LOCUS       sequence-270189-        3722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PDZ mCherry DrTrkA expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pPDZ-mCherry-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..3722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             313..1020
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(458..476)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     726..745
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggcaaaac aagagattcg agtgagggtt gaaaaggacc cagaacttgg atttagcata
       61 tcaggtggtg tcgggggtag aggaaaccca ttcagacctg atgatgatgg tatatttgta
      121 acaagggtac aacctgaagg accagcatca aaattactgc agccaggtga taaaattatt
      181 caggctaatg gctacagttt tataaatatt gaacatggac aagcagtgtc cttgctaaaa
      241 actttccaga atacagttga actcatcatt gtacgagaag tttctagact cgagagcgcg
      301 ggcagcgcgg gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      361 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      481 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      541 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      601 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      661 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
      781 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
      841 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      901 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      961 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1021 gaattcagtg ctggtagtgc tggtagtgct ggcaccggta tgagccggga cccgttgccc
     1081 ttttttccac cgctttacct tggtggcccg gaaattacca ccgagaactg cgagcgcgag
     1141 ccgattcata ttcccggcag catccagccg cacggcgccc tgctcactgc cgacgggcac
     1201 agcggcgagg tgctccagat gagcctcaac gcggccactt ttctgggaca ggaacccaca
     1261 gtgctgcgcg gacagaccct cgccgcactg ctgcccgagc agtggcccgc gctgcaagcg
     1321 gccctgcccc ccggctgccc cgacgccctg caataccgcg caacgctgga ctggcctgcc
     1381 gccgggcacc tttcgctgac ggtgcaccgg gtcggcgagt tgctgattct ggagttcgag
     1441 ccgacggagg cctgggacag caccgggccg cacgcgctgc gcaacgcgat gttcgcgctc
     1501 gaaagtgccc ccaacctgcg ggcgctggcc gaggtggcga cccagacggt ccgcgagctg
     1561 acgggctttg accgggtgat gctctacaaa tttgcccccg acgccaccgg cgaagtgatt
     1621 gccgaggccc gccgtgaggg gctgcacgcc tttctgggcc accgttttcc cgcgtcggac
     1681 attccggcgc aggcccgcgc gctctacacc cggcacctgc tgcgcctgac cgccgacacc
     1741 cgcgccgccg ccgtgccgct cgatcccgtc ctcaacccgc agacgaatgc gcccaccccg
     1801 ctgggcggcg ccgtgctgcg cgccacctcg cccatgcaca tgcagtacct gcggaacatg
     1861 ggcgtcgggt cgagcctgtc ggtgtcggtg gtggtcggcg gccagctctg gggcctgatc
     1921 gcctgccacc accagacgcc ctacgtgttg ccgcccgacc tgcgaaccac gctcgaatac
     1981 ctgggccgct tgctgagcct gcaagttcag gtcaaggaag cggcggacgt ggcggccttt
     2041 cgccagagcc tgcgggagca ccacgcgcgg gtggccctcg cggcggcgca ctcgctctcg
     2101 ccgcacgaca ccctcagtga cccggcgctt gacctgctgg gcctgatgcg ggccgggggc
     2161 ctgattctgc gtttcgaggg ccgctggcag acgttgggtg aagtgccgcc tgccccggcg
     2221 gtggacgcgc tgctggcgtg gctcgaaacc cagccgggcg ccctggtcca gaccgacgcg
     2281 ctaggccaac tgtggcccgc cggcgccgat ctcgccccca gcgcagcggg cctgctcgcc
     2341 atcagcgtgg gcgagggctg gtcggagtgc ctcgtctggc tgcggcccga actgcggctg
     2401 gaggtcgcct ggggcggggc cactcctgac caggcgaaag acgacctcgg gccgcgccac
     2461 tcattcgaca cctacctcga agaaaaacgc ggctacgccg agccctggca tcccggcgaa
     2521 atcgaggagg cgcaggatct acgtgacaca ttgaccgggg cgctgggcga ggcagaggca
     2581 gcagcaaagg aggcagcagc aaaggaagcg gcggcgaaag aagcggcggc gaaagcgaac
     2641 aaatgtggac agaggagcaa atttgggatc aaccgccctg ctgtgctggc gccagaggat
     2701 gggctggcca tgtccctaca cttcatgaca ctgggtggca gttctctttc ccctactgag
     2761 ggcaaaggct ccggactcca gggccacatc atggagaacc cacagtactt cagtgatacc
     2821 tgtgtccacc atatcaagcg ccaggacatc attctcaagt gggagctagg ggagggagcc
     2881 tttggaaagg tctttcttgc tgagtgctac aaccttctga atgatcagga caagatgctg
     2941 gtggctgtca aggcactgaa ggagacatct gagaatgctc gtcaggactt ccatcgtgag
     3001 gcagagctgc tcaccatgct acagcaccaa cacatcgtac gcttctttgg agtctgcacg
     3061 gagggtggcc cattgctcat ggtcttcgag tacatgcgcc atggggacct caaccgtttc
     3121 ctccggtccc acggacctga tgcaaaactg ctggctggcg gcgaggatgt ggctcctggt
     3181 cctttgggcc ttgggcagct tctggctgtg gctagccagg tggctgctgg tatggtgtac
     3241 ctagccagcc tgcactttgt gcaccgggac ctggccacac gcaactgtct ggtgggtcag
     3301 ggactagtgg tcaagattgg agacttcggc atgagcaggg acatctacag cacagactac
     3361 taccgtgtgg gaggtcggac catgctgccc atccgctgga tgcctccaga gagcatcctc
     3421 taccgcaagt tcagcaccga gagtgatgtg tggagcttcg gggtggtgct ctgggagatc
     3481 ttcacctatg gaaagcaacc ctggtaccag ctctccaaca ctgaggcgat cgagtgcatc
     3541 acgcagggcc gggagctgga gcggccgcgc gcctgccctc ctgatgtcta cgccatcatg
     3601 cgcggctgct ggcagcgtga gccgcaacag cgcctcagca tgaaggatgt gcacgcgcgg
     3661 ctgcaggcct tggcacaggc gccaccgagt tacctggacg ttctgggcgg atccactagt
     3721 aa
//
