LOCUS       sequence-27018-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot 
            JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
            Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen 
            HS, Mercola M
  TITLE     Lentiviral vectors and protocols for creation of stable hESC lines 
            for fluorescent tracking and drug resistance selection of 
            cardiomyocytes.
  JOURNAL   PLoS ONE. 2009  . 4(4):e5046.
  PUBMED    19352491
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2009  . 4(4):e5046."
COMMENT     Sequence Label: PGK-H2BeGFP
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(34..411)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     primer_bind     complement(483..501)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tggtggcgac cggtttagcg ctggtgtact tggtgacggc
       61 cttagtaccc tcggacacgg cgtgcttggc caactcccca ggcagcagca ggcgcacggc
      121 cgtctggatc tccctggagg tgatggtcga gcgcttgttg taatgcgcca ggcgggaagc
      181 ctcacctgcg atgcgctcga aaatgtcgtt cacaaacgaa ttcatgatgc ccatggcctt
      241 ggacgaaatg ccggtgtcag ggtggacctg cttcagaacc ttgtacacat agatggaata
      301 gctctccttg cggctgcgct tgcgcttctt gccgtctttc ttctgcgcct tagtcaccgc
      361 cttcttggag ccctttttcg gggcgggagc agacttcgct ggctctggca tggatccccc
      421 tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga
      481 ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac
      541 cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg
      601 ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct
      661 gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc
      721 aaggaacctt cccgacttag gggcgga
//
