LOCUS       sequence-270165-        9542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF164
FEATURES             Location/Qualifiers
     source          1..9542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        484..1666
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     1614..1634
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1695..1704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2280..2300
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2334..2351
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2358..3065
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     2768..2787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3117..3134)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3123..3347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3709..4012
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4013..4216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4167..4187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4213..4237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4354..4450
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     4507..4526
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4507..4525
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4536..5558
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    5575..5709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5599..5618)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5653..5672
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5862..5966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5967..6035,6036..6827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6185..6204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6998..7586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7487..7506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7740..7757
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8131..8150
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    8149..8204
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     8250..8269
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(8369..9394)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8369..9394)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ggcgctaagg ccgagagaaa ggcgaagttc cgtctacggc tatatacctt gaaacagggg
       61 cggcagaagt gacgcaacgc ggttgcgcgg acggcttcct gtgttttcat tggtcgaagc
      121 gttctgcgcg tgcgcagaga ttccccaggg aaggcgggcc ctcttccgaa tgtccaatcc
      181 ggacattctt ccggttggac ctccgcggtc agcgctgtgc ccggcgagag atcacgtggg
      241 gcgcggaggc ggtgctgctg gggcacggcc gtccagcctc ggcggccata tttttgaggg
      301 gctgttcatc tcgttcacac gctctgtccg ccatgtttgt gagtggaagc gccattaccc
      361 cttcaagcga ctgaaggctg cagggcctct ggtggcccgc atggggagac cagacccgcc
      421 aggcccgcct ttccgcactc agtccgggct tactttattt tgtgagacag ggtctcgcct
      481 agaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      541 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      601 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      661 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      721 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      781 attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
      841 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
      901 ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
      961 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     1021 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     1081 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     1141 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     1201 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     1261 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     1321 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     1381 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     1441 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     1501 tgcgatggag tttccccata ctgagtgggt ggagactgaa gttaggccag cttggcactt
     1561 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     1621 tcagacagtg gttcaaagtt tttttcttcc atttaaggtg tcgtgaaaac taccccaagc
     1681 tggcctctgc ggccgccacc atggtaaaca gctacaatag actggaggcg atatcaactc
     1741 ttgttatact actctgggta actctggtcc agggttcctg tggcagctgt ccagctggta
     1801 ctttctggaa tggtacagat tgtggaaaaa acaaaaatca agcttgcatc ccatgtcccc
     1861 caaatagttt ctctagtaca gctggcgaac agaggacatg taacatatgc aggagatgtg
     1921 aaggtatttt tagaacaaag agagaatgta ctccaacaag taatacagag tgtgattgta
     1981 tgtctgggta tcattgttcc ggagtaggat gtaataggtg tcaaccagat tgcaagcaag
     2041 gtcaagaact tctaggaaaa ggttgtagag actgtagctt tggtaccttt aatgatcaga
     2101 cacgggggaa ctgcaaacgc tggacaaact gctctttgaa tggactagtt gtgcttgtaa
     2161 atggaaccaa taaaagagat gtgatatgtg gatccaccca aacagttgcc actctggaca
     2221 caatctccat gacaattcca gcttctctcg tgcacattga taagaaccat cagcccgggg
     2281 agaaccttta cttccaggga tccgctggct ccgctgctgg ttctggcgaa ttccatcatc
     2341 accatcacca tgtcgacgtg agcaagggcg aggagaataa catggccatc atcaaggagt
     2401 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     2461 gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag gtgaccaagg
     2521 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcacctac ggctccaagg
     2581 cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc cccgagggct
     2641 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2701 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2761 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     2821 accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg aaggacggcg
     2881 gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2941 gcgcctacat cgtcggcatc aagttggaca tcacctccca caacgaggac tacaccatcg
     3001 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     3061 agtaggctag cgtcaggcca agcttgggga tcaattctct agagctcgct gatcagcctc
     3121 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3181 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3241 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3301 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     3361 aagaaccagc tgggcctact agatagcgga ccccttaccg aaacatcgcc gcattctgca
