LOCUS       sequence-27014-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control Lentiviral vector with ubiquitous PGK promoter, expresses 
            EGFP and Puro resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, 
            Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
  TITLE     Isolation of human iPS cells using EOS lentiviral vectors to select 
            for pluripotency.
  JOURNAL   Nat Methods. 2009 May . 6(5):370-6.
  PUBMED    19404254
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 May . 6(5):370-6."
COMMENT     Sequence Label: PL-SIN-PGK-EiP
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(38..537)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(86..105)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     479..502
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gacgctcctc gcccttgctc accatggtgg ctgcagccga aaggcccgga gatgaggaag
       61 aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg
      121 accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc
      181 ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa
      241 ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg
      301 ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga
      361 ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc
      421 taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca
      481 gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
      541 agaattagat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
      601 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
      661 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
      721 acctaccaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
      781 ccaaatctgt tccaganntt tattactcca actagcattt cca
//
