LOCUS       sequence-270164-       11110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-B*08:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B8-VN
FEATURES             Location/Qualifiers
     source          1..11110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1764..1766,1767..2225)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(1806..1827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2067..2086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2248..2382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2272..2291)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2326..2345
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2525..2547)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3107..4896
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6901..6919)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6987..7091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7092..7160,7161..7952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7310..7329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8123..8711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8612..8631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9142..9287
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9330..10367
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10365..10384)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10409..10457
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cagttcagga ggcggatctc attctatgag atatttcgac accgccatgt
      781 cccggcccgg ccgcggggag ccccgcttca tctcagtggg ctacgtggac gacacgcagt
      841 tcgtgaggtt cgacagcgac gccgcgagtc cgagagagga gccgcgggcg ccgtggatag
      901 agcaggaggg gccggagtat tgggaccgga acacacagat cttcaagacc aacacacaga
      961 ctgaccgaga gagcctgcgg aacctgcgcg gctactacaa ccagagcgag gccgggtctc
     1021 acaccctcca gagcatgtac ggctgcgacg tggggccgga cgggcgcctc ctccgcgggc
     1081 ataaccagta cgcctacgac ggcaaggatt acatcgccct gaacgaggac ctgcgctcct
     1141 ggaccgcggc ggacaccgcg gctcagatca cccagcgcaa gtgggaggcg gcccgtgtgg
     1201 cggagcagga cagagcctac ctggagggca cgtgcgtgga gtggctccgc agatacctgg
     1261 agaacgggaa ggacacgctg gagcgcgcgg accccccaaa gacacacgtg acccaccacc
     1321 ccatctctga ccatgaggcc accctgaggt gctgggccct gggcttctac cctgcggaga
     1381 tcacactgac ctggcagcgg gatggcgagg accaaactca ggacactgag cttgtggaga
     1441 ccagaccagc aggagataga accttccaga agtgggcagc tgtggtggtg ccttctggag
     1501 aagagcagag atacacatgc catgtacagc atgaggggct gccgaagccc ctcaccctga
     1561 gatgggagcc gtcttcccag tccactgttc ccatcgtcgg tatagttgct ggcctcgcag
     1621 tcttggcggt agtagtcatt ggagctgtag tggcagcagt gatgtgcaga cgcaagagtt
     1681 ccggcggcaa gggaggctcc tattctcaag ctgcttgctc cgactcagca caaggcagtg
     1741 atgtctcatt gactgcagga ggcgtgagca agggcgagga gctgttcacc ggggtggtgc
     1801 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1861 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     1921 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
     1981 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2041 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2101 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2161 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatctga
     2221 ccgcctaact cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     2281 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2341 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2401 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     2461 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     2521 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2581 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     2641 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     2701 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     2761 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     2821 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     2881 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     2941 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3001 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3061 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3121 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3181 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3241 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3301 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3361 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3421 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3481 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3541 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3601 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3661 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     3721 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     3781 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     3841 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     3901 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     3961 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4021 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4081 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4141 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4201 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4261 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4321 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4381 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4441 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4501 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4621 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4681 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4741 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
     4801 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
     4861 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
     4921 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
     4981 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
     5041 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
     5101 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
     5161 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
     5221 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
     5281 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
     5341 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
     5401 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
     5461 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
     5521 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
     5581 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
     5641 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
     5701 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
     5761 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
     5821 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
     5881 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
     5941 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
     6001 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
     6061 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
     6121 cactgcctcc tcgaccccgg cctccwcctc ctgctcctgc ccctcctgct cctgcccctc
     6181 cygctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
     6241 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
     6301 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
     6361 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
     6421 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
     6481 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
     6541 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
     6601 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
     6661 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
     6721 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
     6781 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
     6841 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     6901 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7321 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7621 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8761 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     8821 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     8881 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     8941 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     9001 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     9061 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     9121 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     9181 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     9241 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     9301 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     9361 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     9421 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     9481 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     9541 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     9601 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     9661 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     9721 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     9781 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     9841 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     9901 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     9961 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
    10021 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
    10081 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
    10141 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
    10201 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
    10261 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
    10321 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
    10381 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
    10441 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
    10501 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
    10561 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
    10621 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
    10681 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
    10741 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
    10801 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
    10861 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
    10921 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
    10981 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
    11041 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
    11101 ccgccatgtt
//