     3421 gaggagtcga acttagtgta tgtaaacttc tgacccactg gaattgtgat acagtgaatt
     3481 ataagtgaaa taatctgtct gtaaacaatt gttggaaaaa tgacttgtgt catgcacaaa
     3541 gtagatgtcc taactgactt gccaaaacta ttgtttgtta acaagaaatt tgtggagtag
     3601 ttgaaaaacg agttttaatg actccaactt aagtgtatgt aaacttccga cttcaactgt
     3661 atagggatcc aattttaaca aaatattcga gcttgcatgc ctgcaggtcg ttacataact
     3721 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3781 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3841 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3901 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3961 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     4021 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     4081 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     4141 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     4201 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     4261 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     4321 actcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     4381 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     4441 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     4501 cggaattaat acgactcact atagggacta gtaccatggg aaaatcaaaa gaaatcagcc
     4561 aagacctcag aaaaagaatt gtagacctcc acaagtctgg ttcatccttg ggagcaattt
     4621 ccaaacgcct ggcggtacca cgttcatctg tacaaacaat agtacgcaag tataaacacc
     4681 atgggaccac gcagccgtca taccgctcag gaaggagacg cgttctgtct cctagagatg
     4741 aacgtacttt ggtgcgaaaa gtgcaaatca atcccagaac aacagcaaag gaccttgtga
     4801 agatgctgga ggaaacaggt acaaaagtat ctatatccac agtaaaacga gtcctatatc
     4861 gacataacct gaaaggccac tcagcaagga agaagccact gctccaaaac cgacataaga
     4921 aagccagact acggtttgca actgcacatg gggacaaaga tcgtactttt tggagaaatg
     4981 tcctctggtc tgatgaaaca aaaatagaac tgtttggcca taatgaccat cgttatgttt
     5041 ggaggaagaa gggggaggct tgcaagccga agaacaccat cccaaccgtg aagcacgggg
     5101 gtggcagcat catgttgtgg gggtgctttg ctgcaggagg gactggtgca cttcacaaaa
     5161 tagatggcat catggacgcg gtgcagtatg tggatatatt gaagcaacat ctcaagacat
     5221 cagtcaggaa gttaaagctt ggtcgcaaat gggtcttcca acacgacaat gaccccaagc
     5281 atacttccaa agttgtggca aaatggctta aggacaacaa agtcaaggta ttggagtggc
     5341 catcacaaag ccctgacctc aatcctatag aaaatttgtg ggcagaactg aaaaagcgtg
     5401 tgcgagcaag gaggcctaca aacctgactc agttacacca gctctgtcag gaggaatggg
     5461 ccaaaattca cccaaattat tgtgggaagc ttgtggaagg ctacccgaaa cgtttgaccc
     5521 aagttaaaca atttaaaggc aatgctacca aatactaggg gccctaaccg cggggatcca
     5581 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     5641 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     5701 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     5761 gaggtttttt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     5821 tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5881 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5941 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6001 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6061 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6121 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6181 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6241 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6301 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6361 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6421 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6481 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6541 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6601 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6661 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6721 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6781 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6841 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7681 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7741 gcgagtcagt gagcgaggaa gcggatccct atacagttga agtcggaagt ttacatacac
     7801 cttagccaaa tacatttaaa ctcacttttt cacaattcct gacatttaat cctagtaaaa
     7861 attccctgtc ttaggtcagt taggatcacc actttatttt aagaatgtga aatatcagaa
     7921 taatagtaga gagaatgatt catttcagct tttatttctt tcatcacatt cccagtgggt
     7981 cagaagttta catacactcg actcctctgc agaatgcggc gatgtttcgg taaggggtcc
     8041 cgagtagcta gttcatggca gccagcatat ggcatatgtt gccaaactct aaaccaaata
     8101 ctcattctga tgttttaaat gatttgccct cccatatgtc cttccgagtg agagacacaa
     8161 aaaattccaa cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg
     8221 ctcaaggggc ttcatgatgt ccccataatt tttggcagag ggaaaaagat ctcagtggta
     8281 tttgtgagcc agggcattgg ccacaccagc caccaccttc tgataggcag cctgcacctg
     8341 aggagtgaat tcacgacagg ccttcgaact attcctttgc cctcggacga gtgctggggc
     8401 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     8461 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     8521 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     8581 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     8641 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     8701 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     8761 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     8821 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     8881 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     8941 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     9001 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     9061 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     9121 agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga
     9181 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     9241 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     9301 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     9361 cgacagacgt cgcggtgagt tcaggctttt tcatggtggc ctcaggtgca gaggtttcta
     9421 cagggaaaga gagaaaggct acagttagtt tgctgtgatg atgctaagtg ccgaggtcag
     9481 atgtctatgg attgtacacg gaatcccaac gcaggaacca aaaaacactc acccaaaaaa
     9541 at
//
