaaaaggggg ggaatgaaag accccnaccc tgntagggtt
      841 tggg
//
