LOCUS       sequence-27016-e        1216 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, 
            Gray NS, Sabatini DM
  TITLE     DEPTOR is an mTOR inhibitor frequently overexpressed in multiple 
            myeloma cells and required for their survival.
  JOURNAL   Cell. 2009 May 29;137(5):873-86. doi: 10.1016/j.cell.2009.03.046. 
            Epub 2009 May 14.
  PUBMED    19446321
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.03.046"; journalName: "Cell"; date: 
            "2009-05-29- 29"; volume: "137"; issue: "5"; pages: "873-86"
COMMENT     Sequence Label: pLKO mouse shRNA 2 DEPTOR
FEATURES             Location/Qualifiers
     source          1..1216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    141..258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        307..817
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     736..754
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(840..859)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     gap             902..930
                     /estimated_length=29
     gap             933..957
                     /estimated_length=25
     gap             972..983
                     /estimated_length=12
     gap             996..1015
                     /estimated_length=20
     gap             1110..1119
                     /estimated_length=10
     gap             1177..1187
                     /estimated_length=11
ORIGIN
        1 cnattgattc ttggcttttt atcttgtgga aaggacgaaa caccgggcaa ggaagacatt
       61 cacgattctc gagaatcgtg aatgtcttcc ttgctttttg aattctcgac ctcgagacaa
      121 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
      181 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
      241 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg
      301 ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
      361 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
      421 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
      481 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
      541 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
      601 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
      661 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
      721 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
      781 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccacccg gagcttacca
      841 tgaccgagta caagcccacg gtgcgcctcg caccggnact ccagcnnnct nccntcnaac
      901 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccnnnnnnnn nnnnnnnnnn nnnnnnncnc
      961 nnttgggggg gnnnnnnnnn nnnaantttt nnnccnnnnn nnnnnnnnnn nnnnnantnn
     1021 nnannngggn nnngnanaan nccccccnnn nnntttnnnn cncccccnaa annnnaanan
     1081 ngggggggnt tttccaaaan nnnccccncn nnnnnnnnnt tnnnnntncc nnnngncccn
     1141 nnnnaacnaa aaaannnnnt nggntnccnc nnnnnannnn nnnnnnntnt ngnncnnnnn
     1201 ngnntnnncc cnnnca
//
