LOCUS       sequence-270167-        9600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple 
            cloning sites to swap genes-of-interest (i.e. TNFSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF92
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1289..1993
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1431..1449)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1699..1718
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2000..2017
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2051..2071
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    2697..2921
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3283..3586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3587..3790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3741..3761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3787..3811
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3928..4024
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     4081..4100
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4081..4099
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4110..5132
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    5149..5283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5173..5192)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5227..5246
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5436..5540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5541..5609,5610..6401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5759..5778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6572..7160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7061..7080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7314..7331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7705..7724
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7723..7778
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7824..7843
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7943..8968)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7943..8968)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        join(9600,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccatactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt aaggtgtcgt gaaaactacc ccaagctggc
     1201 ctctgcggcc gccaccatgt acagcatgca gctcgcatcc tgtcttgcac tgacgctcgc
     1261 actccttgtc agcagtgcac ccgtcgacgt gagcaagggc gaggaggata acatggccat
     1321 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     1381 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     1441 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     1501 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     1561 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     1621 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     1681 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     1741 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     1801 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     1861 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     1921 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     1981 cgagctgtac aaggctagcc atcatcacca tcaccattcc gctggctccg ctgctggttc
     2041 tggcgaattc gagaaccttt acttccaggg acccgggagg gcaaatcttg cccaccctca
     2101 cgccgccgaa gggctctctg ccccccagtt tcctgctcag agcccctatg cccagctggt
     2161 gatgaaagat gccttggtgc agaaccagac actgagctgg cacaatgatc ctcatctttc
     2221 tcgtgtgttc ctggacccac gaatgaatta cgatgctgat aaaggagagc tggaggtcaa
     2281 agtcgccggg ctttacttcg tctacactca cctgaagctg cagcaggttg tggttcctgc
     2341 atacagtcaa ggtacagtcg aggtctttgt gactgtggtg caggactttg gacagcgagc
     2401 tgtcttgaat ctctccctgg atgtgggacc ttccagcact tccaccatca ccacctcgga
     2461 gtcctccctc atcttcctgc atcctgggga gcggctcagg ctcagcatga gcgccacagc
     2521 aggggacttc ctggactggc agctggtgtc aggggccaat acctttggcc ttttctgggt
     2581 ggccggggga cagcccttaa ccgattctgg gacattaact gtgatcaatt aggtttaaac
     2641 ggcctgtcag gccaagcttg gggatcaatt ctctagagct cgctgatcag cctcgactgt
     2701 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2761 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2821 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2881 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     2941 cagctgggcc tactagatag cggacccctt accgaaacat cgccgcattc tgcagaggag
     3001 tcgaacttag tgtatgtaaa cttctgaccc actggaattg tgatacagtg aattataagt
     3061 gaaataatct gtctgtaaac aattgttgga aaaatgactt gtgtcatgca caaagtagat
     3121 gtcctaactg acttgccaaa actattgttt gttaacaaga aatttgtgga gtagttgaaa
     3181 aacgagtttt aatgactcca acttaagtgt atgtaaactt ccgacttcaa ctgtataggg
     3241 atccaatttt aacaaaatat tcgagcttgc atgcctgcag gtcgttacat aacttacggt
     3301 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3361 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3421 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3481 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3541 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3601 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3661 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3721 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3781 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3841 cctccataga agacaccggg accgatccag cctccggact ctagaggatc cggtactcga
     3901 ggaactgaaa aaccagaaag ttaactggta agtttagtct ttttgtcttt tatttcaggt
     3961 cccggatccg gtggtggtgc aaatcaaaga actgctcctc agtggatgtt gcctttactt
     4021 ctaggcctgt acggaagtgt tacttctgct ctaaaagctg cggaattgta cccgcggaat
     4081 taatacgact cactataggg actagtacca tgggaaaatc aaaagaaatc agccaagacc
     4141 tcagaaaaag aattgtagac ctccacaagt ctggttcatc cttgggagca atttccaaac
     4201 gcctggcggt accacgttca tctgtacaaa caatagtacg caagtataaa caccatggga
     4261 ccacgcagcc gtcataccgc tcaggaagga gacgcgttct gtctcctaga gatgaacgta
