LOCUS       sequence-270197-        9249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid can be used to express independently eGFP and TagRFP 
            from a bidirectional 5xUAS. Also CFP expression under crystallin 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Sanz-Morejon A, Garcia-Redondo AB, Reuter H, Marques IJ, Bates T, 
            Galardi-Castilla M, Grosse A, Manig S, Langa X, Ernst A, Piragyte I,
            Botos MA, Gonzalez-Rosa JM, Ruiz-Ortega M, Briones AM, Salaices M, 
            Englert C, Mercader N
  TITLE     Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage 
            Subtype and Is Required for Organ Regeneration in the Zebrafish.
  JOURNAL   Cell Rep. 2019 Jul 30;28(5):1296-1306.e6. doi: 
            10.1016/j.celrep.2019.06.091.
  PUBMED    31365871
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.06"; journalName: "Cell Rep"; date: 
            "2019-07-30- 30"; volume: "28"; issue: "5"; pages: "1296-1306.e6"
COMMENT     Sequence Label: Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol...
FEATURES             Location/Qualifiers
     source          1..9249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(574..1305)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    1462..1556
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      1632..1641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1638..1640,1641..1643,1644..2357)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1638..1640,1641..1643,1644..2354)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     intron          2420..2992
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    3065..3199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3333..3467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3573..3707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3843..4556,4557..4559,4560..4562))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(5193..5380,5381..5392)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5426..5442)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5426..5442)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5479..5499)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5479..5497)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5518..5534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5518..5534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5531..5553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5542..5558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5566..5572,5573..5590,5591..5596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5611..5632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5749..5766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5920..6508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6000..6019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6679..7470,7471..7539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7302..7321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7540..7644)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7807..7828)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8281..8292,8293..8455)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      8848..8857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8852..8861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8986..9120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          join(9193..9249,1..516)
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 agagtatttt atcctcatct caaacagtgt atatcattgt aaccataaag agaaaggcag
       61 gatgatgacc agggtgtagt tgtttctacc aataagaata tttccacgcc agccagaatt
      121 tatatgcaga aatattctac cttatcattt aattataaca attgttctct aaaactgtgc
      181 tgaagtacaa tataatatac cctgattgcc ttgaaaaaaa agtgattaga gaaagtactt
      241 acaatctgac aaataaacaa aagtgaattt aaaaattcgt tacaaatgca agctaaagtt
      301 taacgaaaaa gttacagaaa atgaaaagaa aataagagga gacaatggtt gtcaacagag
      361 tagaaagtga aagaaacaaa attatcatga gggtccatgg tgatacaagg gacatcttcc
      421 cattctaaac aacaccctga aaactttgcc ccctccatat aacatgaatt ttacaatagc
      481 gaaaaagaaa gaacaatcaa gggtccccaa actcaccctg aagttctcag gatcggtcga
      541 gtaatacgac tcactatagt tctagctaga ttacttgtac agctcgtcca tgccattaag
      601 tttgtgcccc agtttgctag ggaggtcgca gtatctggcc acagccacct cgtgctgctc
      661 gacgtaggtc tctttgtcgg cctccttgat tctttccagt ctgtggtcca catagtagac
      721 gccgggcatc ttgaggttct tagcgggttt cttggatctg tatgtggtct tgaagttgca
      781 gatcaggtgg cccccgccca cgagcttcag ggccatgtcg gttctgcctt ccaggccgcc
      841 gtcagcgggg tacagcatct cggtgttggc ctcccagccg agtgttttct tctgcatcac
      901 agggccgttg gatgggaagt tcacccctct gatcttgacg ttgtagatga ggcagccgtc
      961 ctggaggctg gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg tggtgactct
     1021 ctcccatgtg aagccctcag ggaaggactg cttaaagaag tcggggatgc cctgggtgtg
     1081 gttgatgaag gttctgctgc cgtacatgaa gctggtagcc aggatgtcga aggcgaaggg
     1141 gagagggccg ccctcgacca ccttgattct catggtctgg gtgccctcgt agggcttgcc
     1201 ttcgccctcg gatgtgcact tgaagtggtg gttgttcacg gtgccctcca tgtacagctt
     1261 catgtgcatg ttctccttaa tcagctcttc gcccttagac accatcctcg acgtcagttt
     1321 tgttgcttct ttatccttac attgcacaca gcccatggtg gaattgttaa ttaatttaaa
     1381 tcaattcgtg tggaggagct caaagtgagg ctgagacgcg atgggatcca ttatataccc
     1441 tctagatcca tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc
     1501 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     1561 gactctagag ggtatataat ggatcccatc gcgtctcagc ctcactttga gctcctccac
