LOCUS       sequence-270162-       10624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myc-tagged HLA-A*03:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-A3
FEATURES             Location/Qualifiers
     source          1..10624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1792..1926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1816..1835)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1870..1889
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2069..2091)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6415..6433)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6501..6605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6606..6674,6675..7466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6824..6843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7637..8225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8126..8145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8656..8801
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8844..9881
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9879..9898)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9923..9971
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cagttcagga ggcggatctc attctatgag atatttcttc acatccgtgt
      781 cccggcccgg ccgcggggag ccccgcttca tcgccgtggg ctacgtggac gacacgcagt
      841 tcgtgcggtt cgacagcgac gccgcgagcc agaggatgga gccgcgggcg ccgtggatag
      901 agcaggaggg gccggagtat tgggaccagg agacacggaa tgtgaaggcc cagtcacaga
      961 ctgaccgagt ggacctgggg accctgcgcg gctactacaa ccagagcgag gccggttctc
     1021 acaccatcca gataatgtat ggctgcgacg tggggtcgga cgggcgcttc ctccgcgggt
     1081 accggcagga cgcctacgac ggcaaggatt acatcgccct gaacgaggac ctgcgctctt
     1141 ggaccgcggc ggacatggcg gctcagatca ccaagcgcaa gtgggaggcg gcccatgagg
     1201 cggagcagtt gagagcctac ctggatggca cgtgcgtgga gtggctccgc agatacctgg
     1261 agaacgggaa ggagacgctg cagcgcacgg acccccccaa gacacatatg acccaccacc
     1321 ccatctctga ccatgaggcc accctgaggt gctgggccct gggcttctac cctgcggaga
     1381 tcacactgac ctggcagcgg gatggggagg accagaccca ggacacggag ctcgtggaga
     1441 ccaggcctgc aggggatgga accttccaga agtgggcggc tgtggtggtg ccttctggag
     1501 aggagcagag atacacctgc catgtgcagc atgagggtct gcccaagccc ctcaccctga
     1561 gatgggagct gtcttcccag cccactatac cgattgttgg catcatagcc ggacttgtat
     1621 tgctgggtgc ggtgattacg ggcgccgtcg tcgctgctgt gatgtggcgg cggaagtctt
     1681 cagacagaaa aggcggatct tatacccaag ctgcaagctc agattcagca caggggtctg
     1741 atgtgagtct gacagcttgc aaagtgtgat aactcgaggc cggcaaggcc ggatccagac
     1801 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1861 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1921 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     1981 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc
     2041 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     2101 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     2161 ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc ccgccccgga
     2221 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     2281 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     2341 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     2401 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     2461 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     2521 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     2581 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     2641 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     2701 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     2761 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     2821 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     2881 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     2941 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     3001 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     3061 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     3121 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     3181 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     3241 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     3301 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     3361 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     3421 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     3481 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     3541 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     3601 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     3661 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     3721 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     3781 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     3841 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     3901 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     3961 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     4021 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     4081 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     4141 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     4201 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     4261 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
     4321 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
     4381 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
     4441 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
     4501 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
     4561 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
     4621 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
     4681 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
     4741 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
     4801 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
     4861 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
     4921 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
     4981 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
     5041 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
     5101 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
     5161 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
     5221 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
     5281 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
     5341 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
     5401 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
     5461 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
     5521 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
     5581 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
     5641 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcccgctc
     5701 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
     5761 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
     5821 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
     5881 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
     5941 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
     6001 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
     6061 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
     6121 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
     6181 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
     6241 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
     6301 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
     6361 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
     6421 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6601 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6661 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6721 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6781 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6841 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6901 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6961 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7021 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7081 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7141 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7201 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7261 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7321 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7381 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7441 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     8281 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     8341 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     8401 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     8461 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     8521 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     8581 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     8641 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     8701 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     8761 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     8821 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     8881 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     8941 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     9001 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     9061 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     9121 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     9181 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     9241 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     9301 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     9361 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     9421 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     9481 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     9541 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     9601 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     9661 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     9721 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     9781 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     9841 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     9901 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     9961 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
    10021 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
    10081 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
    10141 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
    10201 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
    10261 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
    10321 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
    10381 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
    10441 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
    10501 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
    10561 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
    10621 tgtt
//
