LOCUS       sequence-270160-       11739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-A*24:02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A24-VN
FEATURES             Location/Qualifiers
     source          1..11739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1764..1766,1767..2225)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(1806..1827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2067..2086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2248..2382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2272..2291)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2326..2345
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2525..2547)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3107..4896
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(7530..7548)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7616..7720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7721..7789,7790..8581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7939..7958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8752..9340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9241..9260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9771..9916
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9959..10996
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10994..11013)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11038..11086
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cagttcagga ggcggatctc attctatgag atatttctcc acatccgtgt
      781 cccggcccgg ccgcggggag ccccgcttca tcgccgtggg ctacgtggac gacacgcagt
      841 tcgtgcggtt cgacagcgac gccgcgagcc agaggatgga gccgcgggct ccgtggatag
      901 agcaggaggg gccggagtat tgggacgagg agacagggaa agtgaaggcc cactcacaga
      961 ctgaccgaga gaacctgcgg atcgcgctcc gctactacaa ccagagcgag gccggttctc
     1021 acaccctcca gatgatgttt ggctgcgacg tggggtcgga cgggcgcttc ctccgcgggt
     1081 accaccagta cgcctacgac ggcaaggatt acatcgccct gaaagaggac ctgcgctctt
     1141 ggaccgcggc ggacatggcg gctcagatca ccaagcgcaa gtgggaggcg gcccatgtgg
     1201 cggagcagca gagagcctac ctggagggca cgtgcgtgga cgggctccgc agatacctgg
     1261 agaacgggaa ggagacgctg cagcgcacgg acccccccaa gacacatatg acccaccacc
     1321 ccatctctga ccatgaggcc actctgagat gctgggccct gggcttctac cctgcggaga
     1381 tcacactgac ctggcagcgg gatggggagg accagaccca ggacacggag cttgtggaga
     1441 ccaggcctgc aggggatgga accttccaga agtgggcagc tgtggtggta ccttctggag
     1501 aggagcagag atacacctgc catgtgcagc atgagggtct gcccaagccc ctcaccctga
     1561 gatgggagcc atcttcccag cccacggtgc ccatcgtagg cataatagct ggccttgtgc
     1621 tccttggggc cgtaataact ggggcagttg tcgccgccgt gatgtggcgg agaaacagta
     1681 gtgatcgaaa aggcggtagc tattcccaag cagcgagtag cgattctgcc caggggagtg
     1741 acgtgtcact gacggcctgt aaagtgagca agggcgagga gctgttcacc ggggtggtgc
     1801 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1861 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     1921 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
     1981 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2041 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2101 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2161 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatctga
     2221 ccgcctaact cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     2281 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2341 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2401 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     2461 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     2521 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2581 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     2641 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     2701 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     2761 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     2821 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     2881 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     2941 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3001 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3061 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3121 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3181 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3241 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3301 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3361 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3421 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3481 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3541 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3601 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3661 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     3721 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     3781 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     3841 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     3901 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     3961 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4021 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4081 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4141 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4201 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4261 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4321 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4381 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4441 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4501 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4621 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4681 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4741 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
     4801 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
     4861 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
     4921 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     4981 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     5041 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     5101 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     5161 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     5221 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     5281 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     5341 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     5401 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     5461 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
     5521 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
     5581 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
     5641 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
     5701 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
     5761 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     5821 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     5881 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     5941 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     6001 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     6061 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     6121 cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc
     6181 ctcctgctcc tgcccctcct gcccctccts ctcctgcccc tcctccccct cctcctccys
     6241 cccctcctsc ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg
     6301 cccctcctgc tcctgcccct cctgccctcc tgctcctgcc tcctgcccct cctgctcctg
     6361 tccctcctyc tcctgtctcc ctgccrctyc tgcccctcct gctcctgccc ctcctgcccc
     6421 tcctcccccc cccgctccca cgcccccccc cgccccctcc ccaccgacgc ctgctacccc
     6481 accgacgcct actcctcctc ctacycmtgc cactactgcc gctcctgcyc ctcctcctgc
     6541 tcctgcccct cctgcccctc ctcctgctcc tscycctcct cctgctcctg cccctcctgc
     6601 ccctcctgcc cctcctcctg ctcctgcccc tcctgccccn nnncctgctc ctgcccctcc
     6661 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctcc
     6721 tcctcccccy cctccccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     6781 tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg
     6841 ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg
     6901 tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc
     6961 tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga
     7021 gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg
     7081 cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc
     7141 tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc
     7201 cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct
     7261 cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg
     7321 cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg
     7381 atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg
     7441 gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt
     7501 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7561 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     7981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     8281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9361 tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg
     9421 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     9481 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     9541 ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
     9601 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     9661 gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt
     9721 tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa
     9781 ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg
     9841 gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct
     9901 gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc aatgagatat
     9961 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
    10021 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
    10081 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
    10141 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
    10201 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
    10261 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
    10321 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
    10381 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
    10441 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
    10501 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
    10561 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
    10621 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
    10681 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
    10741 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
    10801 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
    10861 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
    10921 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg atcgggagat
    10981 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
    11041 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
    11101 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
    11161 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
    11221 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
    11281 cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc
    11341 aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg ggcatggacc
    11401 gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc cccgaccccc
    11461 aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc atgtttgaca
    11521 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
    11581 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
    11641 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
    11701 acatttatat tggctcatgt ccaatatgac cgccatgtt
//