     1621 acgaattcgc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1681 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1741 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1801 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1861 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1921 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1981 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2041 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2101 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2161 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2221 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2281 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2341 acgagctgta caagtaactc gagcctctag ctagaactat agtgagtcgt attactcgac
     2401 cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     2461 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     2521 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     2581 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     2641 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     2701 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     2761 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     2821 tggctggcgt ggaaatattc ttattggtag aaacaactac accctggtca tcatcctgcc
     2881 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     2941 caaaccgggc ccctctgcta accatgttca tgccttcttc tctttcctac agctcctggg
     3001 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattaattc ctcgacggat
     3061 cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3121 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3181 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3241 ggaggtgtgg gaggtttttt aattcgcggc cggccgcggc gccaatgcat tgggcccggt
     3301 acctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     3361 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3421 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaattgca
     3481 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg
     3541 cgacctgcag cctgttgaca attttcgatg atgatccaga catgataaga tacattgatg
     3601 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3661 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3721 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3781 acctctacaa atgtggtatg gctgattatg atcctctaga tcagatccag agtcgcggcc
     3841 gcttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     3901 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gcttggactg ggtgctcagg
     3961 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     4021 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg
     4081 ccgttcttct gcttgtcggc ggtgatatag acgttgtcgc tgatggcgtt gtactccagc
     4141 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     4201 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     4261 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     4321 tgcttcatgt ggtcggggta gcgggcgaag cactgcacgc cccaggtcag ggtggtcacg
     4381 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     4441 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     4501 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
     4561 atggtacccc cgcaagtctg actgagtgga agtttcagtt caatggaagg gagaaaagag
     4621 ccagccttta tatatgtcat gtattgctgc tgtctgcatg cgatgaacct agagcttact
     4681 ttttctgtca agtaaggcaa aagcagagaa cctttgtctt gcattgtgtt tgatgttgtg
     4741 ctgtgacagt catttcttag ttcatgctgg tacttttgtt ccatagcttt acccaaagag
     4801 ttatccagca ttcccagtta ttccaaaaac tcaatgtcac aaattttcca gtccattgct
     4861 tagcagccga gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg
     4921 gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt
     4981 gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg
     5041 acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa
     5101 acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa
     5161 ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt
     5221 tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta
     5281 agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat
     5341 aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga
     5401 ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg
     5461 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     5521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6541 cgctcagtgg aacgaaaact cacgttaagg cattttggtc atgagattat caaaaaggat
     6601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     7681 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     7741 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     7801 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     7861 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     7921 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     7981 gcttgacggg gaaaggctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta
     8041 ccaatgcacc aattggccac aatgacggct actacatggt gccattcctt cctctttata
     8101 ggaatggaga ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg
     8161 acccaggtca ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg
     8221 ttcagacacc catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga
     8281 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
     8341 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
     8401 agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa
     8461 actagagatt cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg
     8521 ttttatcctg ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa
     8581 tgccagtaca cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt
     8641 ctcaaaattg ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct
     8701 gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct
     8761 gcaaatagca ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc
     8821 cctcgagccc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgattcaca
     8881 cacgccgtac cgggcccaat gcattggcgc cgcggccgcg aattaaaaaa cctcccacac
     8941 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9121 tacgatccgt cgaggaatta attctttgcc aaaatgatga gacagcacaa caaccagcac
     9181 gttgcccagg agctgtagga aagagaagaa ggcatgaaca tggttagcag aggggcccgg
     9241 tttggactc
//
