LOCUS       sequence-270125-        9551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF138
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             614..634
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             668..685
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(692..694,695..1408)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(995..1014)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(1460..1477)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1466..1690
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2052..2355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2356..2559
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2510..2530
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2556..2580
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2697..2793
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     2850..2869
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2850..2868
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2879..3901
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    3918..4052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3942..3961)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3996..4015
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4205..4309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4310..4378,4379..5170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4528..4547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5341..5929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5830..5849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6083..6100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6474..6493
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6492..6547
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6593..6612
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6712..7737)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6712..7737)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        8388..8599
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     9499..9519
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aaactacccc aagctggcct ctgcggccgc caccatggta aacagctaca atagactgga
       61 ggcgatatca actcttgtta tactactctg ggtaactctg gtccagggtt cctgtggcag
      121 ctgtccagct ggtactttct ggaatggtac agattgtgga aaaaacaaaa atcaagcttg
      181 catcccatgt cccccaaata gtttctctag tacagctggc gaacagagga catgtaacat
      241 atgcaggaga tgtgaaggta tttttagaac aaagagagaa tgtactccaa caagtaatac
      301 agagtgtgat tgtatgtctg ggtatcattg ttccggagta ggatgtaata ggtgtcaacc
      361 agattgcaag caaggtcaag aacttctagg aaaaggttgt agagactgta gctttggtac
      421 ctttaatgat cagacacggg ggaactgcaa acgctggaca aactgctctt tgaatggact
      481 agttgtgctt gtaaatggaa ccaataaaag agatgtgata tgtggatcca cccaaacagt
      541 tgccactctg gacacaatct ccatgacaat tccagcttct ctcgtgcaca ttgataagaa
      601 ccatcagccc ggggagaacc tttacttcca gggatccgct ggctccgctg ctggttctgg
      661 cgaattccat catcaccatc accatgtcga cgtgagcaag ggcgaggagc tgttcaccgg
      721 cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag
      781 cggcgagggc gagggcgacg ccacctacgg caagttgacc ctgaagttca tctgcaccac
      841 cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ttcggctacg gcctgatgtg
      901 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga
      961 gggctacgtg caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1021 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1081 caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt
     1141 gtacatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1201 catcgaggac ggcagcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga
     1261 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagagcaagc tgagcaagga
     1321 ccccaacgag aagcgcgacc acatggtgct gctggagttc gtgaccgccg ccggcatcac
     1381 cctgggcatg gacgagctgt acaagtaggc tagcgtcagg ccaagcttgg ggatcaattc
     1441 tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     1501 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     1561 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     1621 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     1681 ggctctatgg cttctgaggc ggaaagaacc agctgggcct actagatagc ggacccctta
     1741 ccgaaacatc gccgcattct gcagaggagt cgaacttagt gtatgtaaac ttctgaccca
     1801 ctggaattgt gatacagtga attataagtg aaataatctg tctgtaaaca attgttggaa
     1861 aaatgacttg tgtcatgcac aaagtagatg tcctaactga cttgccaaaa ctattgtttg
     1921 ttaacaagaa atttgtggag tagttgaaaa acgagtttta atgactccaa cttaagtgta
     1981 tgtaaacttc cgacttcaac tgtataggga tccaatttta acaaaatatt cgagcttgca
     2041 tgcctgcagg tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     2101 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     2161 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2221 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2281 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2341 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2401 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2461 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2521 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     2581 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     2641 ctccggactc tagaggatcc ggtactcgag gaactgaaaa accagaaagt taactggtaa
     2701 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
     2761 ctgctcctca gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc
     2821 taaaagctgc ggaattgtac ccgcggaatt aatacgactc actataggga ctagtaccat
     2881 gggaaaatca aaagaaatca gccaagacct cagaaaaaga attgtagacc tccacaagtc
     2941 tggttcatcc ttgggagcaa tttccaaacg cctggcggta ccacgttcat ctgtacaaac
     3001 aatagtacgc aagtataaac accatgggac cacgcagccg tcataccgct caggaaggag
     3061 acgcgttctg tctcctagag atgaacgtac tttggtgcga aaagtgcaaa tcaatcccag
     3121 aacaacagca aaggaccttg tgaagatgct ggaggaaaca ggtacaaaag tatctatatc
     3181 cacagtaaaa cgagtcctat atcgacataa cctgaaaggc cactcagcaa ggaagaagcc
     3241 actgctccaa aaccgacata agaaagccag actacggttt gcaactgcac atggggacaa
     3301 agatcgtact ttttggagaa atgtcctctg gtctgatgaa acaaaaatag aactgtttgg
     3361 ccataatgac catcgttatg tttggaggaa gaagggggag gcttgcaagc cgaagaacac
     3421 catcccaacc gtgaagcacg ggggtggcag catcatgttg tgggggtgct ttgctgcagg
     3481 agggactggt gcacttcaca aaatagatgg catcatggac gcggtgcagt atgtggatat
     3541 attgaagcaa catctcaaga catcagtcag gaagttaaag cttggtcgca aatgggtctt
     3601 ccaacacgac aatgacccca agcatacttc caaagttgtg gcaaaatggc ttaaggacaa
     3661 caaagtcaag gtattggagt ggccatcaca aagccctgac ctcaatccta tagaaaattt
     3721 gtgggcagaa ctgaaaaagc gtgtgcgagc aaggaggcct acaaacctga ctcagttaca
     3781 ccagctctgt caggaggaat gggccaaaat tcacccaaat tattgtggga agcttgtgga
     3841 aggctacccg aaacgtttga cccaagttaa acaatttaaa ggcaatgcta ccaaatacta
     3901 ggggccctaa ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     3961 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4021 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4081 ttcaggttca gggggaggtg tgggaggttt tttaacgctt acaatttcct gatgcggtat
     4141 tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa
     4201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6061 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
     6121 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
     6181 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
     6241 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
     6301 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     6361 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     6421 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     6481 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     6541 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     6601 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     6661 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     6721 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     6781 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     6841 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     6901 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     6961 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     7021 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     7081 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     7141 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     7201 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     7261 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     7321 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     7381 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     7441 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     7501 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     7561 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     7621 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     7681 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     7741 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     7801 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     7861 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     7921 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     7981 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     8041 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     8101 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     8161 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     8221 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     8281 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     8341 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     8401 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     8461 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     8521 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     8581 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     8641 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     8701 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     8761 ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa
     8821 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     8881 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     8941 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     9001 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     9061 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     9121 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     9181 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     9241 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     9301 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     9361 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     9421 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     9481 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     9541 aggtgtcgtg a
//
