LOCUS       sequence-27012-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and 
            Puro resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, 
            Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
  TITLE     Isolation of human iPS cells using EOS lentiviral vectors to select 
            for pluripotency.
  JOURNAL   Nat Methods. 2009 May . 6(5):370-6.
  PUBMED    19404254
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 May . 6(5):370-6."
COMMENT     Sequence Label: PL-SIN-EOS-S(4+)-EiP
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggctt taccaacagt accggaatgc caagcttact
       61 tagatcgcag atcttgttgc ggccgccagc agctcgcaac gtgaacggtt cgactgagaa
      121 ggccgctcga gctgtaagag aggaatctag acggggcgga agaaagaatg tagccaagac
      181 agttactctg atcaaggctc aaattttata gttgcgacac tagttatgaa ggaaggggga
      241 ggggacccga ttcccgccga ataatctctg gtccagtaga aaggtgcacg ggtgtggctc
      301 cgcaggttcc agcagtgggc gtggcagaac gaatgagcag gaagctccac ccctgagcaa
      361 gcaggtttca ggctggggga ggggagaatt cgattctact cgagagcaag aactgtcgac
      421 tgtgctcatt accacgtgaa taatcctata tgcatcacaa tggctgcccg agcccgggaa
      481 attcttttag agtctctgca ttaattagag gggaacctgc ctagcttgga ctgggaattc
      541 gattctactc gagagcaaga actgtcgact gtgctcatta ccacgtgaat aatcctatat
      601 gcatcacaat ggctgcccga gcccgggaaa ttcttttaga gtctctgcat taattagagg
      661 ggaacctgcc tagcttggac tgggaattcg attctactcg agagcaagaa ctgtcgactg
      721 tgctcattac cacgtgaata atcctatatg catcac
//
