LOCUS       sequence-270126-        7984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A2-VN (Y59P)
FEATURES             Location/Qualifiers
     source          1..7984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(173..191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        259..363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(364..432,433..1224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(582..601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1395..1983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1884..1903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2414..2559
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2602..3639
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3637..3656)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3681..3729
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4383..4762
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4763..4966
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4917..4937
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4963..4987
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5069..5098
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6146..6148,6149..6607)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6188..6209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6449..6468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    6630..6764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6654..6673)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6708..6727
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6907..6929)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
       61 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
      121 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
      181 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      241 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      301 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      361 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      421 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      481 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      541 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      601 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      661 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      721 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      781 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      841 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
      901 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
      961 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1021 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1081 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1141 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1201 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1261 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1321 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1381 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1441 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1501 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1561 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1621 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1681 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1741 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1801 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1861 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1921 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1981 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     2041 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     2101 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     2161 agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg
     2221 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     2281 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat
     2341 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     2401 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     2461 gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt
     2521 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
     2581 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     2641 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     2701 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     2761 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     2821 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     2881 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     2941 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     3001 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     3061 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     3121 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     3181 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     3241 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     3301 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     3361 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     3421 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     3481 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     3541 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     3601 agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc
     3661 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     3721 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     3781 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     3841 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
     3901 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
     3961 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
     4021 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
     4081 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
     4141 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
     4201 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
     4261 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     4321 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
     4381 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     4441 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4501 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4561 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4621 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
     4681 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
     4741 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
     4801 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4861 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     4921 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     4981 tcagatctct agaagctggg taccagctgc tagcgccacc atgaagacca tcatcgccct
     5041 gagctacatc ttctgcctgg tgttcgccga gcagaaattg atcagcgagg aggacctggg
     5101 cagccctgga ggcagttcag gaggcggatc tcactccatg aggtatttct tcacatccgt
     5161 gtcccggccc ggccgcgggg agccccgctt catcgcagtg ggctacgtgg acgacacgca
     5221 gttcgtgcgg ttcgacagcg acgccgcgag ccagaggatg gagccgcggg cgccgtggat
     5281 agagcaggag ggtccggagc cttgggacgg ggagacacgg aaagtgaagg cccactcaca
     5341 gactcaccga gtggacctgg ggaccctgcg cggctactac aaccagagcg aggccggttc
     5401 tcacaccgtc cagaggatgt atggctgcga cgtggggtcg gactggcgct tcctccgcgg
     5461 gtaccaccag tacgcctacg acggcaagga ttacatcgcc ctgaaagagg acctgcgctc
     5521 ttggaccgcg gcggacatgg cagctcagac caccaagcac aagtgggagg cggcccatgt
     5581 ggcggagcag ttgagagcct acctggaggg cacgtgcgtg gagtggctcc gcagatacct
     5641 ggagaacggg aaggagacgc tgcagcgtac ggacgccccc aaaacgcata tgactcacca
     5701 cgctgtctct gaccatgaag ccaccctgag gtgctgggcc ctgagcttct accctgcgga
     5761 gatcacactg acctggcagc gggatgggga ggaccagacc caggacacgg agctcgtgga
     5821 gaccaggcct gcaggggatg gaaccttcca gaagtgggcg gctgtggtgg tgccttctgg
     5881 acaggagcag agatacacct gccatgtgca gcatgagggt ttgcccaagc ccctcaccct
     5941 gagatgggag ccgtcttccc agcccaccat ccccatcgtg ggcatcattg ctggcctggt
     6001 tctctttgga gctgtgatca ctggagctgt ggtcgctgct gtgatgtgga ggaggaagag
     6061 ctcagataga aaaggaggga gctactctca ggctgcaagc agtgacagtg cccagggctc
     6121 tgatgtgtct ctcacagctt gtaaagtgag caagggcgag gagctgttca ccggggtggt
     6181 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6241 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     6301 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     6361 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6421 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6481 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6541 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatct
     6601 gaccgcctaa ctcgaggccg gcaaggccgg atccagacat gataagatac attgatgagt
     6661 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     6721 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     6781 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     6841 tctacaaatg tggtatggct gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg
     6901 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6961 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     7021 catgaggtcg actctagagg atcgatcccc gccccggacg aactaaacct gactacgaca
     7081 tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg
     7141 ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg
     7201 actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat
     7261 cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct
     7321 tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact
     7381 acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct
     7441 caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat
     7501 atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta
     7561 cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga
     7621 tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg
     7681 tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc
     7741 tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag
     7801 ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag
     7861 aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc
     7921 acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca
     7981 atag
//