     4321 ctttggtgcg aaaagtgcaa atcaatccca gaacaacagc aaaggacctt gtgaagatgc
     4381 tggaggaaac aggtacaaaa gtatctatat ccacagtaaa acgagtccta tatcgacata
     4441 acctgaaagg ccactcagca aggaagaagc cactgctcca aaaccgacat aagaaagcca
     4501 gactacggtt tgcaactgca catggggaca aagatcgtac tttttggaga aatgtcctct
     4561 ggtctgatga aacaaaaata gaactgtttg gccataatga ccatcgttat gtttggagga
     4621 agaaggggga ggcttgcaag ccgaagaaca ccatcccaac cgtgaagcac gggggtggca
     4681 gcatcatgtt gtgggggtgc tttgctgcag gagggactgg tgcacttcac aaaatagatg
     4741 gcatcatgga cgcggtgcag tatgtggata tattgaagca acatctcaag acatcagtca
     4801 ggaagttaaa gcttggtcgc aaatgggtct tccaacacga caatgacccc aagcatactt
     4861 ccaaagttgt ggcaaaatgg cttaaggaca acaaagtcaa ggtattggag tggccatcac
     4921 aaagccctga cctcaatcct atagaaaatt tgtgggcaga actgaaaaag cgtgtgcgag
     4981 caaggaggcc tacaaacctg actcagttac accagctctg tcaggaggaa tgggccaaaa
     5041 ttcacccaaa ttattgtggg aagcttgtgg aaggctaccc gaaacgtttg acccaagtta
     5101 aacaatttaa aggcaatgct accaaatact aggggcccta accgcgggga tccagacatg
     5161 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5221 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5281 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5341 ttttaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5401 acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5521 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5581 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5641 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5701 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5761 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5821 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5881 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5941 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6001 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6061 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6121 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6181 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6241 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6301 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6361 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6421 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6481 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6541 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6601 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6661 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6721 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6781 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6841 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6901 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6961 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7021 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7081 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7141 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7201 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7261 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7321 cagtgagcga ggaagcggat ccctatacag ttgaagtcgg aagtttacat acaccttagc
     7381 caaatacatt taaactcact ttttcacaat tcctgacatt taatcctagt aaaaattccc
     7441 tgtcttaggt cagttaggat caccacttta ttttaagaat gtgaaatatc agaataatag
     7501 tagagagaat gattcatttc agcttttatt tctttcatca cattcccagt gggtcagaag
     7561 tttacataca ctcgactcct ctgcagaatg cggcgatgtt tcggtaaggg gtcccgagta
     7621 gctagttcat ggcagccagc atatggcata tgttgccaaa ctctaaacca aatactcatt
     7681 ctgatgtttt aaatgatttg ccctcccata tgtccttccg agtgagagac acaaaaaatt
     7741 ccaacacact attgcaatga aaataaattt cctttattag ccagaagtca gatgctcaag
     7801 gggcttcatg atgtccccat aatttttggc agagggaaaa agatctcagt ggtatttgtg
     7861 agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggagt
     7921 gaattcacga caggccttcg aactattcct ttgccctcgg acgagtgctg gggcgtcggt
     7981 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     8041 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     8101 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     8161 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     8221 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     8281 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     8341 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     8401 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     8461 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     8521 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     8581 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     8641 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     8701 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     8761 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     8821 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     8881 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     8941 acgtcgcggt gagttcaggc tttttcatgg tggcctcagg tgcagaggtt tctacaggga
     9001 aagagagaaa ggctacagtt agtttgctgt gatgatgcta agtgccgagg tcagatgtct
     9061 atggattgta cacggaatcc caacgcagga accaaaaaac actcacccaa aaaaatggcg
     9121 ctaaggccga gagaaaggcg aagttccgtc tacggctata taccttgaaa caggggcggc
     9181 agaagtgacg caacgcggtt gcgcggacgg cttcctgtgt tttcattggt cgaagcgttc
     9241 tgcgcgtgcg cagagattcc ccagggaagg cgggccctct tccgaatgtc caatccggac
     9301 attcttccgg ttggacctcc gcggtcagcg ctgtgcccgg cgagagatca cgtggggcgc
     9361 ggaggcggtg ctgctggggc acggccgtcc agcctcggcg gccatatttt tgaggggctg
     9421 ttcatctcgt tcacacgctc tgtccgccat gtttgtgagt ggaagcgcca ttaccccttc
     9481 aagcgactga aggctgcagg gcctctggtg gcccgcatgg ggagaccaga cccgccaggc
     9541 ccgcctttcc gcactcagtc cgggcttact ttattttgtg agacagggtc tcgcctagag
//
